US2004142427A1PendingUtilityA1

Polynucleotide encoding a polypeptide having heparanase activity and expression of same in genetically modified cells

65
Priority: Aug 31, 1998Filed: Feb 25, 2004Published: Jul 22, 2004
Est. expiryAug 31, 2018(expired)· nominal 20-yr term from priority
Inventors:Iris PeckerIsrael VlodavskyElena Feinstein
C12N 9/2402C12Y 302/01166A61K 38/00
65
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

A polynucleotide (hpa) encoding a polypeptide having heparanase activity, vectors including same, genetically modified cells expressing heparanase, a recombinant protein having heparanase activity and antisense oligonucleotides and constructs for modulating heparanase expression.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A polynucleotide fragment comprising a polynucleotide sequence encoding a polypeptide having heparanase catalytic activity, wherein said polypeptide shares at least 70% homology with SEQ ID NOs:10, 14 or 44, as determined using default parameters of a DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the University of Wisconsin.  
     
     
         2 . The polynucleotide fragment of  claim 1 , wherein said polynucleotide sequence includes nucleotides 63-1691 of SEQ ID NO:9.  
     
     
         3 . The polynucleotide fragment of  claim 1 , wherein said polynucleotide sequence includes nucleotides 63-721 of SEQ ID NO:9.  
     
     
         4 . The polynucleotide fragment of  claim 1 , wherein said polynucleotide is as set forth in SEQ ID NO:9.  
     
     
         5 . The polynucleotide fragment of  claim 1 , wherein said polynucleotide sequence includes a segment of SEQ ID NO:9, said segment encodes said polypeptide having said heparanase catalytic activity.  
     
     
         6 . The polynucleotide fragment of  claim 1 , wherein said polypeptide includes an amino acid sequence as set forth in SEQ ID NOs:10, 14 or 44.  
     
     
         7 . The polynucleotide fragment of  claim 1 , wherein said polypeptide includes a segment of SEQ ID NOs:10, 14 or 44 said segment harbors said heparanase catalytic activity.  
     
     
         8 . The polynucleotide fragment of  claim 1 , wherein said polynucleotide-sequence is selected from the group consisting of double stranded DNA, single stranded DNA and RNA.  
     
     
         9 . A polynucleotide sequence as set forth in SEQ ID NOs:9, 13, 42 or 43.  
     
     
         10 . A polynucleotide sequence at least 70% homologous to SEQ ID NOs:9, 13, 42 or 43, as determined using default parameters of a DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the University of Wisconsin, wherein said polynucleotide sequence encodes a polypeptide having heparanase catalytic activity.  
     
     
         11 . A vector comprising a polynucleotide sequence encoding a polypeptide having heparanase catalytic activity, wherein said polypeptide shares at least 70% homology with SEQ ID NOs:10, 14 or 44, as determined using default parameters of a DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the University of Wisconsin.  
     
     
         12 . The vector of  claim 11 , wherein said polynucleotide sequence includes nucleotides 63-1691 of SEQ ID NO:9.  
     
     
         13 . The vector of  claim 11 , wherein said polynucleotide sequence includes nucleotides 63-721 of SEQ ID NO:9.  
     
     
         14 . The vector of  claim 11 , wherein said polynucleotide sequence is as set forth in SEQ ID NO:9.  
     
     
         15 . The vector of  claim 11 , wherein said polynucleotide sequence includes a segment of SEQ ID NO:9, said segment encodes said polypeptide having said heparanase catalytic activity.  
     
     
         16 . The vector of  claim 11 , wherein said polypeptide includes an amino acid sequence as set forth in SEQ ID NOs:10, 14 or 44.  
     
     
         17 . The vector of  claim 11 , wherein said polypeptide includes a segment of SEQ ID NOs:10, 14 or 44, said segment harbors said heparanase catalytic activity.  
     
     
         18 . The vector of  claim 11 , wherein said polynucleotide sequence is selected from the group consisting of double stranded DNA, single stranded DNA and RNA.  
     
     
         19 . The vector of  claim 11 , wherein said vector is a baculovirus vector.  
     
     
         20 . A host cell comprising an exogenous polynucleotide fragment including a polynucleotide sequence encoding a polypeptide having heparanase catalytic activity, wherein said polypeptide shares at least 70% homology with SEQ ID NOs:10, 14 or 44, as determined using default parameters of a DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the University of Wisconsin.  
     
     
         21 . The host cell of  claim 20 , wherein said polynucleotide sequence includes nucleotides 63-1691 of SEQ ID NO:9.  
     
     
         22 . The host cell of  claim 20 , wherein said polynucleotide sequence includes nucleotides 63-721 of SEQ ID NO:9.  
     
     
         23 . The host cell of  claim 20 , wherein said polynucleotide sequence is as set forth in SEQ ID NO:9.  
     
     
         24 . The host cell of  claim 20 , wherein said polynucleotide sequence includes a segment of SEQ ID NO:9, said segment encodes said polypeptide having said heparanase catalytic activity.  
     
     
         25 . The host cell of  claim 20 , wherein said polypeptide includes an amino acid sequence as set forth in SEQ ID NOs:10, 14 or 44.  
     
     
         26 . The host cell of  claim 20 , wherein said polypeptide includes a segment of SEQ ID NOs:10, 14 or 44 said segment harbors said heparanase catalytic activity.  
     
     
         27 . The host cell of  claim 20 , wherein said polynucleotide sequence is selected from the group consisting of double stranded DNA, single stranded DNA and RNA.  
     
     
         28 . A host cell expressing a recombinant heparanase, wherein said recombinant heparanase shares at least 70% homology with SEQ ID NOs:10, 14 or 44, as determined using default parameter of a DNA sequence analysis software package developed by the Genetic Computer (Group (GCG) at the University of Wisconsin.  
     
     
         29 . A heparanase overexpression system comprising a cell overexpressing heparanase catalytic activity, wherein said heparanase catalytic activity is effected by a heparanase sharing at least 70% homology with SEQ ID NOs:10, 14 or 44, as determined using default parameters of a DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the University of Wisconsin.  
     
     
         30 . The host cell of  claim 20 , wherein said cell is an insect cell.

Join the waitlist — get patent alerts

Track US2004142427A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.