Thermostable DNA polymerase from anaerocellum thermophilum

Abstract

A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophilum. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.

1 - 21 . (Cancelled)  
     
     
         22 . An isolated DNA encoding a purified thermostable DNA polymerase obtainable from  Anaerocellum thermophilum  that catalyses the template directed polymerisation of DNA, possesses 5′-3′polymerase activity, lacks 3′-5′-exonuclease activity, has a magnesium-dependent reverse transcriptase activity, and has a manganese-dependent reverse transcriptase activity.  
     
     
         23 . The isolated DNA sequence of  claim 22  that has the nucleotide sequence shown in SEQ ID NO. 7.  
     
     
         24 . A vector containing the isolated DNA sequence of  claim 23 .  
     
     
         25 . A microbial host transformed with the vector of claim  26 .