US2004127685A1PendingUtilityA1

Novel molecules of the card-related protein family and uses thereof

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Assignee: MILLENNIUM PHARM INCPriority: Jun 28, 1999Filed: Jan 13, 2004Published: Jul 1, 2004
Est. expiryJun 28, 2019(expired)· nominal 20-yr term from priority
Inventors:John Bertin
C07K 14/4702
60
PatentIndex Score
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Cited by
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References
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Claims

Abstract

Novel CARD-5 polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to CARD-5 proteins, and the invention further provides CARD-5 fusion proteins, antigenic peptides and anti-CARD-5 antibodies. The invention also provides CARD-5 nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a CARD-5 gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . An isolated polypeptide comprising amino acids 1-88 or 111-195 of SEQ ID No:5.  
     
     
         2 . The isolated polypeptide of  claim 1 , wherein the polypeptide comprises amino acids 1-88 of SEQ ID NO:5.  
     
     
         3 . The isolated polypeptide of  claim 1 , wherein the polypeptide comprises amino acids 111-195 of SEQ ID NO:5.  
     
     
         4 . The isolated polypeptide of  claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:5.  
     
     
         5 . The isolated polypeptide of  claim 1 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO:5.  
     
     
         6 . An isolated polypeptide comprising an amino acid sequence that is at least 85% identical to SEQ ID NO:5, wherein the polypeptide stimulates NF-kB activity.  
     
     
         7 . The polypeptide of  claim 6 , wherein the amino acid sequence is at least 95% identical to SEQ ID NO:5.  
     
     
         8 . The polypeptide of  claim 6 , wherein the amino acid sequence is at least 98% identical to SEQ ID NO:5.  
     
     
         9 . An isolated polypeptide comprising an amino acid sequence that is at least 85% identical to SEQ ID NO:5, wherein the polypeptide binds to caspase-1, CARD-7, CARD-12, or CARD-5.  
     
     
         10 . The polypeptide of  claim 9 , wherein the amino acid sequence is at least 95% identical to SEQ ID NO:5.  
     
     
         11 . The polypeptide of  claim 9 , wherein the amino acid sequence is at least 98% identical to SEQ ID NO:5.  
     
     
         12 . The polypeptide of  claim 9 , wherein the polypeptide binds to caspase-1.  
     
     
         13 . The polypeptide of  claim 9 , wherein the polypeptide binds to CARD-7.  
     
     
         14 . The polypeptide of  claim 9 , wherein the polypeptide binds to CARD-12.  
     
     
         15 . The polypeptide of  claim 9 , wherein the polypeptide binds to CARD-5.  
     
     
         16 . An isolated polypeptide comprising an amino acid sequence that is at least 85% identical to SEQ ID NO:5, wherein the polypeptide induces apoptosis.  
     
     
         17 . The polypeptide of  claim 12 , wherein the amino acid sequence is at least 95% identical to SEQ ID NO:5.  
     
     
         18 . The polypeptide of  claim 12 , wherein the amino acid sequence is at least 98% identical to SEQ ID NO:5.  
     
     
         19 . An isolated polypeptide comprising an amino acid sequence that is at least 90% identical to amino acids 111-195 of SEQ ID NO:5, wherein the polypeptide binds to caspase-1, CARD-7, CARD-12, or CARD-5.  
     
     
         20 . The polypeptide of  claim 19 , wherein the amino acid sequence is at least 95% identical to amino acids 111-195 of SEQ ID NO:5.  
     
     
         21 . The polypeptide of  claim 19 , wherein the amino acid sequence is at least 98% identical to amino acids 111-195 of SEQ ID NO:5.  
     
     
         22 . The polypeptide of  claim 19 , wherein the polypeptide binds to caspase-1.  
     
     
         23 . The polypeptide of  claim 19 , wherein the polypeptide binds to CARD-7.  
     
     
         24 . The polypeptide of  claim 19 , wherein the polypeptide binds to CARD-12.  
     
     
         25 . The polypeptide of  claim 19 , wherein the polypeptide binds to CARD-5.  
     
     
         26 . An isolated polypeptide comprising an amino acid sequence encoded by the cDNA insert of the plasmid EpHC5 deposited with the ATCC as Accession Number PTA-213.  
     
     
         27 . A fusion protein comprising the polypeptide of  claim 2  linked by a peptide bond to a heterologous polypeptide.  
     
     
         28 . A fusion protein comprising the polypeptide of  claim 3  linked by a peptide bond to a heterologous polypeptide.  
     
     
         29 . A fusion protein comprising the polypeptide of  claim 4  linked by a peptide bond to a heterologous polypeptide.  
     
     
         30 . A fusion protein comprising the polypeptide of  claim 6  linked by a peptide bond to a heterologous polypeptide.  
     
     
         31 . A fusion protein comprising the polypeptide of  claim 9  linked by a peptide bond to a heterologous polypeptide.  
     
     
         32 . A fusion protein comprising the polypeptide of  claim 16  linked by a peptide bond to a heterologous polypeptide.  
     
     
         33 . A fusion protein comprising the polypeptide of  claim 19  linked by a peptide bond to a heterologous polypeptide.  
     
     
         34 . A fusion protein comprising the polypeptide of  claim 26  linked by a peptide bond to a heterologous polypeptide.

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