Organic anion transporter genes and proteins

Abstract

This invention relates to novel human organic anion transporter polypeptides and genes encoding such polypeptides. The invention is directed towards the isolation, characterization and use of human organic anion transporter polypeptides for pharmacological screening of substrates, analogues of substrates, and modulators of human organic anion proteins. This invention also provides cell cultures, which stably contain nucleic acid sequences encoding human organic anion transport polypeptides. Such cell lines are useful for screening compounds in vitro to identify and characterize modulators, inhibitors and substrates of human organic anion transporter polypeptides.

1 - 33 . (canceled)  
     
     
         34 . An isolated, enriched, or purified human OAT polypeptide encoded by the nucleic acid sequence set forth in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, or SEQ ID NO:6, or the amino acid sequence set forth in SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, or SEQ ID NO:12.  
     
     
         35 . The polypeptide of  claim 34 , wherein said polypeptide is a fragment of the polypeptide encoded by the nucleic acid sequence set forth in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, or SEQ ID NO:6, or the amino acid sequence set forth in SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, or SEQ ID NO:12.  
     
     
         36 . The polypeptide of  claim 34 , wherein said polypeptide is isolated, purified, or enriched from a cell that comprises an endogenous nucleic acid molecule that encodes said polypeptide.  
     
     
         37 . The polypeptide of  claim 34 , wherein said polypeptide is isolated, purified, or enriched from a cell that is transformed with a nucleic acid molecule that encodes said polypeptide.  
     
     
         38 . The polypeptide of  claim 34 , wherein said polypeptide is chemically synthesized.  
     
     
         39 - 54 . (canceled)