US2004152074A1PendingUtilityA1
Seroreactive regions on HPV 16 proteins E1 and E2
Est. expiryJul 18, 2011(expired)· nominal 20-yr term from priority
C12N 2710/20022A61P 35/00A61P 31/12A61K 2039/5256A61P 31/20C07K 14/005C12N 7/00C07K 16/084A61K 39/00
65
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Claims
Abstract
The invention relates to seroreactive regions on proteins E1 and E2 of human papillomavirus (HPV) 16. The application also relates to a vaccine which contains such peptides which contain the seroreactive regions. The invention likewise embraces compositions for diagnostic purposes which contain peptides with the seroreactive regions.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . Seroreactive regions on the E1 protein of human papillomavirus (HPV) 16, with the following amino-acid sequences:
I.
NGWFYVEAVVEXKTGDAISDDENENDSDTGEDLVDFIVNDNDYLT
II.
NENDSDTGEDLVDFIVND
III.
NADPAGTNGEEGTGCNGWFYVEAVVEKKTGDAISDPENENDSDTGE
DLVDFIVNDNDYLT
IV.
EDLVDFIVNDNDYLT
V.
EDLVDFIVNDNDYLTQAETETAHALFTAQEKQH
VI.
NENDSDTGEDLVDFIVNDNDYLTQAETETAHALFTAQEAXQHRDA
VQVLKRKYL
VII.
GSPLSDIS.
2 . Seroreactive regions on the E2 protein of human papillomavirus (HPV) 16, with the following amino-acid sequences:
I.
DKILTHYENDS
II.
DKILTHYENDSTDLRDHI
III.
DLRDHIDYWKH
IV.
AIYYKAREMGFKHINHQVVPTLA
V.
AIYYKAREMGFKHINHQVVPTLAVSKNKAL
VI.
YYKAREMGFKHINHQVVPTLAVSKN
VII.
INHQVVPTLAVSKNKALQAI
VIII.
INHQVVPTLAVSKNKAL
IX.
TLAVSKNKALQAIELQLTLETIYNSQYSNEKWTLQDV
X.
QLTLETIYNSQYSNEKWTLQDVSLE
XI.
TLETIYNSQYSNEK
XII.
TSVFSSNEVSSPEII
XIII.
VFSSNEVSSPEIIRQHLANHPAATHTKAVALGTEET
XIV.
EIIRQHLANHPAATHTKAVALGTEETQTTIQRPRSEP
3 . A peptide which contains one or more of the seroreactive regions as claimed in claim 1 .
4 . A peptide which contains one or more of the seroreactive regions as claimed in claim 2 .
5 . A vaccine which contains one or more of the peptides as claimed in claim 3 .
6 . A vaccine which contains one or more of the peptides as claimed in claim 4 .
7 . A composition for diagnostic purposes for identifying specific antibodies against HPV 16 E1 or E2 protein, which contains peptides as claimed in claim 3 .
8 . A composition for diagnostic purposes for identifying specific antibodies against HPV 16 E1 or E2 protein, which contains peptides as claimed in claim 4 .
9 . A monoclonal antibody which has affinity for the seroreactive regions of claim 1 .
10 . A monoclonal antibody which has affinity for the seroreactive regions of claim 2 .
11 . A composition for diagnostic purposes, which contains a monoclonal antibody as claimed in claim 9 .
12 . A composition for diagnostic purposes, which contains a monoclonal antibody as claimed in claim 10 .
13 . A composition for diagnostic purposes as claimed in claim 11 for identifying HPV 16 specific E1 or E2 proteins.
14 . A composition for diagnostic purposes as claimed in claim 12 for identifying HPV 16 specific E1 or E2 proteins.
15 . The use of peptides as claimed in claim 3 for producing vaccine or for compositions for diagnostic purposes.
16 . The use of peptides as claimed in claim 4 for producing vaccine or for compositions for diagnostic purposes.
17.
An antibody that binds to an amino acid sequence present in SEQ
ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID
NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID
NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:17, SEQ ID
NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, or SEQ ID NO:22.
18 . The antibody of claim 17 , wherein the antibody detects or identifies the E1 or E2 protein of human papilloma virus 16 (HPV 16).
19 . The antibody of claim 17 , which is a monoclonal antibody.
20 . The antibody of claim 17 , which is a polyclonal antibody.
21 . Polyclonal antisera comprising the antibody of claim 17 .
22 . A composition comprising the antibody of claim 17 .
23 . The composition of claim 22 , which is a diagnostic composition.
24 . The composition of claim 22 , wherein the composition is used for identifying, diagnosing, or detecting HPV 16 infection.
25 . A method of identifying, diagnosing, or detecting HPV 16 infection, said method comprising
contacting a sample from a human suspected of being infected with HPV 16 with an antibody that binds to an amino acid sequence present in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID Nb:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, or SEQ ID NO:22, and detecting binding of said antibody to said amino acid sequence, wherein binding indicates an HPV 16 infection.
26 . The method of claim 25 , wherein said detecting is by enzyme linked immunosorbent assay (ELISA).
27 . The method of claim 25 , wherein said method detects binding of said antibody with the E1a, E1b, or E2 protein of HPV 16.Join the waitlist — get patent alerts
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