Novel kinases and uses thereof

Abstract

Novel kinase polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length kinase proteins, the invention further provides isolated kinase fusion proteins, antigenic peptides, and anti-kinase antibodies. The invention also provides kinase nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a kinase gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

1 . An isolated polypeptide selected from the group consisting of: 
 a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2;    b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, wherein the fragment has a kinase protein activity and comprises at least 75 contiguous amino acids of SEQ ID NO:2;    c) a polypeptide having a kinase protein activity, wherein said polypeptide is encoded by a nucleic acid molecule comprising a nucleotide sequence that is at least 85% identical to SEQ ID NO:1, nucleotides 191-1156 of SEQ ID NO:1, or a complement thereof; and    d) a polypeptide having a kinase protein activity, wherein the polypeptide is encoded by a nucleic acid molecule that hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, nucleotides 191-1156 of SEQ ID NO:1, or a complement thereof under stringent conditions, said stringent conditions comprising hybridization in 6×SSC at 42° C., followed by washing with 1×SSC at 55° C.    
     
     
         2 . The isolated polypeptide of  claim 1  comprising the amino acid sequence of SEQ ID NO:2.  
     
     
         3 . The polypeptide of  claim 1  further comprising heterologous amino acid sequences.  
     
     
         4 . An antibody that selectively binds to a polypeptide of  claim 1 .  
     
     
         5 . An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.  
     
     
         6 . The polypeptide of  claim 5  further comprising heterologous amino acid sequences.  
     
     
         7 . An antibody that selectively binds to the polypeptide of  claim 5 .  
     
     
         8 . A polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, wherein the fragment has a kinase protein activity and comprises at least 75 contiguous amino acids of SEQ ID NO:2.  
     
     
         9 . The polypeptide of  claim 8  further comprising heterologous amino acid sequences.  
     
     
         10 . An antibody that selectively binds to the polypeptide of  claim 8 .  
     
     
         11 . A polypeptide having a kinase protein activity, wherein said polypeptide is encoded by a nucleic acid molecule comprising a nucleotide sequence that is at least 85% identical to SEQ ID NO:1, nucleotides 191-1156 of SEQ ID NO:1, or a complement thereof.  
     
     
         12 . The polypeptide of  claim 11  further comprising heterologous amino acid sequences.  
     
     
         13 . An antibody that selectively binds to the polypeptide of  claim 11 .  
     
     
         14 . A polypeptide having a kinase protein activity, wherein the polypeptide is encoded by a nucleic acid molecule that hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, nucleotides 191-1156 of SEQ ID NO:1, or a complement thereof under stringent conditions, said stringent conditions comprising hybridization in 6×SSC at 42° C., followed by washing with 1×SSC at 55° C.  
     
     
         15 . The polypeptide of  claim 14  further comprising heterologous amino acid sequences.  
     
     
         16 . An antibody that selectively binds to the polypeptide of  claim 14.