LKB1 gene knockout animals

Abstract

A mammal is provided, in which the LKB1 gene can be deleted phase-specifically and tissue-specifically. These mammals are highly useful as tools to reveal the onset mechanism for diseases caused by LKB1 gene deficiency, such as Peutz-Jeghers syndrome and cancers, as well as to develop therapeutic agents, methods, and so on for the diseases.

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         16 . A transgenic, non-human mammalian cell, in which suppression of the expression of an LKB1 gene can be induced and wherein the cell can be differentiated into an individual mammal.  
     
     
         17 . The cell of  claim 16 , wherein suppression of the expression of the LKB1 gene is induced by deleting at least a part of the LKB1 gene or a regulatory region thereof.  
     
     
         18 . The cell of  claim 16 , wherein at least a part of the LKB1 gene or a regulatory region thereof in the genome of the cell is inserted between a pair of loxP sequences.  
     
     
         19 . The cell of  claim 17 , wherein at least a part of the LKB1 gene or a regulatory region thereof in the genome of the cell is inserted between a pair of loxP sequences.  
     
     
         20 . The cell of  claim 18 , wherein the cell comprises a Cre gene operably linked to a nucleotide sequence that directs expression of the Cre gene.  
     
     
         21 . The cell of  claim 19 , wherein the cell comprises a Cre gene operably linked to a nucleotide sequence that directs expression of the Cre gene.  
     
     
         22 . The cell of  claim 16 , wherein the cell is a rodent cell.  
     
     
         23 . The cell of  claim 18 , wherein the cell is a rodent cell.  
     
     
         24 . The cell of  claim 20 , wherein the cell is a rodent cell.  
     
     
         25 . The cell of  claim 22 , wherein the cell is a mouse cell.  
     
     
         26 . The cell of  claim 23 , wherein the cell is a mouse cell.  
     
     
         27 . The cell of  claim 24 , wherein the cell is a mouse cell.  
     
     
         28 . The cell of  claim 16 , wherein the cell is an embryonic stem cell.  
     
     
         29 . The cell of  claim 17 , wherein the cell is an embryonic stem cell.  
     
     
         30 . The cell of  claim 18 , wherein the cell is an embryonic stem cell.  
     
     
         31 . The cell of  claim 20 , wherein the cell is an embryonic stem cell.  
     
     
         32 . The cell of  claim 22 , wherein the cell is an embryonic stem cell.  
     
     
         33 . The cell of  claim 25 , wherein the cell is an embryonic stem cell.  
     
     
         34 . A transgenic, non-human mammalian cell, in which the expression of an LKB1 gene is inducibly suppressed and wherein the cell can be differentiated into an individual mammal.  
     
     
         35 . The cell of  claim 34 , wherein the expression of the LKB1 gene is suppressed by a defect in at least a part of the LKB1 gene or a regulatory region thereof.  
     
     
         36 . The cell of  claim 16 , wherein at least a part of the LKB1 gene or a regulatory region thereof in the genome of the cell is inserted between a pair of loxP sequences.  
     
     
         37 . A transgenic, non-human mammalian cell, produced by the process of expressing a Cre gene in the cell of  claim 18 .  
     
     
         38 . The cell of  claim 34 , wherein the cell is a rodent cell.  
     
     
         39 . The cell of  claim 35 , wherein the cell is a rodent cell.  
     
     
         40 . The cell of  claim 36 , wherein the cell is a rodent cell.  
     
     
         41 . The cell of  claim 37 , wherein the cell is a rodent cell.  
     
     
         42 . The cell of  claim 38 , wherein the rodent cell is a mouse cell.  
     
     
         43 . The cell of  claim 39 , wherein the rodent cell is a mouse cell.  
     
     
         44 . The cell of  claim 40 , wherein the rodent cell is a mouse cell.  
     
     
         45 . The cell of  claim 41 , wherein the rodent cell is a mouse cell.  
     
     
         46 . The cell of  claim 34 , wherein the cell is an embryonic stem cell.  
     
     
         47 . The cell of  claim 35 , wherein the cell is an embryonic stem cell.  
     
     
         48 . The cell of  claim 36 , wherein the cell is an embryonic stem cell.  
     
     
         49 . The cell of  claim 37 , wherein the cell is an embryonic stem cell.  
     
     
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