US2004157320A1PendingUtilityA1

Low fluorescence nylon/glass composites for micro-analytical diagnostic applications

51
Priority: Jul 5, 2000Filed: Feb 5, 2004Published: Aug 12, 2004
Est. expiryJul 5, 2020(expired)· nominal 20-yr term from priority
Inventors:Rita AndreoliMurtaza Y. AminMark MeyeringRichard ChestersonEugene A. Ostreicher
C07H 21/00B01J 2219/00274B01J 2219/00576B01J 2219/00585B01J 2219/00596B01J 2219/00641B01J 2219/00644B01J 2219/00657B01J 2219/00659B01J 2219/00662B01J 2219/00722B01J 2219/00725C07B 2200/11C40B 40/00G01N 33/54353G01N 33/54393G01N 33/545G01N 33/551
51
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Claims

Abstract

An improved combination non-luminescent microporous membrane and solid support for use in micro-analytical diagnostic applications is disclosed. Specifically, composite microarray non-luminescent slides having a microporous membrane effectively attached by covalent bonding through a surface treatment to a substrate that prepares the substrate to sufficiently, covalently bond to the non-luminescent microporous membrane formed by a phase inversion process such that the combination produced thereby is useful in microarray applications and wherein the porous non-luminescent nylon composite microarray slides are covalently bonded to a solid base member, such as, for example, a glass or Mylar microscope slide, such that the combination produced thereby is useful in microarray applications. Apparatus and methods for fabricating the non-luminescent composite microarray slides are also disclosed.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A method of fabricating non-luminescent composite microarray slides useful for carrying a microarray of biological polymers comprising the acts of: 
 providing a non-porous substrate;    providing a non-luminescent microporous membrane formed by a phase inversion process, the process comprising the acts of: 
 formulating a dope comprising a solvent, one or more non-solvents, opaque solids, and polyamide(s);  
 mixing the dope to cause dissolution of the polyamide and opaque solids therein;  
 producing an opaque solids-filled phase inversion dope;  
 casting a portion of the opaque solids-filled phase inversion dope; and  
 quenching the cast portion of the opaque solids-filled phase inversion dope to form a non-luminescent, microporous membrane;  
   providing a surface treatment;    applying the surface treatment to the non-porous substrate; and    intermingling the non-porous substrate having the surface treatment with the non-luminescent, microporous membrane such that the non-porous substrate is sufficiently covalently bonded to the non-luminescent microporous membrane wherein the combination produced thereby is useful in microarray applications.    
     
     
         2 . The method of  claim 1  wherein the surface treatment is selected from the group comprising: 
 3-aminopropyl triethoxysilane, N-(2-aminoethyl)-3-aminopropyl trimethoxysilane, 3-glycidoxypropyltrimethoxysilane, (10-carbomethoxydecyl) dimethylchlorosilane or 2-(3,4-epoxycyclohexyl)-ethyltrimethoxysilane.  
 
     
     
         3 . The method of  claim 1  wherein, the surface treatment comprises a 3-aminopropyl triethoxysilane followed by treatment with a polyamido-polyamine epichlorohydrin resin.  
     
     
         4 . The method of  claim 1  wherein, the non-porous substrate is selected from the group comprising: 
 glass, Mylar, ceramic, acrylic, polypropylene, polycarbonate, polysulfone, polyamide and polyaramid.  
 
     
     
         5 . The method of  claim 1  wherein, the non-porous substrate is glass.  
     
     
         6 . The method of  claim 1  wherein, the non-porous substrate is a polyester.  
     
     
         7 . The method of  claim 1  wherein, the non-porous substrate is Mylar.  
     
     
         8 . The method of  claim 7  wherein, the surface of the Mylar is oxidized with sulfuric acid or corona discharge to enable it to bond to a polyamido-polyamine epichlorohydrin polymer.  
     
     
         9 . The method of  claim 1  wherein the opaque solids are carbon particles.  
     
     
         10 . The method of  claim 1  wherein the carbon particles are less than 5 microns in size.  
     
     
         11 . The method of  claim 1  wherein the carbon particles are substantially uniformly distributed throughout the non-luminescent microporous membrane.  
     
     
         12 . The method of  claim 1  wherein the carbon particles are partially incorporated into the non-luminescent microporous membrane.  
     
     
         13 . The method of  claim 1  wherein the carbon particles are substantially wholly incorporated into the non-luminescent microporous membrane.  
     
     
         14 . The method of  claim 1  wherein the non-luminescent microporous membrane is charge-modified.  
     
     
         15 . A composite microarray slide, useful for carrying a microarray of biological polymers comprising: 
 a substantially non-reflective microporous membrane which provides little fluorescence from about three hundred (300) nm to about seven hundred (700) nm formed by a phase inversion process, the non-reflective microporous membrane comprising: 
 a phase inversion support; and  
 a plurality of opaque solids that are substantially chemically non-reactive with the phase inversion support and intimately bound to, and/or partially/completely contained within, said phase-inversion;  
   a non-porous substrate; and    a surface treatment, operatively positioned between the substantially non-reflective microporous membrane and the non-porous substrate, for sufficiently covalently bonding the non-porous substrate to the microporous membrane wherein the combination composite microarray slides produced thereby are useful in microarray applications.    
     
     
         16 . The composite microarray slide of  claim 15  wherein, the surface treatment is selected from the group comprising: 
 3-aminopropyl triethoxysilane, N-(2-aminoethyl)-3-aminopropyltrimethoxysilane, 3-glycidoxypropyltrimethoxysilane, (10-carbomethoxydecyl) dimethylchlorosilane or 2-(3,4-epoxycyclohexyl)-ethyltrimethoxysilane.  
 
     
     
         17 . The composite microarray slide of  claim 15  wherein, the non-porous substrate is selected from the group comprising: 
 glass, Mylar, ceramic, acrylic, polypropylene, polycarbonate, polysulfone, polyamide and polyaramid.  
 
     
     
         18 . The composite microarray slide of  claim 15  wherein, the surface treatment comprises a 3-aminopropyl triethoxysilane followed by treatment with a polyamido-polyamine epichlorohydrin resin.  
     
     
         19 . The composite microarray slide of  claim 15  wherein, the non-porous substrate is glass.  
     
     
         20 . The composite microarray slide of  claim 15  wherein, the non-porous substrate is a polyester.  
     
     
         21 . The composite microarray slide of  claim 15  wherein the, the non-porous substrate is Mylar.  
     
     
         22 . The composite microarray slide of  claim 15  wherein the membrane is selected from the group consisting of: 
 Nylon 66, Nylon 46, Nylon 6, polysulfone, polyethersulfone, polyvinylidenediflouride (PVDF).  
 
     
     
         23 . The composite microarray slide of  claim 15  wherein the phase-inversion support comprises polyamides.  
     
     
         24 . The composite microarray slide of  claim 15  wherein the opaque solids are pigments.  
     
     
         25 . The composite microarray slide of  claim 15  wherein the opaque solids are carbon particles.  
     
     
         26 . The composite microarray slide of  claim 15  wherein the phase inversion support has been charge-modified.  
     
     
         27 . The composite microarray slide of  claim 15  wherein carbon particles are less than five microns in size.  
     
     
         28 . The composite microarray slide of  claim 15  wherein carbon particles are substantially uniformly distributed throughout the phase-inversion support.  
     
     
         29 . The composite microarray slide of  claim 15  wherein the carbon particles are partially incorporated into the phase-inversion support.  
     
     
         30 . The composite microarray slide of  claim 15  wherein the carbon particles are substantially wholly incorporated into the phase-inversion support.  
     
     
         31 . The composite microarray slide of  claim 15  wherein the phase-inversion support has been charge-modified.  
     
     
         32 . Composite microarray slides, useful for carrying a microarray of biological polymers comprising: 
 an optically passive substrate comprising:    a phase-inversion support and opaque solids that are substantially non-reactive chemically with the phase-inversion support, in a weight ratio with the phase-inversion support such that the optically passive substrate absorbs light at substantially all wave lengths from about 300 nm to about 700 nm;    a non-porous substrate; and    a surface treatment, operatively positioned between the optically passive substrate and the non-porous substrate, for sufficiently covalently bonding the non-porous substrate to the optically passive substrate wherein the combination composite microarray slides produced thereby is useful in microarray applications.    
     
     
         33 . The composite microarray slide of  claim 32  wherein the optically passive substrate comprises polyamide.  
     
     
         34 . The composite microarray slide of  claim 32  wherein the optically passive substrate is in the form of a membrane.  
     
     
         35 . The composite microarray slide of  claim 32  wherein the opaque solids are carbon particles.  
     
     
         36 . The composite microarray slide of  claim 35  wherein the carbon particles are less than about 5 microns in size.  
     
     
         37 . The composite microarray slide of  claim 35  wherein the carbon particles are substantially uniformly distributed throughout the optically passive substrate.  
     
     
         38 . The composite microarray slide of  claim 35  wherein the carbon particles are partially incorporated into the optically passive substrate.  
     
     
         39 . The composite microarray slide of  claim 37  wherein the optically passive substrate absorbs light at substantially all wavelengths from about 300 to about 700 nm.  
     
     
         40 . The composite microarray slide of  claim 32  wherein the phase-inversion support has been charge-modified.  
     
     
         41 . The composite microarray slide of  claim 39  wherein the optically passive substrate has a reflectance of no more than 50% of incident light at any wavelength within about 300 to about 700 nm.  
     
     
         42 . The composite microarray slide of  claim 32  wherein the phase-inversion support is hydrophilic.  
     
     
         43 . The composite microarray slide of  claim 42  wherein the phase-inversion support is skinless.  
     
     
         44 . The composite microarray slide of  claim 43  wherein the phase-inversion support comprises nylon.  
     
     
         45 . The method of  claim 1  wherein the polyamide(s) is selected from the group consisting of: 
 Nylon 66, Nylon 46, Nylon 6, polysulfone, polyethersulfone, polyvinylidenediflouride (PVDF).

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