US2005059131A1PendingUtilityA1

Detergent compositions containing amylase variants

54
Assignee: NOVOZYMES ASPriority: Feb 3, 1995Filed: Feb 12, 2004Published: Mar 17, 2005
Est. expiryFeb 3, 2015(expired)· nominal 20-yr term from priority
Inventors:Henrik Bisgard-FrantzenAllan SvendsenTorben Vedel Borchert
C11D 3/38618D06L 1/14C12N 9/2417C11D 3/38609C12Y 302/01001C11D 3/38681
54
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Claims

Abstract

The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca 2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

Claims

exact text as granted — not AI-modified
1 - 76 . (Canceled.)  
     
     
         77 . A detergent comprising a surfactant and an alpha-amylase, wherein the alpha-amylase is selected from the group consisting of: 
 an alpha-amylase comprising an amino acid sequence having at least 80% homology to SEQ ID NO:1 and which alpha-amylase Is modified by having an amino acid deletion of two amino adds selected from the group of amino adds equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:1;    an alpha-amylase comprising an amino acid sequence having at least 80% homology to SEQ ID NO:2 and which alpha-amylase is modified by having an amino acid deletion of two amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:2;    an alpha-amylase comprising an amino acid sequence having at least 80% homology to SEQ ID NO:3 and which alpha-amylase is modified by having an amino acid deletion of two amino acids selected from the group of amino acids equivalent to positions 178, 179, 180, 181, 182 and 183 in SEQ ID NO:3; and    an alpha-amylase comprising an amino acid sequence having at least 80% homology to SEQ ID NO:7 and which alpha-amylase is modified by having an amino acid deletion of two amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:7.    
     
     
         78 . A detergent comprising a surfactant and an alpha-amylase, wherein the alpha-amylase has an amino add sequence having at least 80% homology to SEQ ID NO:1 and which alpha-amylase Is modified by having an amino acid deletion of two amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 In SEQ ID NO:1.  
     
     
         79 . The detergent of  claim 78 , wherein the alpha amylase comprises a deletion of three amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:1.  
     
     
         80 . The detergent of  claim 78 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 182 in SEQ ID NO. 1.  
     
     
         81 . The detergent of  claim 78 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 183 and 184 in SEQ ID NO. 1.  
     
     
         82 . The detergent of  claim 78 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 183 in SEQ ID NO. 1.  
     
     
         83 . The detergent of  claim 78 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 182 and 183 in SEQ ID NO. 1.  
     
     
         84 . The detergent of  claim 78 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 182 and 184 in SEQ ID NO. 1.  
     
     
         85 . The detergent of  claim 78 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 184 in SEQ ID NO. 1.  
     
     
         86 . The detergent of  claim 78 , wherein the alpha-amylase comprises an amino acid sequence having at least 85% homology to SEQ ID NO:1.  
     
     
         87 . The detergent of  claim 78 , wherein the alpha-amylase comprises an amino acid sequence having at least 90% homology to SEQ ID NO:1.  
     
     
         88 . A detergent comprising a surfactant and an alpha-amylase, wherein the alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:2 and which alpha-amylase is modified by having an amino acid deletion of two amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:2.  
     
     
         89 . The detergent of  claim 88 , wherein the alpha amylase comprises a deletion of three amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183.184 or 185 in SEQ ID NO:2.  
     
     
         90 . The detergent of  claim 88 , wherein the alpha amylase comprises at least three amino acid deletions of amino acids selected from the group of amino adds equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:2.  
     
     
         91 . The detergent of  claim 88 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 182 in SEQ ID NO. 2.  
     
     
         92 . The detergent of  claim 88 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 183 and 184 in SEQ ID NO. 2.  
     
     
         93 . The detergent of  claim 88 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 183 in SEQ ID NO. 2.  
     
     
         94 . The detergent of  claim 88 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 182 and 183 in SEQ ID. NO. 2.  
     
     
         95 . The detergent of  claim 88 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 182 and 184 in SEQ ID. NO. 2.  
     
     
         96 . The detergent of  claim 88 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 184 in SEQ ID NO. 2.  
     
     
         97 . The detergent of  claim 88 , wherein the alpha-amylase comprising an amino acid sequence having at least 85% homology to SEQ ID NO:2.  
     
     
         98 . The detergent of  claim 88 , wherein the alpha-amylase comprising an amino acid sequence having at least 90% homology to SEQ ID NO:2.  
     
     
         99 . A detergent comprising a surfactant and an alpha-amylase, wherein the alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:3 and which alpha-amylase is modified by having an amino acid deletion of two amino acids selected from the group of amino adds equivalent to positions 178, 179, 180, 181, 182 and 183 in SEQ ID NO:3.  
     
     
         100 . The detergent of  claim 99 , wherein the alpha amylase comprises at least three amino acid deletions of amino acids selected from the group of amino acids equivalent to positions 178, 179, 180, 181, 182 and 183 in SEQ ID NO:3.  
     
     
         101 . The detergent of  claim 99 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 179 and 180 in SEQ ID NO. 3.  
     
     
         102 . The detergent of  claim 99 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 182 in SEQ ID NO. 3.  
     
     
         103 . The detergent of  claim 99 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 179 and 181 in SEQ ID NO. 3.  
     
     
         104 . The detergent of  claim 99 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 180 and 181 in SEQ ID NO. 3.  
     
     
         105 . The detergent of  claim 99 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 180 and 182 in SEQ ID NO. 3.  
     
     
         106 . The detergent of  claim 99 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 179 and 182 in SEQ ID NO. 3.  
     
     
         107 . The detergent of  claim 99 , wherein the alpha-amylase comprises an amino acid sequence having at least 85% homology to SEQ ID NO:3.  
     
     
         108 . The detergent of  claim 99 , wherein the alpha-amylase comprises an amino acid sequence having at least 90% homology to SEQ ID NO:3.  
     
     
         109 . A detergent comprising a surfactant and an alpha-amylase, wherein the alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:7 and which alpha-amylase is modified by having an amino acid deletion of two amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:7.  
     
     
         110 . The detergent of  claim 109 , wherein the alpha amylase comprises at least three amino add deletions of amino acids selected from the group of amino acids equivalent to positions 180, 181, 182, 183, 184 and 185 in SEQ ID NO:7.  
     
     
         111 . The detergent of  claim 109 , wherein the alpha amylase comprises at least three amino acid deletions of amino adds selected from the group of amino acids equivalent to positions 180, 181, 182.183, 184 and 185 in SEQ ID NO:7.  
     
     
         112 . The detergent of  claim 109 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 182 in SEQ ID NO. 7.  
     
     
         113 . The detergent of  claim 109 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 183 and 184 in SEQ ID NO. 7.  
     
     
         114 . The detergent of  claim 109 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 183 in SEQ ID NO. 7.  
     
     
         115 . The detergent of  claim 109 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 182 and 183 in SEQ ID NO. 7.  
     
     
         116 . The detergent of  claim 109 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 182 and 184 in SEQ ID NO. 7.  
     
     
         117 . The detergent of  claim 109 , wherein the alpha-amylase comprises a deletion at positions equivalent to positions 181 and 184 in SEQ ID NO. 7.  
     
     
         118 . The detergent of  claim 109 , wherein the alpha-amylase comprises an amino acid sequence having at least 85% homology to SEQ ID NO:7.  
     
     
         119 . The detergent of  claim 109 , wherein the alpha-amylase comprises an amino add sequence having at least 90% homology to SEQ ID NO:7.

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