Novel guanosine monophosphate reductase

Abstract

The present invention provides a human guanosine monophosphate reductase (HGMPR) and polynucleotides which identify and encode HGMPR. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding HGMPR and a method for producing HGMPR. The invention also provides for agonists, antibodies, or antagonists specifically binding HGMPR, and their use, in the prevention and treatment of diseases associated with expression of HGMPR. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding HGMPR for the treatment of diseases associated with the expression of HGMPR. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding HGMPR.

1 . A substantially purified guanosine monophosphate reductase comprising the amino acid sequence of SEQ ID NO:1 or, fragments thereof.  
     
     
         2 . An isolated and purified polynucleotide sequence encoding the guanosine monophosphate reductase of  claim 1 .  
     
     
         3 . A polynucleotide sequence which hybridizes under stringent conditions to the polynucleotide sequence of  claim 2 .  
     
     
         4 . A hybridization probe comprising the polynucleotide sequence of  claim 2 .  
     
     
         5 . An isolated and purified polynucleotide sequence comprising SEQ ID NO:2 or variants thereof.  
     
     
         6 . A polynucleotide sequence which is complementary to the polynucleotide sequence of  claim 2  or variants thereof.  
     
     
         7 . A hybridization probe comprising the polynucleotide sequence of  claim 6 .  
     
     
         8 . An expression vector containing the polynucleotide sequence of  claim 2 .  
     
     
         9 . A host cell containing the vector of  claim 8 .  
     
     
         10 . A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:1 the method comprising the steps of: 
 a) culturing the host cell of  claim 9  under conditions suitable for the expression of the polypeptide; and    b) recovering the polypeptide from the host cell culture.    
     
     
         11 . A pharmaceutical composition comprising a substantially purified guanosine monophosphate reductase having the amino acid sequence of  claim 1  in conjunction with a suitable pharmaceutical carrier.  
     
     
         12 . A purified antibody which binds specifically to the polypeptide of  claim 1 .  
     
     
         13 . A purified agonist which specifically binds to and modulates the activity of the polypeptide of  claim 1 .  
     
     
         14 . A purified antagonist which specifically binds to and modulates the activity of the polypeptide of  claim 1 .  
     
     
         15 . A pharmaceutical composition comprising the purified antagonist of  claim 14  in conjunction with a suitable pharmaceutical carrier.  
     
     
         16 . A method for treating cancer comprising administering to a subject in need of such treatment an effective amount of the pharmaceutical composition of  claim 15 .  
     
     
         17 . A method for treating a viral disease comprising administering to a subject in need of such treatment an effective amount of the pharmaceutical composition of  claim 15 .  
     
     
         18 . A method for treating an inflammatory disease comprising administering to a subject in need of such treatment an effective amount of the pharmaceutical composition of  claim 15 .  
     
     
         19 . A method for treating an immunological disorder comprising administering to subject in need of such treatment an effective amount of the pharmaceutical composition of  claim 15 .  
     
     
         20 . A method for detection of polynucleotides encoding guanosine monophosphate reductase in a biological sample comprising the steps of: 
 a) hybridizing the polynucleotide of  claim 6  to nucleic acid material of a biological sample, thereby forming a hybridization complex; and    b) detecting said hybridization complex, wherein the presence of said complex correlates with the presence of a polynucleotide encoding guanosine monophosphate reductase in said biological sample.