Identification of a region of the major surface glycoprotein (MSG) gene of human Pneumocystis carinii
Abstract
Particularly sensitive techniques for the detection of P. carinii in clinical samples are disclosed. These techniques relate to the PCR amplification and/or detection of human- P. carinii major surface glycoprotein (MSG) gene sequences. Also disclosed are seven novel genes encoding human- P. carinii MSG, and the proteins encoded for by these genes. These genes provide proof that human- P. carinii MSG is encoded for by a highly conserved gene family, and that the corresponding proteins have a very highly conserved region of about 100 amino acids near their C-terminal end. This highly conserved carboxy-terminal region has a significantly different sequence than that found in rat-derived MSG.
Claims
exact text as granted — not AI-modifiedWe claim:
1 . An isolated nucleic acid molecule encoding an amino acid sequence as set forth in SEQ ID NO: 14.
2 . The isolated nucleic acid molecule according to claim 1 , wherein the nucleic acid molecule consists of a sequence as set forth in SEQ ID NO: 13.
3 . A recombinant vector comprising the nucleic acid molecule according to claim 1 .
4 . A transgenic cell comprising the vector according to claim 3 .
5 . An isolated nucleic acid molecule comprising a sequence as set forth in SEQ ID NO: 13.
6 . A recombinant vector comprising the nucleic acid molecule according to claim 5 .
7 . A transgenic cell comprising the vector according to claim 6 .
8 . A kit for detecting a human- P. carinii nucleic acid sequence comprising:
a control nucleic acid sequence as set forth in SEQ ID NO: 13, and at least one oligonucleotide primer comprising a sequence selected from the group consisting of SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; and SEQ ID NO: 24.
9 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 17.
10 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 18.
11 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 19.
12 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 21.
13 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence of SEQ ID NO: 22.
14 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 23.
15 . The kit of claim 8 , wherein one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 24.
16 . A kit for detecting a human- P. carinii nucleic acid sequence comprising:
a control nucleic acid sequence as set forth in SEQ ID NO: 13, and at least a pair of oligonucleotide primers each comprising at least 15 contiguous nucleotides of SEQ ID NO: 13.
17 . The kit of claim 16 , wherein at least one of the oligonucleotide primers comprises a sequence selected from the group consisting of SEQ ID NO: 17; SEQ ID NO: 19; and SEQ ID NO: 20.
18 . The kit of claim 16 , wherein at least one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 17.
19 . The kit of claim 16 , wherein at least one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 19.
20 . The kit of claim 16 , at least one of the oligonucleotide primers comprises the sequence as set forth in SEQ ID NO: 20.Join the waitlist — get patent alerts
Track US2004043409A1 — get alerts on status changes and closely related new filings.
We store only your email — no account needed. See our privacy policy.