8-oxyguanine DNA glycosylase polypeptides

Abstract

The invention provides isolated polynucleotides encoding polypeptides having 8-oxoguanine DNA glycosylase (OGG) activity. The present invention provides methods and compositions relating to expressing OGG in plants in order to improve transformation efficiency, homologous recombination and/or targeted gene modifications. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

What is claimed is  
     
         1 . An isolated protein comprising a full-length polypeptide comprising at least 30 contiguous amino acids from the polypeptide of SEQ ID NO: 2, wherein the polypeptide has 8-oxyguanine DNA glycosylase (OGG) activity.  
     
     
         2 . An isolated protein comprising an amino acid sequence having at least 75% sequence identity over the entire length of SEQ ID NO: 2; wherein the percent identity is determined by the GAP algorithm under default parameters, and wherein said sequence encodes a polypeptide having 8-oxyguanine DNA glycosylase (OGG) activity.  
     
     
         3 . The isolated protein of  claim 2 , wherein the amino acid sequence has at least 80% sequence identity over the entire length of SEQ ID NO: 2.  
     
     
         4 . The isolated protein of  claim 2 , wherein the amino acid sequence has at least 85% sequence identity over the entire length of SEQ ID NO: 2.  
     
     
         5 . The isolated protein of  claim 2 , wherein the amino acid sequence has at least 90% sequence identity over the entire length of SEQ ID NO: 2.  
     
     
         6 . The isolated protein of  claim 2 , wherein the amino acid sequence has at least 95% sequence identity over the entire length of SEQ ID NO: 2.  
     
     
         7 . An isolated protein comprising a polypeptide encoded by SEQ ID NO: 1, wherein the protein has 8-oxyguanine DNA glycosylase (OGG) activity.  
     
     
         8 . An isolated protein comprising the polypeptide of SEQ ID NO: 2, wherein the protein has 8-oxyguanine DNA glycosylase (OGG) activity.