Plant amino acyl-tRNA synthetase

Abstract

This invention relates to an isolated nucleic acid fragment encoding an aminoacyl-tRNA synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aminoacyl-tRNA synthetase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aminoacyl-tRNA synthetase in a transformed host cell.

1 - 30  (canceled)  
     
     
         31 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a polypeptide having the activity of cysteinyl-tRNA synthetase, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:14 have at least 80% identity based on the Clustal alignment method, or    (b) the complement of the nucleotide sequence.    
     
     
         32 . The polynucleotide of  claim 31 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:14 have at least 85% identity based on the Clustal alignment method.  
     
     
         33 . The polynucleotide of  claim 31 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:14 have at least 90% identity based on the Clustal alignment method.  
     
     
         34 . The polynucleotide of  claim 31 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:14 have at least 95% identity based on the Clustal alignment method.  
     
     
         35 . The polynucleotide of  claim 31 , wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:13.  
     
     
         36 . The polynucleotide of  claim 31 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:14.  
     
     
         37 . A chimeric gene comprising the polynucleotide of  claim 31  operably linked to a regulatory sequence.  
     
     
         38 . An isolated polynucleotide containing 30 nucleotides, wherein the nucleotide sequence containing 30 nucleotides is comprised by the polynucleotide of  claim 31 .  
     
     
         39 . A method for transforming a cell comprising transforming a cell with the polynucleotide of  claim 31 .  
     
     
         40 . A cell comprising the chimeric gene of  claim 37 .  
     
     
         41 . A method for producing a plant comprising transforming a plant cell with the polynucleotide of  claim 31  and regenerating a plant from the transformed plant cell.  
     
     
         42 . A plant comprising the chimeric gene of  claim 37 .  
     
     
         43 . A seed comprising the chimeric gene of  claim 37.