Novel VEGF-like factor

Abstract

A novel human gene having a significant homology with a VEGF-C gene, a member of the VEGF family, has been isolated by the PCR method using primers designed based on the sequence of EST that is assumed to be homologous with the C-terminal region of the VEGF-C gene. Mouse and rat genes have been isolated based on the human gene isolated as above. A protein encoded by the above human gene has been isolated by introducing the gene into Escherichia coli and expressing it. The isolated protein and genes can be applied to, for example, gene therapy for the VEGF-D deficiency, wound healing, and promotion of collateral vessel formation. Furthermore, VEGF-D protein inhibitors can be used as a novel anticancer drug, etc.

1 . canceled.  
     
     
         2 . canceled.  
     
     
         3 . A DNA encoding a protein shown by SEQ ID NO: 1 or having the amino acid sequence derived therefrom in which one or more amino acids are substituted, deleted, or added.  
     
     
         4 . A DNA hybridizing with the DNA shown by SEQ ID NO: 2.  
     
     
         5 . A vector containing the DNA of  claim 3  or 4.  
     
     
         6 . A transformant carrying the vector of  claim 5 .  
     
     
         7 . A method of producing a protein shown by SEQ ID NO: 1 or having the amino acid sequence derived therefrom in which one or more amino acids are substituted, deleted, or added or a protein encoded by a DNA hybridizing with the DNA shown by SEQ ID NO:2, wherein said method comprises culturing the transformant of  claim 6 .  
     
     
         8 . canceled.  
     
     
         9 . canceled.  
     
     
         10 . canceled.