Apparatus for microinjection of sample into amphibian oocytes

Abstract

An apparatus for microinjection of samples into amphibian oocytes, comprising a tray for holding a plurality of the amphibian oocytes, an injection needle for injecting a sample into the said amphibian oocytes, a driving means for moving a relative position between the said tray and the said injection needle and a controlling means for controlling the said movement by imputing a depth of the said injection needle for the said tray or the said amphibian oocytes in the injection of the sample, and injecting the sample into the said amphibian oocytes at the said depth. According to the present invention, the sample can be injected into the amphibian oocyte with constant depth precisely and quality of oocyte or a positional site of needle injection can be recorded as the information.

What is claimed is:  
     
         1 . A plurality of amphibian oocytes into which samples are injected under a substantially equal condition in the injection depth.  
     
     
         2 . The plurality of amphibian oocytes according to  claim 1 , wherein the said sample is further injected under a substantially equal condition in the injection area.  
     
     
         3 . The plurality of amphibian oocytes according to  claim 1 , wherein an amount of injection of the said sample is also substantially constant.  
     
     
         4 . A vessel containing amphibian oocytes into which the sample has been injected under a substantially same condition in an injection depth, and having the information on date and time of the injection and the information on expiration date for use attached thereto.  
     
     
         5 . The vessel according to  claim 4 , wherein the amphibian oocytes, into which the sample has also been injected under a substantially equal condition in at least any one of the injection area or the amount of the injection, are contained.  
     
     
         6 . A method for preparation of amphibian oocytes injected with a sample comprising, 
 using an apparatus having a tray for holding a plurality of the amphibian oocytes and an injection needle for injecting a sample into the said amphibian oocytes,    moving a relative position of the said injection needle to the said tray,    injecting the sample into each of the said amphibian oocytes using the said injection needle,    obtaining visual information of each of the said amphibian oocytes in the said injection, and    collecting a plurality of the oocytes in which the sample is injected into the animal hemisphere of the oocyte in the said a plurality of amphibian oocytes, based on the said visual information.    
     
     
         7 . A method for preparation of amphibian oocytes injected with a sample comprising, 
 using an apparatus having a tray for holding a plurality of the amphibian oocytes and an injection needle for injecting a sample into the said amphibian oocytes,    moving a relative position of the said injection needle to the said tray,    injecting the sample into each of the said amphibian oocytes using the said injection needle,    obtaining visual information of each of the said amphibian oocytes in the said injection, and    collecting a plurality of the oocytes in which the sample is injected into the vegetal hemisphere of the oocyte in the said plurality of amphibian oocytes, based on the the said visual information.    
     
     
         8 . The method for preparation of amphibian oocytes according to  claim 6  comprising, 
 setting a depth of the said injection needle for the sid amphibian oocytes or the said tray to the first depth prior to the said injection, and  
 injecting the sample into a plurality of the said amphibian oocytes at the said first depth.  
 
     
     
         9 . A method to use a plurality of the amphibian oocytes into which a gene or a protein is injected at an approximately constant position and a depth, as a sensor for screening.