Diagnostic drugs for autoimmune diseases

Abstract

A diagnostic drug and a diagnostic kit for autoimmune diseases including at least one of a polypeptide selected from an HMG-1 family, a polypeptide selected from an HMG-2 family, a fragment thereof which is reactable with an antibody of an autoimmune disease patient, and a method for detecting an antibody of an autoimmune disease patient using the same are provided.

1 .- 9 . (canceled)  
     
     
         10 . A method for diagnosing an autoimmune disease in a patient, the method comprising the step of detecting one or more antibodies in the patient by contacting a reagent with antibodies from the patient; wherein: 
 the reagent comprises at least one antigen selected from the group consisting of a first polypeptide, a second polypeptide, a fragment of the first polypeptide, and a fragment of the second polypeptide; the first polypeptide having an amino acid sequence indicated by SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5; and the second polypeptide having an amino acid sequence indicated by SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:11;    the at least one antigen specifically binds with an antibody from an autoimmune disease patient; and    the autoimmune disease is selected from the group consisting of rheumatoid arthritis, human systemic lupus erythematosus, Sjögren's syndrome, Beh et's disease, primary biliary cirrhosis, microscopic polyangitis/polyarteritis nodosa, ulcerative colitis, Crohn's disease, and autoimmune hepatitis.    
     
     
         11 . A method for diagnosing an autoimmune disease in a patient, the method comprising the step of detecting one or more antibodies in the patient by contacting a reagent with antibodies from the patient, the reagent comprising a mixture of a first antigen and a second antigen; wherein: 
 the first antigen is selected from the group consisting of a polypeptide having an amino acid indicated by SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, or a fragment thereof, the polypeptide or fragment specifically binds with an antibody from an autoimmune disease patient;    the second antigen is selected from the group consisting of a polypeptide having an amino acid sequence indicated by SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:1O, SEQ ID NO:11, or a fragment thereof, the polypeptide or fragment specifically binds with an antibody from an autoimmune disease patient; and    the autoimmune disease is selected from the group consisting of rheumatoid arthritis, human systemic lupus erythematosus, Sjögren's syndrome, Beh et's disease, primary biliary cirrhosis, microscopic polyangitis/polyarteritis nodosa, ulcerative colitis, Crohn's disease, and autoimmune hepatitis.    
     
     
         12 . A kit for diagnosing an autoimmune disease, the kit comprising: 
 a first antigen selected from the group consisting of a polypeptide having an amino acid indicated by SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, or a fragment thereof, the polypeptide or fragment specifically binds with an antibody from an autoimmune disease patient;    a second antigen selected from the group consisting of a polypeptide having an amino acid sequence indicated by SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO: 1, or a fragment thereof, the polypeptide or fragment specifically binds with an antibody from an autoimmune disease patient;    a first component for detecting a first antigen-antibody complex; and    a second component for detecting a second antigen-antibody complex;    wherein the autoimmune disease is selected from the group consisting of rheumatoid arthritis, human systemic lupus erythematosus, Sjögren's syndrome, Beh et's disease, primary biliary cirrhosis, microscopic polyangitis/polyarteritis nodosa, ulcerative colitis, Crohn's disease, and autoimmune hepatitis.