ACPL DNA and polypeptides

Abstract

The invention is directed to purified and isolated novel ACPL polypeptides, the nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, fragmented peptides derived from these polypeptides, and the uses of the above.

1 - 17 . Canceled.  
     
     
         18 . An isolated nucleic acid molecule comprising the coding region of SEQ ID NO:1.  
     
     
         19 . An isolated nucleic acid molecule comprising the complement of DNA capable of hybridizing under moderately stringent conditions to the DNA of SEQ ID NO:1 wherein the moderately stringent condition include 50% formamide and 6×SSC, at 42° C. with washing conditions of 60° C., 0.5×SSC, 0.1% SDS, and, wherein the DNA encodes a polypeptide that is a cofactor in IL-18 binding.  
     
     
         20 . An isolated nucleic acid molecule encoding a polypeptide, wherein said polypeptide comprises an amino acid sequence that is at least 80% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:2.  
     
     
         21 . An isolated nucleic acid molecule comprising a DNA encoding a polypeptide comprising the amino acids of SEQ ID NO:2.  
     
     
         22 . An isolated nucleic acid comprising a DNA encoding a polypeptide, wherein said polypeptide comprises an amino acid sequence that is at least 80% identical to amino acids x, to 356 of SEQ ID NO:2, wherein x, is amino acid 1 or 15.  
     
     
         23 . An isolated nucleic acid comprising a DNA encoding a soluble polypeptide comprising amino acids x, to 356 of SEQ ID NO:2, wherein x, is amino acid 1 or 15.  
     
     
         24 . An isolated nucleic acid comprising a DNA that encodes a polypeptide comprising a fragment of SEQ ID NO:2, wherein the fragment is a cofactor in IL-18 binding.  
     
     
         25 . An isolated nucleic acid comprising DNA that encodes a polypeptide comprising a fragment that is at least 80% identical to SEQ ID NO:2, wherein the fragment is a cofactor in IL-18 binding.  
     
     
         26 . An isolated polypeptide comprising an amino acid selected from the group consisting of 
 (a) SEQ ID NO:2.    (b) fragments of SEQ ID NO:2, wherein the fragment is a cofactor in IL-18 binding.    
     
     
         27 . A isolated polypeptide comprising a polypeptide encoded by DNA selected from the group consisting of: 
 (a) the coding region of SEQ ID NO:1;    (b) the complement of DNA capable of hybridizing under moderately stringent conditions to the DNA of SEQ ID NO:1, wherein the moderately stringent condition include 50% formamide and 6×SSC, at 42° C. with washing conditions of 60° C., 0.5×SSC, 0.1% SDS, and, wherein the DNA encodes a polypeptide that is a cofactor in IL-18 binding; and    (c) DNA that is degenerate to the DNA of SEQ ID NO:1.    
     
     
         28 . An isolated polypeptide comprising an amino acid sequence that is at least 80% identical to a sequence selected from the group consisting of: 
 (a) amino acids x 1  to 356 of SEQ ID NO:2, wherein x 1  is amino acid 1 or 15; and    (b) fragments of SEQ ID NO:2 wherein the fragment is a cofactor in IL-18 binding.    
     
     
         29 . A polypeptide comprising an amino acid sequence selected from the group consisting of: 
 (a) amino acids x 1  to 356 of SEQ ID NO:2, wherein x 1  is amino acid 1 or 15; and    (b) fragments of SEQ ID NO:2 wherein the fragment is a cofactor in IL-18 binding.    
     
     
         30 . A fusion protein comprising the Fc region of Ig a polypeptide selected from the group consisting of: 
 (a) amino acids x1 to 356 of SEQ ID NO:2, wherein x1 is amino acid 1 or 15; and    (b) a fragment of (a), wherein the fragment is a cofactor in IL-18 binding.    (c A polypeptide that is at least 80% identical to (a), wherein the polypeptide is a cofactor in IL-18 binding.    
     
     
         31 . A recombinant expression vector comprising a DNA selected from the group consisting of: 
 (a) the coding region of SEQ ID NO:1;    (b) a DNA encoding amino acids x1 to 356 of SEQ ID NO:2, wherein x1 is amino acid 1 or 15; and    (c a DNA encoding an polypeptide that is at least 80% identical to the polypeptide of (b).    
     
     
         32 . A process for preparing a polypeptide, the process comprising culturing a host cell transformed with an expression vector of  claim 31  under conditions that promote expression of the polypeptide.  
     
     
         33 . A host cell transformed or transfected with an expression vector according to  claim 32 .  
     
     
         34 . An antibody that is immunoreactive with a polypeptide of  claim 26.