Starch R1 phosphorylation protein homologs

Abstract

This invention relates to an isolated nucleic acid fragment encoding a starch R1 phosphorylation protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch R1 phosphorylation protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch R1 phosphorylation protein in a transformed host cell.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a polypeptide having starch R1 phosphorylation activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 18 have at least 85% sequence identity based on the Clustal alignment method, or    (b) a full-length complement of the nucleotide sequence of (a).    
     
     
         2 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 18 have at least 90% sequence identity based on the Clustal alignment method.  
     
     
         3 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 18 have at least 95% sequence identity based on the Clustal alignment method.  
     
     
         4 . The polynucleotide of  claim 1  wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 18.  
     
     
         5 . The polynucleotide of  claim 1 , wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO: 17.  
     
     
         6 . A vector comprising the polynucleotide of  claim 1 .  
     
     
         7 . A recombinant DNA construct comprising the polynucleotide of  claim 1  operably linked to at least one regulatory sequence.  
     
     
         8 . A method for transforming a cell, comprising transforming a cell with the polynucleotide of  claim 1 .  
     
     
         9 . A cell comprising the recombinant DNA construct of  claim 7 .  
     
     
         10 . A method for producing a plant comprising transforming a plant cell with the polynucleotide of  claim 1  and regenerating a plant from the transformed plant cell.  
     
     
         11 . A plant comprising the recombinant DNA construct of  claim 7 .  
     
     
         12 . A seed comprising the recombinant DNA construct of  claim 7 .  
     
     
         13 . An isolated polypeptide having starch R1 phosphorylation activity, wherein the polypeptide has an amino acid sequence of at least 85% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 18.  
     
     
         14 . The polypeptide of  claim 13 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 18.  
     
     
         15 . The polypeptide of  claim 13 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 18.  
     
     
         16 . The polypeptide of  claim 13 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO: 18.  
     
     
         17 . A method for isolating a polypeptide having starch R1 phosphorylation activity, wherein the method comprises: 
 transforming a cell with the recombinant DNA construct of  claim 7;     growing the cell in culture medium; and    isolating the polypeptide from the cell or culture medium.    
     
     
         18 . A method of altering the level of expression of a polypeptide having starch RI phosphorylation activity in a host cell comprising: (a) transforming a host cell with the recombinant DNA construct of  claim 7;  and (b) growing the transformed host cell under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of altered levels of the polypeptide in the transformed host cell.  
     
     
         19 . An isolated polynucleotide comprising: 
 (a) a first nucleotide sequence encoding a first polypeptide comprising at least 600 amino acids, wherein the amino acid sequence of the first polypeptide and the amino acid sequence of SEQ ID NO:20 have at least 80% identity based on the Clustal alignment method,    (b) a second nucleotide sequence encoding a second polypeptide comprising at least 1337 amino acids, wherein the amino acid sequence of the second polypeptide and the amino acid sequence of SEQ ID NO: 16 or SEQ ID NO: 18 have at least 80% identity based on the Clustal alignment method, or    (c) the complement of the first or second nucleotide sequence, wherein the complement and the first or second nucleotide sequence contain the same number of nucleotides and are 100% complementary.