Antibodies active against a fusion polypeptide comprising a histidine portion

Abstract

The present invention relates to an antibody active against a fusion polypeptide comprising a histidine portion, a process for the preparation thereof and its use.

1 . An antibody against a fusion polypeptide comprising a histidine portion.  
     
     
         2 . The antibody according to  claim 1 , characterized in that it is polyclonal.  
     
     
         3 . The antibody according to  claim 1 , characterized in that it is monoclonal.  
     
     
         4 . The antibody according to  claim 3 , characterized in that it is deposited under ACC 2207 with DSM [German-type culture collection for microorganisms].  
     
     
         5 . A process for the preparation of an antibody according to any one of  claims 1  to  4 , characterized in that an animal is immunized with a histidine fusion polypeptide and 
 (a) polyclonal antibodies are obtained from the serum of the animal, or  
 (b) monoclonal antibodies are obtained after the fusion of animals spleen cells with myeloma cells.  
 
     
     
         6 . The process according to  claim 5 , characterized in that a mixture of histidine fusion polypeptides is used for immunization.  
     
     
         7 . Use of an antibody according to any one of  claims 1  to  4  in a detection method for a fusion polypeptide comprising a histidine portion.  
     
     
         8 . Use according to  claim 7 , wherein the detection method is a Western blot, an ELISA, an immunofluorescence or an immunoprecipitation.