US2004002109A9PendingUtilityA9
Process for identifying modulators of G-protein-coupled receptors
Priority: Jul 8, 2000Filed: Jul 6, 2001Published: Jan 1, 2004
Est. expiryJul 8, 2020(expired)· nominal 20-yr term from priority
Inventors:Evi Kostenis
C07K 14/705C12Q 1/68
50
PatentIndex Score
0
Cited by
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0
Claims
Abstract
The invention relates to a widely applicable process for identifying chemical compounds, which modulate G-protein-coupled receptors, by means of novel hybrid G-proteins with broad receptor specificity and very high expression and also to chemical compounds which can be identified by such a process.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism, wherein said process comprises the following steps:
a) providing at least one cell which contains at least one GPCR-dependent signal transduction pathway and which produces one or more than one G-protein; b) providing at least one chemical compound to be studied; c) contacting the cell of a) with one or more of the chemical compounds of b); d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the cell of a) by means of a signal transduction pathway-dependent measurable signal.
2 . The process as claimed in claim 1 , wherein the cell provided according to a) produces at least two G-proteins.
3 . The process as claimed in claim 1 , wherein the cell provided according to a) produces at least two G-proteins selected from -6qi4myr, -6qs5myr, -6qi4, -6qs5, and Gα16.
4 . The process as claimed in claim 2 , wherein the cell provided according to a) produces at least two G-proteins selected from -6qi4myr, -6qs5myr, -6qi4, -6qs5, and Gα16.
5 . The process as claimed in claim 1 , wherein the cell provided according to a) produces at least one G-protein selected from -6qi4myr, -6qs5myr, -6qi4, and -6qs5.
6 . The process as claimed in claim 2 , wherein the cell provided according to a) produces at least one G-protein selected from -6qi4myr, -6qs5myr, -6qi4, and -6qs5.
7 . The process as claimed in claim 1 , wherein the cell provided according to a) produces at least one protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
8 . The process as claimed in claim 2 , wherein the cell provided according to a) produces at least one protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
9 . The process as claimed in claim 3 , wherein the cell provided according to a) produces at least one protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
10 . The process as claimed in claim 4 , wherein the cell provided according to a) produces at least one protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
11 . The process as claimed in claim 5 , wherein the cell provided according to a) produces at least one protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
12 . The process as claimed in claim 6 , wherein the cell provided according to a) produces at least one protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
13 . The process as claimed in claim 1 , wherein the cell provided according to a) is the cell of a vertebrate species, an insect species, a yeast species, or a C. elegans.
14 . The process as claimed in claim 13 , wherein the cell provided is a HeLa, 293, COS or CHO cell, or a cell of Saccharomyces cerevisiae.
15 . The process as claimed in claim 1 , wherein the intracellular Ca2+ concentration is the signal transduction pathway-dependent measurable signal.
16 . The process as claimed in claim 2 , wherein the intracellular Ca2+ concentration is the signal transduction pathway-dependent measurable signal.
17 . The process as claimed in claim 3 , wherein the intracellular Ca2+ concentration is the signal transduction pathway-dependent measurable signal.
18 . The process as claimed in claim 4 , wherein the intracellular Ca2+ concentration is the signal transduction pathway-dependent measurable signal.
19 . The process as claimed in claim 5 , wherein the intracellular Ca2+ concentration is the signal transduction pathway-dependent measurable signal.
20 . The process as claimed in claim 6 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
21 . The process as claimed in claim 7 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
22 . The process as claimed in claim 8 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
23 . The process as claimed in claim 9 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
24 . The process as claimed in claim 10 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
25 . The process as claimed in claim 11 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
26 . The process as claimed in claim 12 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
27 . The process as claimed in claim 13 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
28 . The process as claimed in claim 14 , wherein the intracellular Ca 2+ concentration is the signal transduction pathway-dependent measurable signal.
29 . The process as claimed in claim 1 , wherein the process is used for identifying a pharmaceutical.
30 . The process as claimed in claim 2 , wherein the process is used for identifying a pharmaceutical.
31 . The process as claimed in claim 3 , wherein the process is used for identifying a pharmaceutical.
32 . The process as claimed in claim 4 , wherein the process is used for identifying a pharmaceutical.
33 . The process as claimed in claim 5 , wherein the process is used for identifying a pharmaceutical.
34 . The process as claimed in claim 6 , wherein the process is used for identifying a pharmaceutical.
35 . The process as claimed in claim 7 , wherein the process is used for identifying a pharmaceutical.
36 . The process as claimed in claim 8 , wherein the process is used for identifying a pharmaceutical.
37 . The process as claimed in claim 9 , wherein the process is used for identifying a pharmaceutical.
38 . The process as claimed in claim 10 , wherein the process is used for identifying a pharmaceutical.
39 . The process as claimed in claim 11 , wherein the process is used for identifying a pharmaceutical.
40 . The process as claimed in claim 12 , wherein the process is used for identifying a pharmaceutical.
41 . The process as claimed in claim 13 , wherein the process is used for identifying a pharmaceutical.
42 . The process as claimed in claim 14 , wherein the process is used for identifying a pharmaceutical.
43 . The process as claimed in claim 15 , wherein the process is used for identifying a pharmaceutical.
44 . The process as claimed in claim 16 , wherein the process is used for identifying a pharmaceutical.
45 . The process as claimed in claim 17 , wherein the process is used for identifying a pharmaceutical.
46 . The process as claimed in claim 18 , wherein the process is used for identifying a pharmaceutical.
47 . The process as claimed in claim 19 , wherein the process is used for identifying a pharmaceutical.
48 . The process as claimed in claim 20 , wherein the process is used for identifying a pharmaceutical.
49 . The process as claimed in claim 21 , wherein the process is used for identifying a pharmaceutical.
50 . The process as claimed in claim 22 , wherein the process is used for identifying a pharmaceutical.
51 . The process as claimed in claim 23 , wherein the process is used for identifying a pharmaceutical.
52 . The process as claimed in claim 24 , wherein the process is used for identifying a pharmaceutical.
53 . The process as claimed in claim 25 , wherein the process is used for identifying a pharmaceutical.
54 . The process as claimed in claim 26 , wherein the process is used for identifying a pharmaceutical.
55 . The process as claimed in claim 27 , wherein the process is used for identifying a pharmaceutical.
56 . The process as claimed in claim 28 , wherein the process is used for identifying a pharmaceutical.
57 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 1 .
58 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 2 .
59 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 3 .
60 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 4 .
61 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 5 .
62 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 6 .
63 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 7 .
64 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 8 .
65 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 9 .
66 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 10 .
67 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 11 .
68 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 12 .
69 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 13 .
70 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 14 .
71 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 15 .
72 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 16 .
73 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 17 .
74 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 18 .
75 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 19 .
76 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 20 .
77 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 21 .
78 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 22 .
79 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 23 .
80 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 24 .
81 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 25 .
82 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 26 .
83 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 27 .
84 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 28 .
85 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 29 .
86 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 30 .
87 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 31 .
88 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 32 .
89 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 33 .
90 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 34 .
91 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 35 .
92 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 36 .
93 . A compound which modifies the- action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 37 .
94 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 38 .
95 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 39 .
96 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 40 .
97 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 41 .
98 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 42 .
99 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 43 .
100 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 44 .
101 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 45 .
102 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 46 .
103 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 47 .
104 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 48 .
105 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 49 .
106 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 50 .
107 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 51 .
108 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 52 .
109 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 53 .
110 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 54 .
111 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 55 .
112 . A compound which modifies the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism and which is identified by the process as claimed in claim 56 .
113 . A polynucleotide sequence coding for a polypeptide having the property of a G-protein, wherein the polypeptide sequence is selected from:
a) SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8; b) a sequence according to a) lacking one or more amino acids; c) a sequence according to a) having an additional one or more amino acids; and d) an allelic variant of a sequence according to a).
114 . A polynucleotide comprising a polynucleotide sequence selected from:
a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, the corresponding sequence complementary thereto; and b) a polynucleotide sequence hybridizing with a polynucleotide sequence according to a) under stringent conditions.
115 . The polynucleotide as claimed in claim 113 , wherein the polynucleotide is part of a recombinant vector construct.
116 . The polynucleotide as claimed in claim 114 , wherein the polynucleotide is part of a recombinant vector construct.
117 . The polynucleotide as claimed in claim 115 , wherein the recombinant vector construct is an expression vector usable in eukaryotes and/or prokaryotes.
118 . The polynucleotide as claimed in claim 116 , wherein the recombinant vector construct is an expression vector usable in eukaryotes and/or prokaryotes.
119 . The polynucleotide as claimed in claim 117 , wherein the expression vector contains a constitutive and/or inducible promoter.
120 . The polynucleotide as claimed in claim 118 , wherein the expression vector contains a constitutive and/or inducible promoter.
121 . A host cell comprising a polynucleotide as claimed in claim 113 .
122 . A host cell comprising a polynucleotide as claimed in claim 114 .
123 . A host cell comprising a polynucleotide as claimed in claim 115 .
124 . A host cell comprising a polynucleotide as claimed in claim 116 .
125 . A host cell comprising a polynucleotide as claimed in claim 117 .
126 . A host cell comprising a polynucleotide as claimed in claim 118 .
127 . A host cell comprising a polynucleotide as claimed in claim 119 .
128 . A host cell comprising a polynucleotide as claimed in claim 120 .
129 . The host cell as claimed in claim 121 , wherein the host cell is a human cell.
130 . The host cell as claimed in claim 122 , wherein the host cell is a human cell.
131 . The host cell as claimed in claim 123 , wherein the host cell is a human cell.
132 . The host cell as claimed in claim 124 , wherein the host cell is a human cell.
133 . The host cell as claimed in claim 125 , wherein the host cell is a human cell.
134 . The host cell as claimed in claim 126 , wherein the host cell is a human cell.
135 . The host cell as claimed in claim 127 , wherein the host cell is a human cell.
136 . The host cell as claimed in claim 128 , wherein the host cell is a human cell.
137 . The host cell as claimed in claim 121 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
138 . The host cell as claimed in claim 122 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
139 . The host cell as claimed in claim 123 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
140 . The host cell as claimed in claim 124 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
141 . The host cell as claimed in claim 125 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
142 . The host cell as claimed in claim 126 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
143 . The host cell as claimed in claim 127 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
144 . The host cell as claimed in claim 128 , wherein the host cell is the cell of a vertebrate species, an insect species, a bacterial species, a yeast species, or C. elegans.
145 . The host cell as claimed in claim 137 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
146 . The host cell as claimed in claim 138 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
147 . The host cell as claimed in claim 139 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
148 . The host cell as claimed in claim 140 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
149 . The host cell as claimed in claim 141 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
150 . The host cell as claimed in claim 142 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
151 . The host cell as claimed in claim 143 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
153 . The host cell as claimed in claim 144 , wherein the cell is a HeLa, 293, COS or CHO cell, an Escherichia coli cell or Saccharomyces cerevisiae cell.
153 . A method of producing a host cell, wherein a polynucleotide as claimed in claim 115 is introduced into a eukaryotic or prokaryotic cell.
154 . A method of producing a host cell comprising a polynucleotide sequence selected from:
a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or the corresponding complementary sequence thereto; and b) a polynucleotide hybridizing with a polynucleotide sequence according to a) under stringent conditions, c) wherein a polynucleotide as claimed in claim 116 is introduced into a eukaryotic or prokaryotic cell.
155 . A method of producing a host cell comprising a polynucleotide sequence selected from:
a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or the corresponding complementary sequence thereto; and b) a polynucleotide hybridizing with a polynucleotide sequence according to a) under stringent conditions, c) wherein a polynucleotide as claimed in claim 117 is introduced into a eukaryotic or prokaryotic cell.
156 . A method of producing a host cell comprising a polynucleotide sequence selected from:
a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or the corresponding complementary sequence thereto; and b) a polynucleotide hybridizing with a polynucleotide sequence according to a) under stringent conditions, c) wherein a polynucleotide as claimed in claim 118 is introduced into a eukaryotic or prokaryotic cell.
157 . A method of producing a host cell, comprising a polynucleotide sequence selected from:
a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or the corresponding complementary sequence thereto; and b) a polynucleotide hybridizing with a polynucleotide sequence according to a) under stringent conditions, c) wherein a polynucleotide as claimed in claim 119 is introduced into a eukaryotic or prokaryotic cell.
158 . A method of producing a host cell, comprising a polynucleotide sequence selected from:
a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or the corresponding complementary sequence thereto; and b) a polynucleotide hybridizing with a polynucleotide sequence according to a) under stringent conditions, c) wherein a polynucleotide as claimed in claim 120 is introduced into a eukaryotic or prokaryotic cell.
159 . A method of using the host cell as claimed in claim 121 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
160 . A method of using the host cell as claimed in claim 122 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
161 . A method of using the host cell as claimed in claim 123 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
162 . A method of using the host cell as claimed in claim 124 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
163 . A method of using the host cell as claimed in claim 125 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
164 . A method of using the host cell as claimed in claim 126 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
165 . A method of using the host cell as claimed in claim 127 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
166 . A method of using the host cell as claimed in claim 128 in a process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism comprising:
a) providing said host cell;
b) providing at least one chemical compound to be studied;
c) contacting the host cell of a) with one or more of the chemical compounds of b);
d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the host cell of a) by means of a signal transduction pathway-dependent measurable signal.
167 . A protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:10.
168 . A process for preparing a protein as claimed in claim 167 comprising:
a) providing a host cell;
b) cultivating the host cell of a) in a growth medium suitable for the host cell and inducing expression of the protein;
c) disrupting the cells and obtaining the cell material;
d) removing the protein from other proteins of the disrupted cells of c).
169 . A method of using the protein as claimed in claim 167 for producing antibodies.
170 . A method of using the protein as claimed in claim 168 for producing antibodies.Join the waitlist — get patent alerts
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