Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof

Abstract

The present invention absorbs and captures nucleic components from various specimens by a single stationary phase and finally recovers the nucleic components by eluting the captured nucleic components from the stationary phase. This invention enables recovery of nucleic components without reducing the concentrations of nucleic acids and detection of specific nucleic components such as specific viruses.

1 - 11 . (canceled)  
     
     
         12 . A nucleic acid recovering apparatus which applies specimens containing nucleic components in an area to a stationary phase capable of binding with said nucleic components to make said nucleic components be absorbed and bound by said stationary phase and elutes said nucleic components from said stationary phase for recovery, comprising means to scan said area in a predetermined sequence, 
 first means to supply said specimen to said area, second means to remove components which are left unabsorbed from said stationary phase,    third means to supply a washing liquid to said area, and    means to apply an eluting solution to said stationary phase; wherein    the apparatus controls to perform said first, second, and third means in sequence, to cause an identical stationary phase to absorb and bind with target nucleic components, and to apply an eluting solution to said stationary phase to elute nucleic components from said stationary phase.    
     
     
         13 . A nucleic acid recovering apparatus in accordance with  claim 12 , further comprising means to add a substance which accelerates nucleic components having a specific structure to be bound with the stationary phase before applying said specimen to said stationary phase.  
     
     
         14 . A nucleic acid recovering apparatus in accordance with  claim 12 , wherein said area is a capillary having a capillary tip at the lower end and the capillary tip contains said stationary phase in it.  
     
     
         15 . A nucleic acid recovering apparatus in accordance with  claim 14 , wherein said tip can be mounted on and demounted from said capillary.