US2004018982A1PendingUtilityA1

Nematicidal proteins

64
Priority: Aug 12, 1987Filed: Jul 31, 2003Published: Jan 29, 2004
Est. expiryAug 12, 2007(expired)· nominal 20-yr term from priority
Inventors:H. Ernest SchnepfGeorge E. SchwabJewel PayneKenneth E. NarvaLuis Foncerrada
C07K 14/325A01N 37/46C12R 2001/075C12N 1/205A01N 63/50
64
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Claims

Abstract

This invention concerns nematicidal proteins obtainable from Bacillus thuringiensis isolates. The subject invention also provides various methods of using these proteins for controlling nematodes.

Claims

exact text as granted — not AI-modified
1 . An isolated, nematicidal protein wherein said protein comprises a nematicidal portion of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:10, and SEQ ID NO:12.  
     
     
         2 . The protein of  claim 1  wherein said protein comprises a nematicidal portion of SEQ ID No:2.  
     
     
         3 . The protein of  claim 1  wherein said protein comprises a nematicidal portion of SEQ ID NO:4.  
     
     
         4 . The protein of  claim 1  wherein said protein comprises a nematicidal portion of SEQ ID NO:6.  
     
     
         5 . The protein of  claim 1  wherein said protein comprises a nematicidal portion of SEQ ID NO:10.  
     
     
         6 . The protein of  claim 1  wherein said protein comprises a nematicidal portion of SEQ ID NO:12.  
     
     
         7 . The protein of  claim 1  wherein said protein comprises the amino acid sequence of SEQ ID NO:2.  
     
     
         8 . The protein of  claim 1  wherein said protein comprises the amino acid sequence of SEQ ID NO:4.  
     
     
         9 . The protein of  claim 1  wherein said protein comprises the amino acid sequence of SEQ ID NO:6.  
     
     
         10 . The protein of  claim 1  wherein said protein comprises the amino acid sequence of SEQ ID NO:10.  
     
     
         11 . The protein of  claim 1  wherein said protein comprises the amino acid sequence of SEQ ID NO:12.  
     
     
         12 . An isolated, nematicidal protein wherein said protein comprises a nematicidal portion of a δ-endotoxin obtainable from a  Bacillus thuringiensis  isolate selected from the group consisting of PS17 (NRRL B-18243), PS33F2 (NRRL B-18244), PS63B (NRRL B-18246), and PS69D1 (NRRL B-18247).  
     
     
         13 . The protein of  claim 12  wherein said  Bacillus thuringiensis  isolate is PS17 (NRRL B-18243).  
     
     
         14 . The protein of  claim 12  wherein said  Bacillus thuringiensis  isolate is PS33F2 (NRRL B-18244).  
     
     
         15 . The protein of  claim 12  wherein said  Bacillus thuringiensis  isolate is PS63B (NRRL B-18246).  
     
     
         16 . The protein of  claim 12  wherein said  Bacillus thuringiensis  isolate is PS69D1 (NRRL B-18247).  
     
     
         17 . The protein of  claim 15  wherein said δ-endotoxin comprises the amino acid sequence of SEQ ID NO:20.  
     
     
         18 . The protein of  claim 15  wherein said δ-endotoxin comprises the amino acid sequence of SEQ ID NO:23.  
     
     
         19 . The protein of  claim 16  wherein said δ-endotoxin comprises the amino acid sequence of SEQ ID NO:21.  
     
     
         20 . The protein of  claim 14  wherein said δ-endotoxin comprises the amino acid sequence of SEQ ID NO:22.  
     
     
         21 . The protein of  claim 12  wherein said protein comprises the amino acid sequence of a δ-endotoxin obtainable from a  Bacillus thuringiensis  isolate selected from the group consisting of PS17 (NRRL B-18243), PS33F2 (NRRL B-18244), PS52A1 (NRRL B-18245), PS63B (NRRL B-18246), and PS69D1 (NRRL B-18247).  
     
     
         22 . The protein of  claim 21  wherein said  Bacillus thuringiensis  isolate is PS17 (NRRL B-18243).  
     
     
         23 . The protein of  claim 21  wherein said  Bacillus thuringiensis  isolate is PS33F2 (NRRL B-18244).  
     
     
         24 . The protein of  claim 21  wherein said  Bacillus thuringiensis  isolate is PS63B (NRRL B-18246).  
     
     
         25 . The protein of  claim 21  wherein said  Bacillus thuringiensis  isolate is PS69D1 (NRRL B-18247).  
     
     
         26 . A method for controlling a nematode pest wherein said method comprises administering a nematicidal protein to said pest so that said pest ingests said protein, wherein said protein comprises a nematicidal portion of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:10.  
     
     
         27 . The method of  claim 26  wherein said protein comprises a nematicidal portion of SEQ ID NO:2.  
     
     
         28 . The method of  claim 26  wherein said protein comprises a nematicidal portion of SEQ ID NO:4.  
     
     
         29 . The method of  claim 26  wherein said protein comprises a nematicidal portion of SEQ ID NO:6.  
     
     
         30 . The method of  claim 26  wherein said protein comprises a nematicidal portion of SEQ ID NO:10.  
     
     
         31 . The method of  claim 26  wherein said protein comprises the amino acid sequence of SEQ ID NO:2.  
     
     
         32 . The method of  claim 26  wherein said protein comprises the amino acid sequence of SEQ ID NO:4.  
     
     
         33 . The method of  claim 26  wherein said protein comprises the amino acid sequence of SEQ ID NO:6.  
     
     
         34 . The method of  claim 26  wherein said protein comprises the amino acid sequence of SEQ ID NO:10.  
     
     
         35 . A method for controlling a nematode pest wherein said method comprises administering a nematicidal protein to said pest so that said pest ingests said protein, wherein said protein comprises a nematicidal portion of a δ-endotoxin obtainable from a  Bacillus thuringiensis  isolate selected from the group consisting of PS33F2 (NRRL B-18244), PS52A1 (NRRL B-18245), and PS69D1 (NRRL B-18247), wherein said δ-endotoxin comprises SEQ ID NO:22 when said isolate is PS33F2, said δ-endotoxin comprises SEQ ID NO:19 when said isolate is PS52A1, and said δ-endotoxin comprises SEQ ID NO:21 when said isolate is PS69D1.  
     
     
         36 . The method of  claim 35  wherein said isolate is PS33F2 and said δ-endotoxin comprises SEQ ID NO:22.  
     
     
         37 . The method of  claim 35  wherein said isolote is PS69D1 and said δ-endotoxin comprises SEQ ID NO:21.

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