5-Lipoxygenase-activating protein II

Abstract

Disclosed is a human FLAP II polypeptide and DNA (RNA) encoding such polypeptide. Also provided is a procedure for producing such polypeptide by recombinant techniques. Further, antagonists against such polypeptide are disclosed. Such antagonists may be used for therapeutic proposes, for example, for treating inflamation, bronchial asthma and may also be used as gastric cytoprotective agents and to treat human glomerulonephritis. Diagnostic assays for identifying mutations in nucleic acid sequences encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention are also disclosed.

What is claimed is:  
     
         1 . An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of: 
 (a) a protein consisting of amino acid residues 1 to 147 of SEQ ID NO:2;    (b) a protein consisting of amino acid residues 2 to 147 of SEQ ID NO:2;    (c) a protein consisting of the full-length polypeptide encoded by the cDNA contained in ATCC Deposit Number 75771; and    (d) a protein consisting of the full-length polypeptide encoded by the cDNA contained in the ATCC Deposit Number 75771, minus the N-terminal methionine.    
     
     
         2 . The antibody or fragment thereof of  claim 1  that specifically binds protein (a).  
     
     
         3 . The antibody or fragment thereof of  claim 1  that specifically binds protein (b).  
     
     
         4 . The antibody or fragment thereof of  claim 2  that specifically binds protein (b).  
     
     
         5 . The antibody or fragment thereof of  claim 1  that specifically binds protein (c).  
     
     
         6 . The antibody or fragment thereof of  claim 1  that specifically binds protein (d).  
     
     
         7 . The antibody or fragment thereof of  claim 5  that specifically binds protein (b).  
     
     
         8 . The antibody or fragment thereof of  claim 1 , which is a monoclonal antibody.  
     
     
         9 . The antibody or fragment thereof of  claim 1  wherein said antibody or fragment thereof specifically binds to said protein in a Western blot.  
     
     
         10 . The antibody or fragment thereof of  claim 1  wherein said antibody or fragment thereof specifically binds to said protein in an ELISA.