Chemically modified biological molecules and methods for coupling biological molecules to solid support

Abstract

The invention relates to novel chemically modified biological molecules with enhanced lability towards solid supports, such as glass. These modified molecules can be readily affixed to solid supports, for instance, a glass surface, without first derivatizing the glass surface. High-density microarrays based on these modified molecules as well as methods for preparing these microarrays are also useful.

1 . A high-density microarray comprising: a solid support and a modified biological molecule wherein said molecule is immobilized about said solid support in orderly discrete spots.  
     
     
         2 . The high-density microarray of  claim 1  wherein said solid support is an inert material.  
     
     
         3 . The high-density microarray of  claim 2  wherein said solid support is selected from the group consisting of glass, metal, or plastic.  
     
     
         4 . The high-density microarray of  claim 1  wherein said molecule is selected from the group consisting of DNA, RNA, peptides, proteins, saccharides, polysaccarides, and lipids.  
     
     
         5 . The high-density microarray of  claim 1  wherein said discrete spots are about 50 microns in diameter.  
     
     
         6 . A high-density microarray comprising: the modified biological molecule of  claim 1;  and a solid support upon which said plurality of closely spaced samples of said biological samples are placed.  
     
     
         7 . A method for immobilizing a biological molecule to a solid support comprising: reacting a biological molecule to form a derivatized biological molecule, and reacting said derivatized molecule with said solid support.