Reaction and separation methods

Abstract

A method of separating compounds that includes the steps of: tagging at least a first organic compound with a first tagging moiety to result in a first tagged compound; tagging at least a second organic compound with a second tagging moiety different from the first tagging moiety to result in a second tagged compound; and separating the first tagged compound from a mixture including the second tagged compound using a separation technique based upon differences between the first tagging moiety and the second tagging moiety. Preferably, the separation technique is based upon difference in the fluorous nature of the first tagged compound and the second tagged compound, differences in total charge between the first tagged compound and the second tagged compound, differences in size between the first tagged compound and the second tagged compound, and/or differences in polarity between the first tagged compound and the second tagged compound. The present invention also provides a method for carrying out a chemical reaction including the steps of: tagging a plurality of compounds with different tagging moieties to create tagged compounds, conducting at least one chemical reaction on a mixture of the tagged compounds to produce a mixture of tagged products, and separating the mixture of tagged products by a separation technique based upon differences in the tagging moieties.

1 . A method of separating compounds, the method comprising the steps of: 
 a. tagging at least a first organic compound with a first tagging moiety to result in a first tagged compound;    b. tagging at least a second organic compound with a second tagging moiety different from the first tagging moiety to result in a second tagged compound; and    c. separating the first tagged compound from a mixture including at least the second tagged compound using a separation technique based upon differences between the first tagging moiety and the second tagging moiety, the separation technique being based upon differences in total charge between the first tagged compound and the second tagged compound, differences in size between the first tagged compound and the second tagged compound, or differences in polarity between the first tagged compound and the second tagged compound.    
     
     
         2 . (Canceled)  
     
     
         3 . (Canceled).  
     
     
         4 . The method of  claim 1  wherein the first tagging moiety and the second tagging moiety differ in total charge.  
     
     
         5 . The method of  claim 4  wherein the first tagged compound and the second tagged compound are separated by electrophoresis.  
     
     
         6 . The method of  claim 1  wherein the first tagging moiety and the second tagging moiety are oligomers, polymers, or dendrimers that differ in size.  
     
     
         7 . The method of  claim 6  wherein the first tagged compound and the second tagged compound are separated by size exclusion chromatography.  
     
     
         8 . The method of  claim 1  wherein the first tagging moiety and the second tagging moiety differ in polarity.  
     
     
         9 . The method of  claim 8  wherein the first tagged compound and the second tagged compound are separated by standard or reverse phase chromatography.  
     
     
         10 . The method of  claim 1  wherein the first tagging moiety and the second tagging moiety are selected so that the order in which the first tagged compound and the second tagged compound separate is predetermined.  
     
     
         11 .- 16 . (Canceled)  
     
     
         17 . A method for carrying out a chemical reaction, comprising the steps of: tagging a plurality of compounds with different tagging moieties to create tagged compounds, conducting at least one chemical reaction on the tagged compounds to produce a mixture of tagged products, and separating at least one of the tagged products from the mixture of tagged products by a method based upon differences in the tagging moieties.  
     
     
         18 . The method of  claim 17  further including the step of removing the tagging moieties from the tagged products.  
     
     
         19 . The method of  claim 17  wherein the tagging moieties are fluorous moieties that differ in fluorous nature.  
     
     
         20 . The method of  claim 19  wherein the fluorous tagging moieties differ in fluorine content or structure.  
     
     
         21 . The method of  claim 19  wherein the tagged products are separated by using fluorous reverse phase chromatography.  
     
     
         22 . The method of  claim 17  wherein the tagging moieties differ in total charge.  
     
     
         23 . The method of  claim 22  wherein the tagged products are separated by electrophoresis.  
     
     
         24 . The method of  claim 17  wherein tagging moieties are oligomers, polymers, or dendrimers that differ in size.  
     
     
         25 . The method of  claim 24  wherein the tagged products are separated by size exclusion chromatography.  
     
     
         26 . The method of  claim 17  wherein the tagging moieties differ in polarity.  
     
     
         27 . The method of  claim 26  wherein the tagged products are separated by standard or reverse phase chromatography.  
     
     
         28 . The method of  claim 17  wherein all of the tagged compounds are reacted with the same starting compounds.  
     
     
         29 . The method of  claim 17  wherein a first plurality of compounds is tagged with a first tagging moiety and mixed with other tagged compounds that are tagged with tagging moieties different from the first tagging moiety.  
     
     
         30 . The method of  claim 29  wherein the separation of the tagged products provides mixtures of product compounds bearing the same initial tag.  
     
     
         31 . The method of  claim 17  wherein the tagged compounds are reacted with a mixture of different starting compounds.  
     
     
         32 . The method of  claim 17  wherein the tagged compounds are selectively tagged with different tagging moieties such that the tagged products separate into fractions of known identity as determined by the tagging moieties.  
     
     
         33 . A method for carrying out a chemical reaction, comprising the steps of: tagging a plurality of compounds with different fluorous tagging moieties to create tagged compounds, conducting at least one chemical reaction on the tagged compounds to produce a mixture of tagged products, and separating at least one of the tagged products from the mixture of tagged products by a method based upon differences in the fluorous nature of the tagging moieties.  
     
     
         34 . The method of  claim 33  further including the step of removing the fluorous tagging moieties from the tagged products.  
     
     
         35 . The method of  claim 33  wherein the fluorous tagging moieties differ in fluorine content or structure.  
     
     
         36 . The method of  claim 35  wherein the tagged products are separated using fluorous reverse phase chromatography.  
     
     
         37 . The method of  claim 33  wherein a first plurality of compounds is tagged with a first tagging moiety and mixed with other tagged compounds that are tagged with tagging moieties different from the first tagging moiety.  
     
     
         38 . The method of  claim 33  wherein the separation of the tagged products provides mixtures of product compounds bearing the same initial tag.  
     
     
         39 . The method of  claim 33  wherein the tagged compounds are reacted with a mixture of different starting compounds.  
     
     
         40 . The method of  claim 33  wherein all of the tagged compounds are reacted with the same starting compounds.  
     
     
         41 . The method of  claim 33  wherein a first plurality of compounds is tagged with a first fluorous tagging moiety and mixed with other tagged compounds that are tagged with fluorous tagging moieties different from the first tagging moiety.  
     
     
         42 . The method of  claim 41  wherein the separation of the tagged products provides mixtures of product compounds bearing the same initial tag.  
     
     
         43 . The method of  claim 33  wherein the tagged compounds are reacted with a mixture of different starting compounds.  
     
     
         44 . The method of  claim 33  wherein the tagged compounds are selectively tagged with different tagging moieties such that the tagged products separate into fractions of known identity as determined by the tagging moieties.  
     
     
         45 . A method of separating compounds, the method comprising the steps of: 
 a. tagging a first organic compound with a first tagging moiety to result in a first tagged compound;    b. tagging at least a second organic compound with a second tagging moiety different from the first tagging moiety to result in a second tagged compound; and    c. separating the first tagged compound into a predetermined fraction from a mixture including at least the second tagged compound using a separation technique based upon differences between the first tagging moiety and the second tagging moiety, wherein the predetermined fraction and the identity of the first tagged compound in the predetermined fraction are determined by the first tagging moiety.