Motor proteins and methods for their use

Abstract

The invention provides isolated nucleic acid and amino acid sequences of HsKip3d, antibodies to HsKip3d, methods of screening for HsKip3d modulators using biologically active HsKip3d, and kits for screening for HsKip3d modulators.

What is claimed is:  
     
         1 . An isolated nucleic acid sequence encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule stimulated ATPase activity; and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.  
     
     
         2 . An isolated nucleic acid sequence of  claim 1 , wherein the protein specifically binds to polyclonal antibodies to a protein comprising SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         3 . An isolated nucleic acid sequence of  claim 1 , wherein the nucleic acid encodes SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         4 . An isolated nucleic acid sequence of  claim 1 , wherein the nucleic acid has a nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:3.  
     
     
         5 . An isolated nucleic acid sequence of  claim 1 , wherein the nucleic acid selectively hybridizes under stringent hybridization conditions to SEQ ID NO:1 or SEQ ID NQ:3.  
     
     
         6 . An expression vector comprising a nucleic acid encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule stimulated ATPase activity, and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.  
     
     
         7 . A host cell transfected with the vector of  claim 6 .  
     
     
         8 . An isolated microtubule motor protein, wherein the protein has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.  
     
     
         9 . An isolated protein of  claim 8 , wherein the protein specifically binds to polyclonal antibodies to HsKip3d.  
     
     
         10 . An isolated protein of  claim 8 , wherein the protein is HsKip3d.  
     
     
         11 . An isolated protein of  claim 8 , wherein the protein has an amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         12 . An isolated protein of  claim 8 , wherein the protein specifically binds to polyclonal antibodies generated against a motor domain of HsKip3d.  
     
     
         13 . An isolated protein of  claim 8 , wherein the protein comprises an amino acid sequence of a HsKip3d motor domain.  
     
     
         14 . A method for screening for modulators of HsKip3d, the method comprising the steps of: 
 (i) providing biologically active HsKip3d, wherein the HsKip3d has the following properties: (i) activity including microtubule stimulated ATPase activity; and (ii) sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm;    (ii) contacting biologically active HsKip3d with a candidate agent in a test and control concentration; and    (iii) assaying for the level of HsKip3d activity, wherein the HsKip3d activity is selected from the group consisting of binding activity or ATPase activity, and wherein a change in activity between the test and control concentration indicates a modulator.    
     
     
         15 . A method of  claim 14 , wherein the screening occurs in a multi-well plate as part of a high-throughput screen.  
     
     
         16 . A method of  claim 14 , wherein the biologically active HsKip3d comprises an amino acid sequence of a HsKip3d motor domain.  
     
     
         17 . A compound that modulates HsKip3d, wherein said compound is identified using the method of  claim 14 .  
     
     
         18 . An isolated nucleic acid comprising a sequence which has greater than 60% sequence identity with nucleotide SEQ ID NO:1 or SEQ ID NO:3.