Gene regulating aureobasidin sensitivity

Abstract

The invention is directed to isolated DNAs having nucleic acid sequences which encode proteins which regulate aureobasidin sensitivity. Also disclosed are recombinant plasmids containing the DNAs, transformants containing the plasmids, and methods of producing the proteins.

1 . A method for cloning a gene regulating aureobasidin sensitivity which is obtained from a mold and encodes a protein regulating aureobasidin sensitivity or its functional derivative, which comprises using a gene or fragment thereof contained in a DNA fragment represented by a restriction enzyme map of FIG. 1 to FIG. 3 as a probe.  
     
     
         2 . A nucleic acid probe which comprises a sequence consisting of at least 15 bases and is hybridizable with a gene regulating aureobasidin sensitivity which is obtained from a mold and encodes a protein regulating aureobasidin sensitivity on its functional derivative.  
     
     
         3 . A method for detecting a gene regulating aureobasidin sensitivity by hybridization with the use of a nucleic acid probe as claimed in  claim 2 .  
     
     
         4 . A process for cloning a gene coding for a protein which regulates aureobasidin sensitivity, which comprises using a gene or fragment thereof contained in a DNA fragment represented by a restriction enzyme map of FIG. 1 to FIG. 3 as a probe.  
     
     
         5 . A nucleic acid probe which comprises a sequence consisting of 15 or more bases and is hybridizable with a gene encoding a protein regulating aureobasidin sensitivity.  
     
     
         6 . A process for screening an aureobasidin resistant gene comprising: 
 (a) mutanizing an aureobasidin sensitive host, to thereby give a resistant mutant; and    (b) isolating a gene capable of conferring aureobasidin resistance from said resistant mutant.    
     
     
         7 . A process for screening an aureobasidin sensitive gene comprising: 
 (a) mutanizing an aureobasidin sensitive host, to thereby give a resistant mutant;    (b) isolating a gene capable of conferring aureobasidin resistance from said resistant mutant; and    (c) isolating aureobasidin sensitive gene from said aureobasidin sensitive host of step (a), which is hybridizable with said gene capable of conferring aureobasidin resistance of step (b).