Secreted and transmembrane polypeptides and nucleic acids encoding the same

Abstract

The present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

1 - 21 . (Cancelled)  
     
     
         22 . A method for inhibiting new cell growth in a tumor comprising contacting a tissue sample with an isolated polypeptide encoded by a nucleic acid having at least 95% sequence identity to: 
 (a) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42);    (b) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42), lacking its associated signal peptide;    (c) the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41);    (d) the full length coding sequence of the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41); or    (e) the full length coding sequence of the cDNA deposited under ATCC accession number 209492.    
     
     
         23 . A method for inhibiting new cell growth in a tumor comprising contacting a tissue sample with an isolated polypeptide encoded by a nucleic acid having at least 99% sequence identity to: 
 (a) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42);    (b) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42), lacking its associated signal peptide;    (c) the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41);    (d) the full length coding sequence of the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41); or    (e) the full length coding sequence of the cDNA deposited under ATCC accession number 209492.    
     
     
         24 . A method for inhibiting new cell growth in a tumor comprising contacting a tissue sample with a polypeptide encoded by: 
 a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42);    (b) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42), lacking its associated signal peptide;    (c) the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41);    (d) the full length coding sequence of the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41); or    (e) the full length coding sequence of the cDNA deposited under ATCC accession number 209492.    
     
     
         25 . A method for inhibiting new cell growth in a tumor comprising contacting a tissue sample with a polypeptide encoded by a nucleic acid hat hybridizes under high stringency conditions to: 
 (a) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42);    (b) a nucleic acid sequence encoding the polypeptide shown in  FIG. 18  (SEQ ID NO: 42), lacking its associated signal peptide;    (c) the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41);    (d) the full length coding sequence of the nucleic acid sequence shown in  FIG. 17  (SEQ ID NO: 41); or    (e) the full length coding sequence of the cDNA deposited under ATCC accession number 209492.    
     
     
         26 . The method of  claim 25 , wherein hybridization occurs under high stringency conditions comprising 50% formamide, 5×SSC (0.75 M sodium chloride, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5×Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% sodium dodecyl sulphate, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC (0.75 M sodium chloride, 0.075 M sodium citrate) and 50% formamide at 55° C., followed by a high-stringency wash consisting of 0.1×SSC (0.75 M sodium chloride, 0.075 N sodium citrate) containing EDTA at 55° C.