Human type V adenylyl cyclase

Abstract

A DNA sequence encoding a human adenylyl cyclase is described. The amino acid sequence of the adenylyl cyclase is also described.

1 - 7 . (Canceled).  
     
     
         8 . A method for identifying a candidate compound which binds to a polypeptide selected from the group consisting of: 
 a) a polypeptide which is at least 95% identical to the amino acid sequence of SEQ ID NO:2;    b) a polypeptide which is at least 95% identical to the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-4115; and    c) a polypeptide encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO:1;    wherein the polypeptide has adenylyl cyclase activity; the method comprising:    i) contacting a sample comprising the polypeptide with a test compound under conditions suitable for binding; and    ii) detecting binding of the test compound to the polypeptide;    thereby identifying a compound which binds to the polypeptide.    
     
     
         9 . The method of  claim 8 , wherein the sample is an isolated polypeptide, a membrane-bound form of an isolated polypeptide or a cell comprising the polypeptide.  
     
     
         10 . The method of  claim 9 , wherein the cell is a mammalian cell.  
     
     
         11 . The method of  claim 8 , wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of: 
 a) direct detection of test compound/polypeptide binding;    b) a competition binding assay; and    c) an immunoassay.    
     
     
         12 . The method of  claim 8 , wherein the binding of the test compound to the polypeptide is detected is by an assay for an activity of the polypeptide.  
     
     
         13 . The method of  claim 12 , wherein the assay for activity is selected from the group consisting of: 
 a) an assay for measuring adenylyl cyclase activity; and    b) an assay for measuring the synthesis of cAMP.    
     
     
         14 . A method for identifying a candidate compound which binds to a polypeptide selected from the group consisting of: 
 a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2;    b) a polypeptide comprising the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-4115; and    c) a polypeptide encoded by the nucleotide sequence set forth in SEQ ID NO: 1; the method comprising:    i) contacting a sample comprising the polypeptide with a test compound under conditions suitable for binding; and    ii) detecting binding of the test compound to the polypeptide;    thereby identifying a compound which binds to the polypeptide.    
     
     
         15 . The method of  claim 14 , wherein the sample is an isolated polypeptide, a membrane-bound form of an isolated polypeptide or a cell comprising the polypeptide.  
     
     
         16 . The method of  claim 15 , wherein the cell is a mammalian cell.  
     
     
         17 . The method of  claim 14 , wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of: 
 a) direct detection of test compound/polypeptide binding;    b) a competition binding assay; and    c) an immunoassay.    
     
     
         18 . The method of  claim 14 , wherein the binding of the test compound to the polypeptide is detected is by an assay for an activity of the polypeptide.  
     
     
         19 . The method of  claim 18 , wherein the assay for activity is selected from the group consisting of: 
 a) an assay for measuring adenylyl cyclase activity; and    b) an assay for measuring the synthesis of cAMP.    
     
     
         20 . A method for identifying a candidate compound which binds to a polypeptide selected from the group consisting of: 
 a) a polypeptide comprising a fragment of at least 15 contiguous amino acids of SEQ ID NO:2;    b) a polypeptide comprising a fragment of at least 15 contiguous amino acids of the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-4115; and    c) a polypeptide comprising a fragment of at least 15 contiguous amino acids encoded by the nucleotide sequence set forth in SEQ ID NO:1;    wherein the polypeptide has adenylyl cyclase activity; the method comprising:    i) contacting a sample comprising the polypeptide with a test compound under conditions suitable for binding; and    ii) detecting binding of the test compound to the polypeptide;    thereby identifying a compound which binds to the polypeptide.    
     
     
         21 . The method of  claim 20 , wherein the sample is an isolated polypeptide, a membrane-bound form of an isolated polypeptide or a cell comprising the polypeptide.  
     
     
         22 . The method of  claim 21 , wherein the cell is a mammalian cell.  
     
     
         23 . The method of  claim 20 , wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of: 
 a) direct detection of test compound/polypeptide binding;    b) a competition binding assay; and    c) an immunoassay.    
     
     
         24 . The method of  claim 20 , wherein the binding of the test compound to the polypeptide is detected is by an assay for an activity of the polypeptide.  
     
     
         25 . The method of  claim 24 , wherein the assay for activity is selected from the group consisting of: 
 a) an assay for measuring adenylyl cyclase activity; and    b) an assay for measuring the synthesis of cAMP.