Complementary DNAs

Abstract

The sequences of extended cDNAs encoding secreted proteins are disclosed. The extended cDNAs can be used to express secreted proteins or portions thereof or to obtain antibodies capable of specifically binding to the secreted proteins. The extended cDNAs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. The extended cDNAs may also be used to design expression vectors and secretion vectors.

1 . A purified or isolated nucleic acid comprising: 
 a) at least 10 consecutive bases of one of SEQ ID NOs: 40-84, 130-154, or a sequence complementary thereto;    b) the sequence of one of SEQ ID NOs: 40-84, 130-154, or a sequence complementary thereto;    c) the full coding sequence of one of SEQ ID NOs: 40-59, 61-73, 75, 77-82 or 130-154 wherein the full coding sequence comprises the sequence encoding signal peptide and the sequence encoding mature protein;    d) the nucleotides of one of SEQ ID NOs: 40-59, 61-73, 75-82, 84 or 130-154 which encode a signal peptide;    e) the nucleotides of one of SEQ ID NOs: 40-59, 61-73, 75-82, 84 or 130-154 which encode a mature peptide;    f) a polynucleotide sequence encoding at least 10 amino acids of a polypeptide having the sequence of one of the sequences of SEQ ID NOs: 85-129 or 155-179;    g) a polynucleotide sequence encoding a polypeptide having the sequence of one of the sequences of SEQ ID NOs: 85-104, 106-120, 122-127 or 155-179;    h) a polynucleotide sequence encoding a signal peptide included in one of the sequences of SEQ ID NOs: 85-104, 106-118, 120-127, 129 or 155-179; or    i) a vector comprising a polynucleotide as set forth in 1a), 1b), 1c), 1d), 1e), 1f) 1g) or 1h).    
     
     
         2 . A purified or isolated polypeptide comprising: 
 a) at least 10 consecutive amino acids of one of the sequences of SEQ ID NOs: 85-129 or 155-179;    b) the full length sequence of one of SEQ ID NOs: 85-129 or 155-179; or    c) a signal peptide of one of the polypeptides of SEQ ID NOs: 85-104, 106-118, 120-127, 129 or 155-179;    
     
     
         3 . A host cell comprising a polynucleotide or vector according to  claim 1 .  
     
     
         4 . A method of making a protein comprising one of the sequences of SEQ ID NO: 85-129 and 155-179, comprising the steps of: a) growing a host cell according to  claim 3  under conditions whereby said protein is expressed, and b) isolating said protein.  
     
     
         5 . In an array of polynucleotides of at least 15 nucleotides in length, the improvement comprising inclusion in said array of at least one of the sequences of SEQ ID NOs: 40-84 and 130-154, or one of the sequences complementary to the sequences of SEQ ID NOs: 40-84 and 130-154, or a fragment thereof of at least 15 consecutive nucleotides.  
     
     
         6 . A purified or isolated antibody capable of binding to a polypeptide comprising at least 10 consecutive amino acids of the sequence of one of SEQ ID NOs: 85-129 or 155-179.  
     
     
         7 . A method of binding an antibody to an antigen comprising contacting an antibody capable of binding to a polypeptide comprising at least 10 consecutive amino acids of the sequence of one of SEQ ID NOs: 85-129 or 155-179 with a polypeptide comprising at least 10 consecutive amino acids of the sequence of one of SEQ ID NOs: 85-129 or 155-179.