Transcriptional regulator

Abstract

Genes each encoding a novel transcriptional regulator having a bromodomain have been successfully isolated from a human testis cDNA library using primers prepared based on an EST sequence found using the bromodomain sequence of the transcriptional regulator. These genes are structurally analogous to each other.

What is claimed is:  
     
         1 . A substantially pure polypeptide comprising an amino acid sequence at least 60% identical to any one of SEQ ID NOs: 1, 13, 21, 27, or 29, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.  
     
     
         2 . The polypeptide of  claim 1 , wherein the amino acid sequence is at least 70% identical to any one of SEQ ID NOs: 1, 13, 21, 27, or 29.  
     
     
         3 . The polypeptide of  claim 1 , wherein the amino acid sequence is at least 80% identical to any one of SEQ ID NOs: 1, 13, 21, 27, or 29.  
     
     
         4 . The polypeptide of  claim 1 , wherein the amino acid sequence is at least 90% identical to any one of SEQ ID NOs: 1, 13, 21, 27 or 29.  
     
     
         5 . A substantially pure polypeptide comprising any one of SEQ ID NOs: 1, 13, 21, 27, or 29.  
     
     
         6 . A substantially pure polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 1, 13, 21, 27, or 29, with up to 30 conservative amino acid substitutions, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.  
     
     
         7 . A substantially pure polypeptide encoded by a nucleic acid that hybridizes under high stringency conditions to a probe the sequence of which consists of any one of SEQ ID NOs: 2, 14, 22, 28, or 30, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.  
     
     
         8 . An isolated nucleic acid encoding the polypeptide of  claim 1 .  
     
     
         9 . An isolated nucleic acid encoding the polypeptide of  claim 5 .  
     
     
         10 . An isolated nucleic acid encoding the polypeptide of  claim 6 .  
     
     
         11 . An isolated nucleic acid comprising a strand that hybridizes under high stringency conditions to a single stranded probe consisting of any one of SEQ ID NOs: 2, 14, 22, 28, or 30.  
     
     
         12 . The isolated nucleic acid of  claim 11 , wherein the nucleic acid encodes a polypeptide that regulates transcription of a gene and comprises a bromodomain.  
     
     
         13 . The nucleic acid of  claim 12 , wherein the polypeptide comprises any one of SEQ ID NOs: 1, 13, 21, 27, or 29.  
     
     
         14 . The nucleic acid of  claim 11 , wherein the strand is at least 15 nucleotides in length.  
     
     
         15 . A vector comprising the nucleic acid of  claim 8 .  
     
     
         16 . A vector comprising the nucleic acid of  claim 9 .  
     
     
         17 . A vector comprising the nucleic acid of  claim 10 .  
     
     
         18 . A vector comprising the nucleic acid of  claim 11 .  
     
     
         19 . A vector comprising the nucleic acid of  claim 12 .  
     
     
         20 . A cultured host cell comprising the nucleic acid of  claim 8 .  
     
     
         21 . A cultured host cell comprising the nucleic acid of  claim 9 .  
     
     
         22 . A cultured host cell comprising the nucleic acid of  claim 10 .  
     
     
         23 . A cultured host cell comprising the nucleic acid of  claim 11 .  
     
     
         24 . A cultured host cell comprising the nucleic acid of  claim 12 .  
     
     
         25 . An antibody that specifically binds to the polypeptide of  claim 1 .  
     
     
         26 . A method of preparing a polypeptide, the method comprising culturing the host cell of  claim 20 , wherein the host cell expresses the polypeptide, and isolating the polypeptide from the host cell.  
     
     
         27 . A method of screening for a compound that binds to a polypeptide, the method comprising 
 providing a polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO: 1;    contacting a test compound with the polypeptide; and    determining whether the test compound has bound to the polypeptide.    
     
     
         28 . A compound which specifically binds to the polypeptide of  claim 1.