Screening for novel bioactivities

Abstract

Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing an assay system. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.

1 . A method for identifying a desired activity encoded by a genomic DNA population comprising: 
 (a) obtaining a single-stranded genomic DNA population;    (b) contacting the single-stranded DNA population of (a) with a DNA probe bound to a ligand under conditions and for sufficient time to allow hybridization and to produce a double-stranded complex of probe and members of the genomic DNA population which hybridize thereto;    (c) contacting the double-stranded complex of (b) with a solid phase specific binding partner for said ligand so as to produce a solid phase complex;    (d) separating the solid phase complex from the single-stranded DNA population of (b);    (e) releasing from the probe the members of the genomic population which had bound to the solid phase bound probe;    (f) forming double-stranded DNA from the members of the genomic population of (e);    (g) introducing the double-stranded DNA of (f) into a suitable host cell to produce an expression library containing a plurality of clones containing the selected DNA; and    (h) screening the expression library for the desired activity.    
     
     
         2 . The method of  claim 1 , wherein the genomic DNA population is derived from uncultivated or cultivated microorganisms.  
     
     
         3 . The method of  claim 2 , wherein the uncultivated or cultivated microorganisms are isolated from an environmental sample.  
     
     
         4 . The method of  claim 3 , wherein the microorganisms isolated from an environmental sample are extremophiles.  
     
     
         5 . The method of  claim 4 , wherein the extremophiles are selected from the group consisting of thermophiles, hyperthermophiles, psychrophiles, halophiles, acidophiles, barophiles and psychrotrophs.  
     
     
         6 . The method of  claim 1 , wherein the genomic DNA, or fragments thereof, comprise one or more operons, or portions thereof.  
     
     
         7 . The method of  claim 6 , wherein the operons, or portions thereof, encodes a complete or partial metabolic pathway.  
     
     
         8 . The method of  claim 7 , wherein the operons or portions thereof encoding a complete or partial metabolic pathway encodes polyketide synthases.  
     
     
         9 . The method of  claim 1 , wherein the expression library containing a plurality of clones is selected from the group consisting of phage, plasmids, phagemids, cosmids, phosmids, viral vectors and artificial chromosomes.  
     
     
         10 . The method of  claim 1 , wherein the a suitable host cell is selected from the group consisting of a bacterium, fungus, plant cell, insect cell and animal cell.  
     
     
         11 . The method of  claim 1 , wherein the DNA probe bound to a ligand is comprised of at least a portion of the coding region sequence of DNA for a known bioactivity.  
     
     
         12 . The method of  claim 1 , wherein the ligand is selected from the group consisting of antigens or haptens, biotin or iminobiotin, sugars, enzymes, apoenzymes homopolymeric oligonucleotides and hormones.  
     
     
         13 . The method of  claim 1 , wherein the binding partner for said ligand is selected from the group consisting of antibodies or specific binding fragments thereof, avidin or streptavidin, lectins, enzyme inhibitors, apoenzyme cofactors, homopolymeric oligonucleotides and hormone receptors.  
     
     
         14 . The method of  claim 1 , wherein a solid phase is selected from the group consisting of a glass or polymeric surface, a packed column of polymeric beads or magnetic or paramagnetic particles.  
     
     
         15 . The method of  claim 1 , further comprising producing an extract of the expression library.  
     
     
         16 . The method of  claim 15 , further comprising combining the expression library extract with an enzyme extract from a metabolically rich host organism.  
     
     
         17 . The method of  claim 16 , wherein the host organism is  Streptomyces.    
     
     
         18 . The method of  claim 16 , wherein the host organism is  Bacillus.    
     
     
         19 . A method for preselecting a desired DNA from a genomic DNA population comprising: 
 (a) obtaining a single-stranded genomic DNA population;    (b) contacting the single-stranded DNA population of (a) with a ligand-bound oligonucleotide probe that is complementary to a secretion signal sequence unique to a given class of proteins under conditions permissive of hybridization to form a double-stranded complex;    (c) contacting the double-stranded complex of (a) with a solid phase specific binding partner for said ligand so as to produce a solid phase complex;    (d) separating the solid phase complex from the single-stranded DNA population of (a);    (e) releasing the members of the genomic population which had bound to said solid phase bound probe;    (f) separating the solid phase bound probe from the members of the genomic population which had bound thereto;    (g) forming double-stranded DNA from the members of the genomic population of (e);    (h) introducing the double-stranded DNA of (g) into a suitable host cell to form an expression library containing a plurality of clones containing the selected DNA; and    (i) screening the expression library for the desired activity.    
     
     
         20 . The method of  claim 19 , wherein the genomic DNA population is derived from uncultivated or cultivated microorganisms.  
     
     
         21 . The method of  claim 20 , wherein the uncultivated or cultivated microorganisms are isolated from an environmental sample.  
     
     
         22 . The method of  claim 21 , wherein the microorganisms isolated from an environmental sample are extremophiles.  
     
     
         23 . The method of  claim 22 , wherein the extremophiles are selected from the group consisting of thermophiles, hyperthermophiles, psychrophiles, halophiles, acidophiles, barophiles and psychrotrophs.  
     
     
         24 . The method of  claim 19 , wherein the genomic DNA, or fragments thereof, comprise one or more operons, or portions thereof.  
     
     
         25 . The method of  claim 24 , wherein the operons, or portions thereof, encodes a complete or partial metabolic pathway.  
     
     
         26 . The method of  claim 25 , wherein the operons or portions thereof encoding a complete or partial metabolic pathway encodes polyketide synthases.  
     
     
         27 . The method of  claim 19 , wherein the expression library containing a plurality of clones is selected from the group consisting of phage, plasmids, phagemids, cosmids, phosmids, viral vectors and artificial chromosomes.  
     
     
         28 . The method of  claim 19 , wherein the a suitable host cell is selected from the group consisting of a bacterium, fungus, plant cell, insect cell and animal cell.  
     
     
         29 . The method of  claim 19 , wherein the DNA probe bound to a ligand is comprised of at least a portion of the coding region sequence of DNA for a known bioactivity.  
     
     
         30 . The method of  claim 19 , wherein the ligand is selected from the group consisting of antigens or haptens, biotin or iminobiotin, sugars, enzymes, apoenzymes homopolymeric oligonucleotides and hormones.  
     
     
         31 . The method of  claim 19 , wherein the binding partner for said ligand is selected from the group consisting of antibodies or specific binding fragments thereof, avidin or streptavidin, lectins, enzyme inhibitors, apoenzyme cofactors, homopolymeric oligonucleotides and hormone receptors.  
     
     
         32 . The method of  claim 19 , wherein a solid phase is selected from the group consisting of a glass or polymeric surface, a packed column of polymeric beads or magnetic or paramagnetic particles.  
     
     
         33 . The method of  claim 19 , further comprising producing an extract of the expression library.  
     
     
         34 . The method of  claim 33 , further comprising combining the expression library extract with an enzyme extract from a metabolically rich host organism.  
     
     
         35 . The method of  claim 34 , wherein the host organism is  Streptomyces.    
     
     
         36 . The method of  claim 34 , wherein the host organism is  Bacillus.