Diagnostics and therapeutics for diseases associated with an IL-1 inflammatory haplotype

Abstract

Methods and kits for determining whether a subject has or is predisposed to developing a disease which is associated with IL-1 polymorphisms and assays for identifying therapeutics for treating and/or preventing the development of these diseases are provided.

What is claimed is:  
     
         1 . A kit comprising a means for detecting one or more alleles selected from the group consisting of: allele 4 of the 222/223 marker of IL-1A, allele 4 of the gz5/gz6 marker of IL-1A, allele 1 of the −889 marker of IL-1A, allele 1 of the +3954 marker of IL-1B, allele 2 of the −511 marker of IL-1B, allele 3 of the gaat.p33330 marker, allele 3 of the Y31 marker, allele 2 of +2018 of IL-1RN, allele 1 of +4845 of IL-1A, allele 3 of the 222/223 marker of IL-1A, allele 3 of the gz5/gz6 marker of IL-1A, allele 2 of the −889 marker of IL-1A, allele 2 of the +3954 marker of IL-1B, allele 1 of the −511 marker of IL-1B, allele 4 of the gaat.p33330 marker, allele 6 of the Y31 marker, allele 1 of +2018 of IL-1RN, allele 2 of +4845 of IL-1A, and allele 1 of the VNTR marker of IL-1RN.  
     
     
         2 . A kit comprising a means for detecting at least two alleles selected from the group consisting of: allele 2 of 1731 IL-RN, allele 2 of 1812 IL-1RN, allele 2 of 1868 IL-1RN, allele 2 of 1887 IL-1RN, allele 2 of 8006 IL-1RN, allele 2 of 8061 IL-1RN, allele 2 of 9589 IL-1RN, allele 4 of the 222/223 marker of IL-1A, allele 4 of the gz5/gz6 marker of IL-1A, allele 1 of the −889 marker of IL-1A, allele 1 of the +3954 marker of IL-1B, allele 2 of the −511 marker of IL-1B, allele 3 of the gaat.p33330 marker, allele 3 of the Y31 marker, allele 2 of +2018 of IL-1RN, allele 1 of +4845 of IL-1A, allele 3 of the 222/223 marker of IL-1A, allele 3 of the gz5/gz6 marker of IL-1A, allele 2 of the −889 marker of IL-1A, allele 2 of the +3954 marker of IL-1B, allele 1 of the -511 marker of IL-1B, allele 4 of the gaat.p33330 marker, allele 6 of the Y31 marker, allele 1 of +2018 of IL-1RN, allele 2 of +4845 of IL-1A, allele 2 of the VNTR marker of IL-1RN, and allele 1 of the VNTR marker of IL-1RN.  
     
     
         3 . The kit of  claim 1  or  2 , wherein said detecting means is selected from the group consisting of: 
 a) allele specific oligonucleotide hybridization;  
 b) size analysis;  
 c) sequencing;  
 d) hybridization;  
 e) 5′ nuclease digestion;  
 f) single-stranded conformation polymorphism;  
 g) allele specific hybridization;  
 h) primer specific extension; and  
 j) oligonucleotide ligation assay.  
 
     
     
         4 . The kit of  claim 1 , further comprising a means for detecting allele 2 of the VNTR marker of IL-1RN.  
     
     
         5 . A kit comprising an isolated nucleic acid molecule which hybridizes to an allele selected from the group consisting of: allele 2 of 1731 IL-RN, allele 2 of 1812 IL-1RN, allele 2 of 1868 IL-1RN, allele 2 of 1887 IL-1RN, allele 2 of 8006 IL-1RN, allele 2 of 8061 IL-1RN, allele 2 of 9589 IL-1RN, allele 4 of the 222/223 marker of IL-1A, allele 4 of the gz5/gz6 marker of IL-1A, allele 1 of the −889 marker of IL-1A, allele 1 of the +3954 marker of IL-1B, allele 2 of the −511 marker of IL-1B, allele 3 of the gaat.p33330 marker, allele 3 of the Y31 marker, allele 2 of +2018 of IL-1RN, allele 1 of +4845 of IL-1A, allele 3 of the 222/223 marker of IL-1A, allele 3 of the gz5/gz6 marker of IL-1A, allele 2 of the −889 marker of IL-1A, allele 2 of the +3954 marker of IL-1B, allele 1 of the −511 marker of IL-1B, allele 4 of the gaat.p33330 marker, allele 6 of the Y31 marker, allele 1 of +2018 of IL-1RN, allele 2 of +4845 of IL-1A, allele 2 of the VNTR marker of IL-1RN, and allele 1 of the VNTR marker of IL-1RN.  
     
     
         6 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 1731 IL-RN.  
     
     
         7 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 1812 IL-1RN.  
     
     
         8 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 1868 IL-1RN.  
     
     
         9 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 1887 IL-1RN.  
     
     
         10 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 8006 IL-1RN.  
     
     
         11 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 8061 IL-1RN.  
     
     
         12 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of 9589 IL-1RN.  
     
     
         13 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 4 of the 222/223 marker of IL-1A or allele 3 of the 222/223 marker of IL-1A.  
     
     
         14 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 4 of the gz5/gz6 marker of IL-1A or allele 3 of the gz5/gz6 marker of IL-1A.  
     
     
         15 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 1 of the −889 marker of IL-1A or allele 2 of the −889 marker of IL-1A.  
     
     
         16 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 1 of the +3954 marker of IL-1B or allele 2 of the +3954 marker of IL-1B.  
     
     
         17 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of the −511 marker of IL-1B or allele 1 of the −511 marker of IL-1B.  
     
     
         18 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 3 of the gaat.p33330 marker or allele 4 of the gaat.p33330 marker.  
     
     
         19 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 3 of the Y31 marker or allele 6 of the Y31 marker.  
     
     
         20 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of +2018 of IL-1RN or allele 1 of +2018 of IL-1RN.  
     
     
         21 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 1 of +4845 of IL-1A or allele 2 of +4845 of IL-1A.  
     
     
         22 . The kit of  claim 5 , wherein said isolated nucleic acid molecule hybridizes to the nucleotide corresponding to allele 2 of the VNTR marker of IL-1RN or allele 1 of the VNTR marker of IL-1RN.