Ostertagia vaccine

Abstract

The present invention relates to nucleic acid sequences encoding Ostertagia ostertagi proteins and to parts of such nucleic acid sequences that encode an immunogenic fragment of such proteins, and to DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof; to Ostertagia ostertagi proteins and immunogenic parts thereof encoded by such sequences; to vaccines comprising such nucleic acid sequences and parts thereof, DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof, proteins or immunogenic parts thereof and antibodies against such proteins or immunogenic parts thereof; to the use of said proteins in vaccines and for the manufacture of vaccines; to the use of said nucleic acid sequences, proteins or antibodies for diagnostic or vaccination purposes; and to diagnostic kits comprising such nucleic acids, proteins or antibodies against such proteins.

We claim:  
     
         1 . A nucleic acid sequence encoding an  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 1.  
     
     
         2 . A nucleic acid sequence encoding a 28 kD  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 3.  
     
     
         3 . A nucleic acid sequence encoding a 25 kD  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 5.  
     
     
         4 . A nucleic acid sequence encoding a 31 kD  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 7.  
     
     
         5 . A nucleic acid sequence encoding a 30 kD  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 9.  
     
     
         6 . A nucleic acid sequence encoding a 24 kD  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 11.  
     
     
         7 . A nucleic acid sequence encoding a 65 kD  Ostertagia ostertagi  protein or a part of said nucleic acid sequence that encodes an immunogenic fragment of said protein, said nucleic acid sequence or said part thereof having at least 85% homology with the nucleic acid sequence of the  Ostertagia ostertagi  protein gene as depicted in SEQ ID NO: 13.  
     
     
         8 . A DNA fragment, comprising: 
 a nucleic acid sequence according to  claim 1 .    
     
     
         9 . A recombinant DNA molecule comprising a nucleic acid sequence according to  claim 1 , under the control of a functionally linked promoter.  
     
     
         10 . A live recombinant carrier comprising a nucleic acid sequence according to  claim 1 .  
     
     
         11 . A host cell comprising a nucleic acid sequence according to  claim 1 .  
     
     
         12 . An  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 2.  
     
     
         13 . A 28 kD  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 4.  
     
     
         14 . A 25 kD  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 6.  
     
     
         15 . A 31 kD  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 8.  
     
     
         16 . A 30 kD  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 10.  
     
     
         17 . A 24 kD  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 12.  
     
     
         18 . A 65 kD  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment thereof has a sequence homology of at least 90% to the amino acid sequence as depicted in SEQ ID NO: 14.  
     
     
         19 . An  Ostertagia ostertagi  protein or an immunogenic fragment of said protein, wherein said protein or immunogenic fragment is encoded by a nucleic acid sequence according to  claim 1 .  
     
     
         20 . A vaccine for combating  Ostertagia ostertagi  infection, wherein said vaccine comprises at least one  Ostertagia ostertagi  protein or an immunogenic fragment of said protein according to  claim 12  and a pharmaceutically acceptable carrier.  
     
     
         21 . A vaccine for combating  Ostertagia ostertagi  infection, wherein said vaccine comprises a nucleic acid sequence according to claims  1 , and a pharmaceutically acceptable carrier.  
     
     
         22 . A vaccine for combating  Ostertagia ostertagi  infection, wherein said vaccine comprises a live recombinant carrier according to  claim 10  and a pharmaceutically acceptable carrier.  
     
     
         23 . A vaccine for combating  Ostertagia ostertagi  infection, wherein said vaccine comprises a host cell according to  claim 11  and a pharmaceutically acceptable carrier.  
     
     
         24 . A vaccine for combating  Ostertagia ostertagi  infection, wherein said vaccine comprises antibodies against a protein or an immunogenic fragment thereof according to  claim 12  and a pharmaceutically acceptable carrier.  
     
     
         25 . The vaccine according to  claim 20 , wherein said vaccine comprises an adjuvant.  
     
     
         26 . The vaccine according to  claim 20 , wherein said vaccine comprises an additional antigen derived from a virus or micro-organism pathogenic to cattle, an antibody against said antigen or genetic information encoding said antigen and/or a pharmaceutical component.  
     
     
         27 . The vaccine according to  claim 26 , wherein said virus or microorganism pathogenic to cattle is selected from the group of Bovine Herpesvirus, bovine Viral Diarrhea virus, Parainfluenza type 3 virus, Bovine Paramyxovirus, Foot and Mouth Disease virus,  Pasteurella haemolytica , Bovine Respiratory Syncytial Virus, Theileria sp., Babesia sp., Trypanosoma species, Anaplasma sp.,  Neospora caninum, Staphylococcus aureus, Streptococcus agalactiae , Mycoplasma,  E. coli , Enterobacter, Klebsiella, Citrobacter and  Streptococcus dysgalactiae.    
     
     
         28 . A diagnostic kit, comprising: 
 suitable detection means and    a nucleic acid sequence according to claims  1 .    
     
     
         29 . A diagnostic kit, comprising: 
 suitable detection means and    a protein or immunogenic fragment thereof according to  claim 12 .    
     
     
         30 . A diagnostic kit, comprising: 
 suitable detection means and    antibodies that are reactive with a protein according to  claim 12 .    
     
     
         31 . A method of preventing or treating an infection caused by  Ostertagia ostertagi  in an animal, comprising: 
 administering an effective amount of the vaccine according  claim 20  to the animal.    
     
     
         32 . A method of preventing or treating an infection caused by  Ostertagia ostertagi  in an animal, comprising: 
 administering an effective amount of the vaccine according  claim 21  to the animal.    
     
     
         33 . A method of preventing or treating an infection caused by  Ostertagia ostertagi  in an animal, comprising: 
 administering an effective amount of the vaccine according  claim 22  to the animal.    
     
     
         34 . A method of preventing or treating an infection caused by  Ostertagia ostertagi  in an animal, comprising: 
 administering an effective amount of the vaccine according  claim 23  to the animal.    
     
     
         35 . A method of preventing or treating an infection caused by  Ostertagia ostertagi  in an animal, comprising: 
 administering an effective amount of the vaccine according  claim 24  to the animal.