Nucleic acid encoding human G protein-coupled receptor

Abstract

The invention disclosed in this patent document relates to transmembrane receptors, more particularly to endogenous, human orphan G protein-coupled receptors. The invention provides, in part, polynucleotides encoding the endogenous, human orphan G protein-coupled receptors.

What is claimed is:  
     
         1 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor comprising an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         2 . The method of  claim 1  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor comprising an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530.  
     
     
         3 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor consisting of an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, comprising the steps of: 
 (c) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (d) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         4 . The method of  claim 3  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor consisting of an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530.  
     
     
         5 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor comprising the polypeptide of SEQ ID NO:20, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         6 . The method of  claim 5  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor, said receptor comprising the polypeptide of SEQ ID NO:20.  
     
     
         7 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor consisting of the polypeptide of SEQ.ID.NO.:20, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         8 . The method of  claim 7  wherein said host cell comprises an expression vector, said expression vector comprising a polynucelotide encoding a G protein-coupled receptor, said receptor consisting of the polypeptide of SEQ.ID.NO.:20.  
     
     
         9 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor comprising an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, and wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is substituted with another amino acid, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         10 . The method of  claim 9  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor comprising an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, and wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is substituted with another amino acid.  
     
     
         11 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor consisting of an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, and wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is substituted with another amino acid, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         12 . The method of  claim 11  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor consisting of an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, and wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is substituted with another amino acid.  
     
     
         13 . A method of  claim 9  wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is glycine and wherein the glycine at said amino acid position 285 is substituted with an amino acid other than glycine.  
     
     
         14 . The method of  claim 13  wherein said amino acid other than glycine is lysine.  
     
     
         15 . The method of  claim 13  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor comprising an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is glycine and wherein the glycine at said amino acid position 285 is substituted with an amino acid other than glycine.  
     
     
         16 . The method of  claim 15  wherein said amino acid other than glycine is lysine.  
     
     
         17 . A method of  claim 11  wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is glycine and wherein the glycine at said amino acid position 285 is substituted with an amino acid other than glycine.  
     
     
         18 . The method of  claim 17  wherein said amino acid other than glycine is lysine.  
     
     
         19 . The method of  claim 17  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor consisting of an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization, under stringent conditions, on a sample of human DNA library using specific probe EST clone 68530, wherein the amino acid at amino acid position 285 of said endogenous human ARE-2 polypeptide is glycine and wherein the glycine at said amino acid position 285 is substituted with an amino acid other than glycine.  
     
     
         20 . The method of  claim 19  wherein said amino acid other than glycine is lysine.  
     
     
         21 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor comprising the polypeptide of SEQ ID NO:20, wherein the glycine at amino acid position 285 of SEQ ID NO:20 is substituted with an amino acid other than glycine, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         22 . The method of  claim 21  wherein the glycine at amino acid position 285 is substituted with lysine.  
     
     
         23 . The method of  claim 21  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor comprising the polypeptide of SEQ ID NO:20, wherein the glycine at amino acid position 285 of SEQ ID NO:20 is substituted with an amino acid other than glycine.  
     
     
         24 . A method for identifying one or more candidate compounds as modulators of a G protein-coupled receptor consisting of the polypeptide of SEQ ID NO:20, wherein the glycine at amino acid position 285 of SEQ ID NO:20 is substituted with an amino acid other than glycine, comprising the steps of: 
 (a) contacting said one or more compounds with a host cell or with membrane of a host cell that expresses said receptor; and    (b) measuring the ability of the compound or compounds to inhibit or stimulate functionality of said receptor.    
     
     
         25 . The method of  claim 24  wherein the glycine at amino acid position 285 is substituted with lysine.  
     
     
         26 . The method of  claim 24  wherein said host cell comprises an expression vector, said expression vector comprising a polynucleotide encoding a G protein-coupled receptor consisting of the polypeptide of SEQ ID NO:20, wherein the glycine at amino acid position 285 of SEQ ID NO:20 is substituted with an amino acid other than glycine.  
     
     
         27 . A method of modulating the functionality of a G protein-coupled receptor comprising an endogenous human ARE-2 polypeptide, wherein said endogenous human ARE-2 polypeptide is encoded by a nucleotide sequence, said nucleotide sequence being obtainable by performing nucleic acid hybridization on a sample of human genomic DNA using specific probe EST clone 68530, comprising the step of contacting the receptor with a modulator of the receptor.