US2004191924A1PendingUtilityA1

Reformatted through-hole arrays

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Assignee: MASSACHUSETTS INST TECHNOLOGYPriority: Jan 12, 1998Filed: Apr 8, 2004Published: Sep 30, 2004
Est. expiryJan 12, 2018(expired)· nominal 20-yr term from priority
Inventors:Ian W. HunterColin BrenanTanya S. Kanigan
B01L 2300/0819B01J 2219/00283B01J 2219/00659B01J 2219/00686B01L 3/50857G01N 2035/1037B01J 2219/00373B01J 2219/00369B01J 2219/00387B01J 2219/00648Y10T436/25B01L 2200/0642B01L 3/5025B01L 2300/0829B01L 3/0293B01L 2300/0845B01J 2219/00536B01J 2219/00351B01J 19/0046B01J 2219/00587B01J 2219/00389G01N 30/466B01L 3/5085B01J 2219/00479B01J 2219/00319G01N 30/82B01J 2219/005B01F 33/30351B01F 33/3021C40B 70/00G01N 35/00069C40B 60/14B01J 2219/00585Y10T436/25625B01J 2219/0036B01J 2219/00511B01L 3/0262B01L 3/50255B01L 9/523B01J 2219/00549B01J 2219/00533B01L 2400/022G01N 2035/00237Y10T436/2575Y10T436/11B01J 2219/00317G01N 30/6095Y10T83/0481B01J 2219/0043B01J 2219/00423B01J 2219/00495B01J 2219/00596
54
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Claims

Abstract

Methods for loading a plurality of liquid samples into a through-hole array. Each transfer member of an array of transfer members is positioned for drawing a liquid sample from a distinct well of a microtiter plate. Liquid samples are drawn from wells of the microtiter plate and the array of transfer members is registered with a subset of through-holes of a through-hole array. The liquid samples are then dispensed from the transfer members into through-holes of the through-hole array.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A method for loading a plurality of liquid samples into a through-hole array, the liquid samples residing in wells of a microtiter plate characterized by a well-to-well spacing, the through-hole array characterized by a hole spacing that is an integral fraction of the well-to-well spacing of the microtiter plate, the method comprising: 
 a. positioning an array of transfer members, each transfer member disposed for drawing liquid from a distinct well of the microtiter plate;    b. drawing liquid samples from the wells of the microtiter plate to each of the transfer members;    c. registering the array of transfer members with a subset of through-holes of the through-hole array; and    d. dispensing the liquid samples from the transfer members into through-holes of the through-hole array.    
     
     
         2 . A method for loading a plurality of liquid samples in accordance with  claim 1 , further including stacking a plurality of through-hole arrays in registration such that liquid samples are deposited from each transfer member into a plurality of through-holes.  
     
     
         3 . A method in accordance with  claim 1 , wherein the step of dispensing the liquid samples from the transfer members includes expelling liquid from a capillary.  
     
     
         4 . A method in accordance with  claim 1 , wherein the step of dispensing the liquid samples from the transfer members includes expelling liquid from tubing of a pipette.  
     
     
         5 . A method in accordance with  claim 1 , wherein the step of dispensing the liquid samples from the transfer members includes expelling liquid by means of a syringe.  
     
     
         6 . A method in accordance with  claim 1 , wherein the step of drawing liquid samples from the wells of the microtiter plate includes drawing liquid into a capillary.  
     
     
         7 . A method in accordance with  claim 1 , wherein the step of drawing liquid samples from the wells of the microtiter plate includes drawing liquid into tubing of a pipette.  
     
     
         8 . A method in accordance with  claim 1 , wherein the step of drawing liquid samples from the wells of the microtiter plate includes drawing liquid by means of a syringe.

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