Transformation system in camelina sativa

Abstract

The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

1 . A method for Agrobacterium-mediated genetic transformation, characterized in that the method is Agrobacterium-mediated genetic transformation of  Camelina sativa  comprising the steps of: 
 (a) providing explants from  Camelina sativa;      (b) contacting the explants of  Camelina sativa  with Agrobacterium containing at least one recombinant DNA construct;    (c) allowing the transformation to take place on culture medium optionally supplemented with at least one hormone;    (d) inducing formation (regeneration) of one or more shoots and roots from the transformed explants on a cell culture medium optionally containing at least one hormone; and    (e) growing the shoots into a whole  Camelina sativa  plant.    
     
     
         2 . The method according to  claim 1 , characterized in that the explants are obtained from  Camelina sativa  plants grown from optionally sterilized seeds.  
     
     
         3 . The method according to  claim 1 , characterized in that the Agrobacterium is  Agrobacterium tumefaciens.    
     
     
         4 . The method according to  claim 1 , characterized in that the recombinant DNA construct is a vector comprising an optional selectable marker gene.  
     
     
         5 . The method according to  claim 1 , characterized in that transformed tissue of  Camelina sativa  is optionally selected on a medium containing a selective component.  
     
     
         6 . The method according to  claim 1 , characterized in that the  Camelina sativa  explant is selected from hypocotyl, cotyledon, stem, leaf or other plant organs.  
     
     
         7 . The method according to  claim 1 , characterized in that the selectable marker gene comprises antibiotic resistance genes or mixtures therof.  
     
     
         8 . The method according to  claim 7 , characterized in that the antibiotic resistance gene comprises neomycin phosphotransferase (npt II) gene or hygromycin phosphotransferase (hpt) gene or mixtures thereof.  
     
     
         9 . The method according to  claim 1 , characterized in that the selective component is antibiotic.  
     
     
         10 . The method according to  claim 9 , characterized in that the antibiotic is hygromycin or kanamycin.  
     
     
         11 . The method according to  claim 1 , characterized in that the induction of the shoots and roots comprises application of hormone or a growth factor allowing regeneration.  
     
     
         12 . The method according to  claim 1 , characterized in that the recombinant DNA construct is a DNA sequence comprising at least one gene of interest, at least one promoter sequence and an optional selectable marker gene.  
     
     
         13 . The method according to  claim 1 , characterized in that the optional hormone is cytokinin, auxin, gibberellin or mixtures thereof.  
     
     
         14 . The method according to  claim 13 , characterized in that the cytokinin comprises 6-benzylaminopurine (BAP), kinetin, zeatin, zeatin riboside, dihydrozeatin, isopentenyl adenine or mixtures thereof.  
     
     
         15 . The method according to  claim 13 , characterized in that the auxin comprises Â-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), 4-chloro-IAA, phenylacetic acid, 2,4-dichlorophenoxyacetic acid (2,4-D) or mixtures thereof.  
     
     
         16 . The method according to claims  1 - 15  for obtaining a transgenic  Camelina sativa  plant.  
     
     
         17 . The method according to claims  1 - 15  for obtaining transgenic  Camelina sativa  plant tissue.  
     
     
         18 . The method according to claims  1 - 15  for obtaining transgenic  Camelina sativa  plant cells and cell lines.  
     
     
         19 . A transgenic plant, characterized in that it comprises a  Camelina sativa  plant obtainable with the method of  claim 1 .  
     
     
         20 . A transgenic plant tissue, characterized in that it comprises  Camelina sativa  tissue obtainable with the method of  claim 1 .  
     
     
         21 . A transgenic plant cell or cell lines, characterized in that it comprises  Camelina sativa  cells or cell lines obtainable with the method of  claim 1 .  
     
     
         22 . Transgenic seed, characterized in that it comprises  Camelina sativa  seed obtainable with the method of  claim 1 .  
     
     
         23 . The use of the method according to any of claims  1 - 15  for providing a transformation system in  Camelina sativa.    
     
     
         24 . The use of the method according to any of claims  1 - 15  to produce homologous or heterologous recombinant products in  Camelina sativa.    
     
     
         25 . The use of the method according to  claim 24  where the homologous or heterologous products are proteins of metabolites.  
     
     
         26 . The use of the method according to any of claims  1 - 15  for producing oil products with unique properties in  Camelina sativa.    
     
     
         27 . The use of  Camelina sativa  as an alternative model plant in Agrobacterium-mediated transformation.