Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety

Abstract

Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

1 . A method of manufacturing a microfluidic device, the method comprising: 
 (a) providing a first substrate comprising a surface with microscale grooves fabricated into the surface;    (b) adding a first coating subunit to the surface; and    (c) adding a second coating subunit that binds to the first coating subunit.    
     
     
         2 . The method of  claim 1 , wherein the first coating subunit is added to a first region of the surface but not to a second region of the surface, and wherein the second coating subunit is added to both the first and second regions of the surface.  
     
     
         3 . The method of  claim 1 , wherein the first substrate comprises glass.  
     
     
         4 . The method of  claim 1 , wherein the first coating subunit comprises a silicon-based linker arm.  
     
     
         5 . The method of  claim 4 , wherein the silicon-based linker arm terminates in an amine moiety.  
     
     
         6 . The method of  claim 1 , wherein the second coating subunit is a monocarboxy poly(ethyleneglycol).  
     
     
         7 . The method of  claim 1 , wherein the first substrate comprises a polymeric material.  
     
     
         8 . The method of  claim 7 , wherein the polymeric material is polymethylmethacrylate (PMMA).  
     
     
         9 . The method of  claim 7 , wherein the polymeric material is polydimethlysiloxane (PDMS).  
     
     
         10 . The method of  claim 1 , further comprising the step of binding a second substrate to the first substrate.