Cell cycle control protein

Abstract

A DNA containing a nucleotide sequence encoding the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing, or a nucleotide sequence encoding a protein having the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing with partial substitution, deletion or addition and having cell cycle control activity, or a nucleotide sequence hybridizing to them; a recombinant vector containing said DNA; a cell transformed with said vector; a process for producing AIM-1 using said DNA; a recombinant AIM-1 protein obtained by said process; an oligonucleotide or peptide nucleic acid capable of specifically hybridizing to a nucleotide sequence encoding AIM-1 protein; an antibody recognizing AIM-1; a therapeutic agent for diseases associated with abnormal cell growth containing an inhibitor against AIM-1 protein; and a screening method for materials having serine-threonine inhibitory activity using AIM-1 gene or AIM-1 protein.

What is claimed is:  
     
         1 . A DNA containing a nucleotide sequence encoding the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing, or a nucleotide sequence encoding a protein having the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing with partial substitution, deletion or addition and having cell cycle control activity, or a nucleotide sequence hybridizing to them.  
     
     
         2 . A recombinant vector containing the DNA of  claim 1 .  
     
     
         3 . A prokaryotic or eukaryotic host cell transformed with the recombinant vector of  claim 2 .  
     
     
         4 . A process for producing a recombinant protein, comprising culturing the host cell of  claim 3 , and isolating and purifying the protein produced.  
     
     
         5 . The process of  claim 4  wherein the recombinant protein has cell cycle control activity.  
     
     
         6 . A recombinant AIM-1 protein obtained by isolating and purifying the culture supernatant obtained by culturing the host cell of  claim 3 .  
     
     
         7 . An oligonucleotide or peptide nucleic acid capable of specifically hybridizing to a nucleotide sequence encoding the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing.  
     
     
         8 . An oligonucleotide or peptide nucleic acid capable of specifically hybridizing to a nucleotide sequence encoding the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing and inhibiting expression of AIM-1 protein.  
     
     
         9 . An antibody recognizing a peptide having at least five continuous amino acids in the amino acid sequence shown as SEQ ID NO: 2 in Sequence Listing.  
     
     
         10 . A therapeutic agent for diseases associated with abnormal cell growth, containing an inhibitor against AIM-1 protein.  
     
     
         11 . The therapeutic agent of  claim 10  wherein the inhibitor against AIM-1 protein is AIM-1 (K-R), AIM-1 protein kinase activity inhibitor, the oligonucleotide or peptide nucleic acid of  claim 7 , or the antibody of  claim 8 .  
     
     
         12 . The therapeutic agent of  claim 11  wherein the disease associated with abnormal cell growth is cancer.  
     
     
         13 . A screening method for materials having serine-threonine inhibitory activity using AIM-1 gene or AIM-1 protein.