EB matrix production from fetal tissues and its use for tissue repair

Abstract

A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.

1 . A method for producing and preserving a biopolymer scaffold material, comprising the steps of: 
 a. harvesting tissue from an animal source;    b. inactivating infective agents of said tissue;    c. applying pressure to said tissue to remove undesirable components from said tissue;    d. delipidizing said tissue; and    e. washing said tissue to remove chemical residues    
     
     
         2 . The method of  claim 1  wherein said tissue is selected from the group consisting of fetal, neo-natal and post-natal animal tissue.  
     
     
         3 . The method of  claim 2  wherein said tissue is bovine.  
     
     
         4 . The method of  claim 2  wherein said tissue is porcine.  
     
     
         5 . A method for using the biopolymer scaffold material produced in  claim 1  by applying said biopolymer scaffold material to a lesion or to damaged tissue to promote tissue regeneration.  
     
     
         6 . The method of  claim 1  further comprising: 
 f. combining said biopolymer scaffold material with with signaling complexes OF stem cells, or with drugs.    
     
     
         7 . A method for using the biopolymer scaffold material as in  claim 1  for hernia repair, the method comprising applying said biopolymer scaffold material to repair a hernia.  
     
     
         8 . A method for using the biopolymer scaffold material as in  claim 1  for colon, rectal, vaginal or urethral prolapse treatment, the method comprising applying said biopolymer scaffold tissue to repair a colon, rectal, or urethral prolapse.  
     
     
         9 . A method for using the biopolymer scaffold material as in  claim 1  for pelvic floor reconstruction, the method comprising applying said biopolymer scaffold tissue to reconstruct a pelvic floor.  
     
     
         10 . A method for using the biopolymer scaffold material as in  claim 1  for muscle flap reinforcement, the method comprising applying said biopolymer scaffold tissue to reinforce a muscle flap.  
     
     
         11 . A method for using the biopolymer scaffold material produced as in  claim 1  for supporting soft tissue of the lung the method comprising applying said biopolymer scaffold tissue to provide a soft tissue support for a lung.  
     
     
         12 . A method for using the biopolymer scaffold material produced as in  claim 1  for rotator cuff repair and replacement, the method comprising applying said biopolymer scaffold tissue to repair or replace a rotator cuff.  
     
     
         13 . A method for using the biopolymer scaffold material produced as in  claim 1  for periosteum replacement, the method comprising applying said biopolymer scaffold tissue to replace periosteum.  
     
     
         14 . A method for using the biopolymer scaffold material produced as in  claim 1  for dura repair, the method comprising applying said biopolymer scaffold tissue to repair dura.  
     
     
         15 . A method for using the biopolymer scaffold material produced as in  claim 1  for pericardial membrane repair, the method comprising applying said biopolymer scaffold tissue to repair a pericardial membrane.  
     
     
         16 . A method for using the biopolymer scaffold material produced as in  claim 5  for soft tissue augmentation the method comprising applying said biopolymer scaffold tissue to repair or replace a rotator cuff.  
     
     
         17 . A method for using the biopolymer scaffold material as in  claim 1  for intervertebral disk repair, the method comprising applying said biopolymer scaffold tissue to repair a intervertebral disk.  
     
     
         18 . A method for using the biopolymer scaffold material as in  claim 1  for periodontal repair, the method comprising applying said biopolymer scaffold tissue to repair periodontal tissue.  
     
     
         19 . A method for using the biopolymer scaffold material as in  claim 1  to provide a urethral sling the method comprising applying said biopolymer tissue to provide a urethral sling.  
     
     
         20 . A method for using the biopolymer scaffold material produced in  claim 1  to provide a laminectomy barrier the method comprising applying said biopolymer scaffold tissue to provide a laminectomy barrier.  
     
     
         21 - 31 . (Canceled).