Well frame including connectors for biological fluids

Abstract

A device, a kit and a method for the hybridization reactions between biomolecules are disclosed. The invention relates to a hybridization chamber including a generally planar substrate which includes a specimen area containing at least one biomolecule. The hybridization chamber also includes a frame surrounding at least a portion of the specimen area. The frame defines walls of a well for holding a fluid when the frame is in contact with the substrate. The frame includes a connector adapted to connect to tubing for supplying fluids to the well. Tubing connected to the connectors may be used to supply wash fluid and hybridization fluid, and waste tubing may be connected to a second connector for removing waste material from the well.

What is claimed is:  
     
         1 . A kit for performing a binding assay comprising: 
 a hybridization chamber having a generally planar substrate including a specimen area containing at least one biomolecule; and    a frame surrounding at least a portion of the specimen area, the frame defining walls of a well for containing a fluid when the frame is in contact with the substrate, the frame including a connector adapted to connect to tubing for supplying fluids to the well, wherein the connector is integrally formed with said frame and selected from the group consisting of male luer fittings, female luer fittings, flanged fittings, flangeless fittings, threaded fittings and barbed fittings and said frame is self-adhering to said substrate.    
     
     
         2 . The kit according to  claim 1 , wherein the kit further includes a slide for printing a high-density microarray of biomolecules.  
     
     
         3 . The kit according to  claim 1 , wherein the kit further includes a microarray of nucleic acid.