Charged component detector, its using method and detection panel

Abstract

The present invention provides a charged substance detector capable of detecting charged substances easily and quickly without complicating the construction of the detector, a method of using the charged substance detector and a detection panel. The charged substance detector comprises: a detection panel 1 having a plurality of reaction electrodes 2 arrayed in matrix, on which are immobilized reaction charged substances reacting specifically with target charged substances; and a conduction control device 4 having a matrix of wires 3 crossing each other at points corresponding to the matrix-arrayed reaction electrodes 2 of the detection panel 1 , and being capable of selectively energizing the reaction electrodes 2 through the matrix of wires 3 . Further, to detect a state of reaction in each of the reaction electrodes 2 , the conduction control device 4 is provided with a conduction state detector 5 . By selectively applying a voltage to a specific reaction electrode 2 , the reaction charged substance immobilized on the reaction electrode 2 is allowed to react specifically with a target charged substance. Further, the present invention also covers the detection panel 1 constituting the charged substance detector.

1 . A charged substance detector comprising: 
 a detection panel having a plurality of reaction electrodes arrayed in matrix, on which are immobilized reaction charged substances reacting specifically with target charged substances; and    a conduction control device which has a matrix of wires crossing each other at points corresponding to the matrix-arrayed reaction electrodes of the detection panel, and which can selectively energize the reaction electrodes through the matrix of wires.    
     
     
         2 . The charged substance detector according to  claim 1 , wherein the target charged substance is DNA or gene.  
     
     
         3 . The charged substance detector according to  claim 1 , wherein the detection panel comprises a first board having the plurality of reaction electrodes arrayed in matrix on which the reaction charged substances that specifically react with the target charged substances, and the second board opposing the first board and having an electrode to apply a voltage between it and the selected reaction electrode in the matrix.  
     
     
         4 . The charged substance detector according to  claim 3 , wherein the second board has a plurality of electrodes arranged at positions corresponding to the matrix-arrayed reaction electrodes of the first board.  
     
     
         5 . The charged substance detector according to  claim 1 , wherein the reaction electrodes, on which are immobilized the reaction charged substances that specifically react with the target charged substances, has an immobilizing layer for the reaction charged substances.  
     
     
         6 . The charged substance detector according to  claim 1 , wherein the conduction control device has switch elements connected between the plurality of reaction electrodes arrayed in matrix and the matrix of wires and selectively turns on or off the switch elements to apply a predetermined voltage to a specific reaction electrode.  
     
     
         7 . The charged substance detector according to  claim 1 , wherein the conduction control device has a conduction state detector for detecting a conduction state of each reaction electrode.  
     
     
         8 . A method of using the charged substance detector according to  claim 1 , comprising the step of: 
 applying a voltage to a specific reaction electrode of the detection panel to immobilize a predetermined reaction charged substance to the specified reaction electrode.    
     
     
         9 . A method of using the charged substance detector according to  claim 1 , comprising the steps of: 
 to determine whether the same target charged substance is present in a plurality of samples, immobilizing a predetermined reaction charged substance on a required number of reaction electrodes of the detection panel; and    successively applying a predetermined voltage to specific reaction electrodes corresponding to each of the different samples to successively allow the predetermined reaction charged substance to react specifically with the target charged substance in each sample.    
     
     
         10 . A method of using the charged substance detector according to  claim 1 , comprising the steps of: 
 to determine whether a plurality of target charged substances are present in one sample, successively immobilizing different reaction charged substances to each of specific reaction electrodes of the detection panel; and    applying a predetermined voltage to each of the reaction electrodes to successively allow the different reaction charged substances to react specifically with the corresponding target charged substances in the sample.    
     
     
         11 . A method of using the charged substance detector according to  claim 1 , comprising the steps of: 
 to determine whether a plurality of target charged substances are present in a plurality of samples, successively immobilizing different reaction charged substances to each of reaction electrodes of the detection panel; and    applying a predetermined voltage to each of the reaction electrodes corresponding to each of the samples to successively allow the reaction charged substances on the reaction electrodes to react specifically with the corresponding target charged substances in the samples.    
     
     
         12 . A detection panel used in the charged substance detector according to  claim 1 , wherein the reaction charged substances the number of which is equal to that of inspection items are assigned and immobilized to predetermined addresses of the reaction electrodes.