Method and apparatus for blood infrared spectroscopy diagnosing of inner organs pathology

Abstract

A rapid diagnosis method for organ specific disease state and an apparatus for carrying out the inventive method are based on obtaining an infrared absorbance spectra of a patient's blood at a frequency range from about 400 cm −1−1 to about 4000 cm. −1 . Comparing it with the predetermined normal infrared absorbance spectra of known healthy subjects for the presence or absence of predetermined features such as increase or decrease of infrared absorbance, peaks at particular frequencies allows for accurate diagnosis of a disease of an organ, including most major organs such as a heart, lungs, stomach, liver, kidneys, brain, etc.

1 . A method for rapid diagnosis of an organ specific disease state of a subject, using an infrared spectroscopy of a blood sample of said subject, said method comprising the steps of: irradiating said blood sample with an infrared light, obtaining an infrared absorbance spectra of said blood sample in a frequency ranging from about 400 cm. −1  to about 4000 cm. −1 , comparing said infrared absorbance spectra with a reference absorbance spectra correspondingly, identifying deviations of said infrared absorbance spectra from said reference infrared absorbance spectra in predetermined organ specific regions of said frequency range, and providing a diagnosis for an organ specific diseased state based on the presence of said deviations.  
     
     
         2 . The method as in  claim 1 , further including a step of determining a degree of said organ specific disease state based on intensity of said deviations.  
     
     
         3 . The method as in  claim 2 , further including examining a region of said infrared absorbance spectra in a frequency range from about 600 cm. −1  to about 700 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a state of hypoxia.  
     
     
         4 . The method as in  claim 2 , further including examining a region of said infrared absorbance spectra in a frequency range from about 700 cm. −1  to about 800 cm. −1  for a presence of deviations from said reference infrared absorbance spectra including a state of liver failure.  
     
     
         5 . The method as in  claim 2 , further including examining said region of said infrared absorbance spectra for a presence of three peaks at the frequencies of about 800 cm. −1  to about 900 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a disease state of an organ selected from a group of organs consisting of a colon, kidneys, and endocrine glands.  
     
     
         6 . The method as in  claim 2 , further including examining a region of said infrared absorbance spectra in a frequency range from about 900 cm. −1  to about 1000 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a diseased state of an organ selected from a group of organs consisting of a stomach, a pancreas, a duodenum, an jejunum, an ileum, an appendix, and also different tumors associated with immunodeficiency disorders.  
     
     
         7 . The method as in  claim 2 , further including examining a region of said infrared absorbance spectra in a frequency range from about 1000 cm. −1  to about 1130 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a diseased state of an organ selected from a group of organs, consisting of a liver and immune system.  
     
     
         8 . The method as in  claim 7 , further including examining a region of said infrared absorbance spectra in a frequency range from about 1100 cm. −1  to about 1200 cm. −1  for a presence of simultaneous deviations from said reference infrared absorbance spectra indicating a diseased state of upper respiratory tract and lungs.  
     
     
         9 . The method as in  claim 7 , further including examining a region of said infrared absorbance spectra in a frequency range from about 1160 cm. −1  to about 1350 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a diseased state of a heart.  
     
     
         10 . The method as in  claim 9 , further including examining a region of said infrared absorbance spectra in a frequency range from about 1200 cm. −1  to about 1350 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a diseased state of a heart conductive system.  
     
     
         11 . The method as in  claim 2 , further including examining a region of said infrared absorbance spectra in a frequency range from about 1400 cm −1  to about 1700 cm. −1  for a presence of deviations from said reference infrared absorbance spectra indicating a diseased state of brain and central nervous system.  
     
     
         12 . The method as in  claim 2 , further including examining a region of said infrared absorbance spectra in a frequency range from about 3000 cm. −1  to about 3100 cm. −1  for a presence of deviations from said reference infrared absorbance spectra, said presence indicating a state of chronic infection.  
     
     
         13 . The method as in  claim 5 , wherein the presence of deviations from said reference infrared absorbance spectra of 800 cm. −1  to about 900 cm. −1  further indicates a deceased state selected from the group consisting of acute nephritis, inactive form of chronic nephritis, correlated with nephrolithiasis and/or urinary sand, a disease of colon, acute colitis, chronic colitis, endocrine glands, diencephalic syndrome and obesity, severe endocrine disorder correlated with a disease state of pituitary dwarfism, congenital pigmented nevi, and thyroid gland, and disorders correlated with a disease state of hyperthyroidism and a combination thereof.  
     
     
         14 . The method as in  claim 6 , wherein the presence of deviations from said reference infrared absorbance spectra of 900 cm. −1  to about 1000 cm. −1  further indicates a deceased state selected from the group consisting of stomach disease, acute gastritis, chronic hypertrophic gastritis, chronic atrophic gastritis, chronic hypoacidic gastritis pancreatitis, duodenum disease, acute duodenitis, chronic duodenitis, jejunum, ileum, enteritis with terminal ileitis, acute catarrhous appendicitis, acute phlegmonous appendicitis, immunodeficiency disorders, malignant tumor disease known as retroperitoneal lymphoma, benign tumor disease known as congenital pigmented nevi, insufficiency of ileocecal valve, celiac sprue, acute brucellosis with primarily gastrointestinal complications (acute colitis and acute enteritis), lambliasis, and enterobiasis and a combination thereof.  
     
     
         15 . The method as in  claim 10 , wherein the presence of deviations from said reference infrared absorbance spectra of 1000 cm. −1  to about 1400 cm. −1  further indicates a diseased state consisting of the group selected from very severe immunodeficiency, correlated with malignant tumor, such as retroperitoneal lymphoma, severe immunodeficiency, correlated with benign tumor, such as congenital pigmented nevi, moderate immunodeficiency and allergy, correlated with Wiskott-Aldrich syndrome, allergy, correlated with bronchial asthma, acute cholecystitis, acute viral hepatitis, respiratory tract and lungs, acute pneumonia, acute viral respiratory infection, resolving neonatal pneumonia, myocardium, acute myocarditis, cardiomyopathy, myocardial infarction, heart insufficiency or heart failure, functional murmur, mitral valve prolapse, congenital atrial and ventricular septal defect, rheumatic mitral regurgitation, aortal stenosis, myocardial ischemia, and severe cardiac arrhythmia and a combination thereof.  
     
     
         16 . The method as in  claim 11 , wherein the presence of deviations from said reference infrared absorbance spectra of 1400 cm. −1  to about 1700 cm. −1  further indicates a diseased state consisting of the group selected from less severe functional damages of brain and central nervous system, such as neurosis, minor degree of intracranial hypertension, early stage of convulsion disorders, severe (organic, anatomic or inflammatory) damages of brain and central nervous system, such as head trauma, severe hypoxic ischemic perinatal encephalopathy with intracranial intraventricular hemorrhage, meningitis, cerebrosclerosis, neurosis, epilepsy, convulsion disorders, severe intracranial hypertension, minor degree of intracranial hypertension, severe hypoxic ischemic perinatal enceplopathy with intracranial intraventricular hemorrhage, hypoxic ischemic perinatal encephalopathy of a moderate degree, hypoxic ischemic perinatal encephalopathy of a mild degree, brain injury of a minor degree, postnatal head injury of a moderate degree, severe acute bacterial meningitis, cerebrosclerosis, rheumatic chorea, severe brain damage in a developmentally disabled blind infant with physical and mental retardation in one year after a postnatal head injury and a combination thereof.  
     
     
         17 . The method as in  claim 12 , wherein the presence of deviations from said reference infrared absorbance spectra of 3000-3100 cm. −1  further indicates a diseased state of pulmonary tuberculosis  
     
     
         18 . The method of  claim 4 , wherein the presence of deviations from said reference infrared absorbance spectra of about 600 cm. −1  to about 700 cm. −1  further indicates a diseased state consisting of the group selected from hypoxia, asphyxia of neonates, forceful strangling in adults, heart failure hypoxia.  
     
     
         19 . The method of  claim 1 , further comprising the step of preparing the blood sample by mixing dried blood with a filler in proportion of about 1.9-2.5 to about 190-300, preferably in proportion of about 2 mg. of dried blood to about 200 mg. of the filler.  
     
     
         20 . An apparatus for rapid diagnosis of an organ specific decease state of a subject, comprising: 
 a source of light generating a light beam in an infrared frequency range and propagating along an optical path to irradiate a blood sample placed along the optical path so as to obtain an infrared absorbance spectra of the blood sample; and    a processor provided with a software operative to identify deviations of the obtained infrared absorbance spectra from a reference spectra, wherein each of the identified deviations is indicative of the organ specific decease state of the subject.    
     
     
         21 . The apparatus of  claim 20 , wherein the obtained absorbance spectra ranges between about 400 cm −1  and about 4000 cm −1 .  
     
     
         22 . The apparatus of  claim 21 , wherein the obtained absorbance spectra ranges between about 400 cm −1  and about 2000 cm 1 .  
     
     
         23 . The apparatus of  claim 21 , wherein the obtained absorbance spectra ranges between about 3000 cm −1  and about 3100 cm −1 .  
     
     
         24 . The apparatus of  claim 20 , wherein the software operating on the processor is configured to detect an intensity of each of the identified deviations to determine a degree of the organ specific deceased state.  
     
     
         25 . The apparatus of  claim 20 , further comprising an electronic library configured to indicate a deceased state of a plurality of organs of the subject upon identifying each of the deviations.  
     
     
         26 . The apparatus of  claim 25 , wherein the plurality of organs is selected from the group consisting of heart, liver, colon, kidneys, endocrine glands, thyroid glands, stomach, pancreas, duodenum, jejunum, ileum, appendix, brain, central nervous system, of upper respiratory tract and lungs and a combination thereof.  
     
     
         27 . The apparatus of  claim 25 , wherein the deceased state is selected from the group consisting of heart failure hypoxia, liver failure, acute nephritis, active form of chronic nephritis, inactive form of chronic nephritis, correlated with nephrolithiasis, urinary sand, acute colitis, chronic colitis, mild endocrine disorders correlated with a decease state of diencephalic syndrome and obesity, severe endocrine disorder correlated with a decease state of pituitary dwarfism, severe endocrine disorder correlated with a decease state of congenital pigmented nevi, thyroid gland disorders correlated with a disease state of hyperthyroidism, acute gastritis, chronic hypertrophic gastritis, chronic hypertrophic gastritis, chronic hypoacidic gastritis, duodenum decease, acute duodenitis, chronic duodenitis, enteretis with terminal ileitis, acute catarrhous appendicitis, acute phlegmonous appendicitis, retroperitoneal lymphoma, congenital pigmented nevi, insufficiency of ileocecal valve, celiac sprue, lambliasis, enterobiasis, dolichocolon (or extreme elongated colon), immunodeficiency, correlated with malignant tumor, such as retroperitoneal lymphoma, severe immunodeficiency, correlated with benign tumor, such as congenital pigmented nevi, immunodeficiency and allergy, correlated with Wiskott-Aldrich syndrome, allergy, correlated with bronchial asthma, acute cholecystitis, acute viral hepatitis, pneumonia, viral respiratory infection, resolving neonatal pneumonia, myocardium cardiomyopathy, functional murmur, myocardial infarction, mitral valve prolapse, congenital atrial and ventricular septal defect, rheumatic mitral regurgitation, aortal stenosis, myocardial ischemia, neurosis, minor degree of intracranial hypertension, early stage of convulsion disorders, severe cardiac arrhythmia, head trauma, severe hypoxic ischemic perinatal encephalopathy with intracranial intraventricular hemorrhage, meningitis, cerebrosclerosis, neurosis, epilepsy, intracranial hypertension, neurosis, severe hypoxic ischemic perinatal enceplopathy with intracranial intraventicular hemorrhage, pulmonary tuberculosis and a combination thereof.  
     
     
         28 . The apparatus of  claim 20 , further comprising a heater operative to heat the blood sample so as to form a thin dry film, a mixer operative to mix the dried thin film with a filler and a press operative to compact the thin film mixed with the filler so as to form a solid state tablet.  
     
     
         29 . The apparatus of  claim 20 , wherein the processor is provided with a numerical display indicating the identified deviation and a spectral display.  
     
     
         30 . The apparatus of  claim 28 , wherein the thin dry film of blood is mixed with the filler in proportion of about 1.8 to about 210, preferably about 2 mg. of the thin dry film of blood being mixed with about 200 mg of the filler.