Device for producing polymer support materials in the form of porous polymer beads

Abstract

The invention relates to a process for synthesis, by inverse bead polymerization of a monomer phase, of a bead-like, cross-linked, hydrophilic copolymer which has binding activity toward ligands containing nucleophilic groups. The invention relates to support polymer materials with high binding capacity for penicillin acylase and low swelling factor, as well as to use of the same.

1 - 10 . (Canceled).  
     
     
         11 . A support polymer material, having a binding capacity for penicillin amidase from  E.  coli of at least 220 U/g moist, based on a reaction of 1530 units of penicillin amidase with 1 g of said support polymer material, and 
 said support polymer having a swelling factor of at most 1.5.    
     
     
         12 . A support polymer material, having a binding capacity for penicillin amidase from  E. coli  of at least 220 U/g moist, based on a reaction of 1530 units of penicillin amidase with 1 g of said support polymer material, and 
 said support polymer having a swelling factor of at most 1.5;    said support polymer being obtained from the following monomers a), b), and c):    a) 5 to 40 wt % of hydrophilic monomers which contain a vinyl group, said hydrophilic monomers being capable of radical polymerization, and being capable of forming at least 10% aqueous solutions at room temperature,    b) 30 to 50 wt % of monomers which contain a vinyl group and an additional functional group, said monomers being capable of radical polymerization and being capable of forming at least one covalent bond in a reaction with at least one nucleophilic group of a ligand, and    c) 20 to 60 wt % of cross-linking monomers which contain two or more ethylenically unsaturated polymerizable groups, said cross-linking monomers being capable of radical polymerization,    wherein a), b) and c) add up to 100 wt %.    
     
     
         13 . The support polymer material according to  claim 12 , wherein said monomers are 
 a) acrylamide, methacrylamide or mixtures thereof,    b) glycidyl methacrylate, allyl glycidyl ether or mixtures thereof,    c) methylenebisacrylamide or methylenebismethacrylamide.    
     
     
         14 . A method of binding proteins, comprising: 
 contacting the support polymer material according to  claim 11  with at least one protein.    
     
     
         15 . A method of binding enzymes, comprising: 
 contacting the support polymer material according to  claim 11  with at least one enzyme.    
     
     
         16 . A method of binding antibodies, comprising: 
 contacting the support polymer material according to  claim 11  with at least one antibody.    
     
     
         17 . A method of chromatography, comprising: 
 contacting the support polymer material according to  claim 11  with at least one compound.    
     
     
         18 . A method for synthesis of pharmaceuticals, comprising: 
 synthezising a pharmaceutical in the presence of the support polymer material according to  claim 11 .    
     
     
         19 . A method for stereospecific synthesis of chiral substances, comprising: 
 synthezising a chiral substance in the presence of the support polymer material according to  claim 11 .    
     
     
         20 . The support polymer material according to  claim 12 , wherein said monomer a) is a methacrylamide.  
     
     
         21 . The support polymer material according to  claim 12 , wherein said functional group of monomer b) is an oxirane group.  
     
     
         22 . The support polymer material according to  claim 12 , wherein said ligand of said nucleophilic group is an oxirane group.  
     
     
         23 . The support polymer material according to  claim 12 , wherein said monomer c) is N,N′-methylenebismethacrylamide.  
     
     
         24 . A method of covalently binding of a ligand, comprising: 
 contacting the support polymer material according to  claim 11  with a ligand to covalently bind the ligand to the support polymer material;    wherein said support polymer material has an oxirane group.    
     
     
         25 . A method of binding proteins, comprising: 
 contacting the support polymer material according to  claim 12  with at least one protein.    
     
     
         26 . A method of binding enzymes, comprising: 
 contacting the support polymer material according to  claim 12  with at least one enzyme.    
     
     
         27 . A method of binding antibodies, comprising: 
 contacting the support polymer material according to  claim 12  with at least one antibody.    
     
     
         28 . A method of chromatography, comprising: 
 contacting the support polymer material according to  claim 12  with at least one compound.    
     
     
         29 . A method for synthesis of pharmaceuticals, comprising: 
 synthezising a pharmaceutical in the presence of the support polymer material according to  claim 12 .    
     
     
         30 . A method for stereospecific synthesis of chiral substances, comprising: 
 synthezising a chiral substance in the presence of the support polymer material according to  claim 12 .    
     
     
         31 . A method of covalently binding of a ligand, comprising: 
 contacting the support polymer material according to  claim 12  with a ligand to covalently bind the ligand to the support polymer material;    wherein said support polymer material has an oxirane group.