DNA sequences coding for a mammalian glucuronyl C5-epimerase and a process for its production

Abstract

An isolated or recombinant DNA sequence coding for a mammalian, including human, glucuronyl C5-epimarase or a functional derivative thereof capable of converting D-glucuronic acid (GlcA) to L-iduronic acid (IdoA); a recombinant expression vector comprising such DNA sequence; a host cell transformed with such recombinant expression vector; a process for the manufacture of a glucuronyl C5-epimerase or functional derivative thereof capable of converting GlcA to IdoA, comprising cultivation of a cell-line transformed with such recombinant expression vector; and a glucuronyl C5-epimerase or functional derivative thereof prepared by such process.

1 . An isolated or recombinant DNA sequence coding for a mammalian, including human, glucuronyl C5-epimerase or a functional derivative thereof capable of converting D-glucuronic acid (GlcA) to L-iduronic acid (IdoA).  
     
     
         2 . A DNA sequence according to  claim 1  constituted by a nucleotide sequence comprising nucleotide residues 1 to 1404, inclusive, as depicted in the sequence listing.  
     
     
         3 . A DNA sequence according to  claim 2  constituted by a nucleotide residue comprising nucleotide residues 73 to 1404, inclusive, as depicted in the sequence listing.  
     
     
         4 . A DNA sequence according to  claim 2  constituted by a nucleotide residue comprising nucleotide residues 1 to 1404, inclusive, as depicted in the sequence listing.  
     
     
         5 . A recombinant expression vector containing a transcription unit comprising a DNA sequence according to any one of the preceding claims, a transcriptional promoter, and a polyadenylation sequence.  
     
     
         6 . A host cell transformed with the recombinant expression vector of  claim 5 .  
     
     
         7 . A process for the manufacture of a glucuronyl C5-epimerase or a functional derivative thereof capable of converting D-glucuronic acid (GlcA) to L-iduronic acid (IdoA), comprising cultivation of a cell line transformed with a recombinant expression vector according to  claim 5  in a nutrient medium allowing expression and secretion of said epimerase or functional derivative thereof.  
     
     
         8 . A glucuronyl C5-epimerase or a functional derivative thereof whenever prepared by the process of  claim 7.