US2003221218A1PendingUtilityA1

Bioengineering cotton fiber properties

47
Assignee: UNIV CALIFORNIAPriority: May 17, 2002Filed: May 17, 2002Published: Nov 27, 2003
Est. expiryMay 17, 2022(expired)· nominal 20-yr term from priority
Inventors:Thea Wilkins
C07K 14/415C12N 9/88C12N 15/8243C12N 9/2437C12N 9/1007C12N 9/1051C12Y 302/01004Y02A40/146
47
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Claims

Abstract

The present invention provides plant fiber expansion (FE) genes that encode FE polypeptides, such as phosphoenol pyruvate carboxylase (PEPcase), expansin, endoglucanase, xyloglucan endoglycosyltransferase (XET), and pectin methyl esterase (PME). The invention further provides fiber-specific promoters. Still further, the invention provides molecular strategies for modulating fiber quality in fiber producing plants by modulating expression of FE genes or mutant forms of FE genes.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . An isolated nucleic acid molecule comprising a FE polynucleotide sequence, which is at least about 60% identical to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO: 5, SEQ ID NO: 7, or SEQ ID NO: 9.  
     
     
         2 . The isolated nucleic acid molecule of  claim 1 , wherein the isolated nucleic acid molecule comprises at least about 50 nucleotides in length.  
     
     
         3 . The isolated nucleic acid molecule of  claim 1 , wherein the isolated nucleic acid molecule comprises at least about 100 nucleotides.  
     
     
         4 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid encodes a PEPcase polypeptide having a sequence of SEQ ID NO:2.  
     
     
         5 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid encodes an expansin polypeptide having a sequence of SEQ ID NO:4.  
     
     
         6 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid encodes an endoglucanase polypeptide having a sequence of SEQ ID NO:6.  
     
     
         7 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid encodes an XET polypeptide having a sequence of SEQ ID NO:8.  
     
     
         8 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid encodes a PME polypeptide having a sequence of SEQ ID NO:10.  
     
     
         9 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid is a PEPcase polynucleotide as shown in SEQ ID NO:1.  
     
     
         10 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid is an expansin polynucleotide as shown in SEQ ID NO:3.  
     
     
         11 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid is an endoglucanase polynucleotide as shown in SEQ ID NO:5.  
     
     
         12 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid is an XET polynucleotide as shown in SEQ ID NO:7.  
     
     
         13 . The isolated nucleic acid molecule of  claim 1 , wherein the nucleic acid is a PME polynucleotide as shown in SEQ ID NO:9.  
     
     
         14 . The isolated nucleic acid molecule of  claim 1 , further comprising a plant promoter operably linked to the FE polynucleotide.  
     
     
         15 . The isolated nucleic acid molecule of  claim 14 , wherein the plant promoter is a tissue-specific promoter.  
     
     
         16 . The isolated nucleic acid molecule of  claim 15 , wherein the promoter is a fiber-specific promoter.  
     
     
         17 . The isolated nucleic acid molecule of  claim 14 , wherein the promoter is an inducible promoter.  
     
     
         18 . The isolated nucleic acid molecule of  claim 14 , wherein the FE polynucleotide is linked to the promoter in an antisense orientation.  
     
     
         19 . A transgenic plant comprising an expression cassette containing a plant promoter operably linked to a heterologous FE polynucleotide sequence, which is at least about 60% identical to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, and SEQ ID NO:9.  
     
     
         20 . The transgenic plant of  claim 19 , wherein the FE polynucleotide encodes an FE polypeptide.  
     
     
         21 . The transgenic plant of  claim 20 , wherein the FE polypeptide has an amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:10.  
     
     
         22 . The transgenic plant of  claim 19 , wherein the heterologous FE polynucleotide is linked to the promoter in an antisense orientation.  
     
     
         23 . The transgenic plant of  claim 19 , wherein the plant promoter is a tissue specific promoter.  
     
     
         24 . The transgenic plant of  claim 23 , wherein the promoter is a fiber-specific promoter.  
     
     
         25 . The transgenic plant of  claim 24 , wherein the promoter is a cotton fiber specific promoter.  
     
     
         26 . The transgenic plant of  claim 19 , wherein the FE polynucleotide has a nucleic acid sequence of SEQ ID NO:1.  
     
     
         27 . The transgenic plant of  claim 19 , wherein the FE polynucleotide has a nucleic acid sequence of SEQ ID NO:3.  
     
     
         28 . The transgenic plant of  claim 19 , wherein the FE polynucleotide has a nucleic acid sequence of SEQ ID NO:5.  
     
     
         29 . The transgenic plant of  claim 19 , wherein the FE polynucleotide has a nucleic acid sequence of SEQ ID NO:7.  
     
     
         30 . The transgenic plant of  claim 19 , wherein the FE polynucleotide has a nucleic acid sequence of SEQ ID NO:9.  
     
     
         31 . The transgenic plant of  claim 19 , wherein the plant is a member of the genus Gossypium.  
     
     
         32 . A method of modulating fiber quality in a plant, the method comprising introducing into the plant an expression cassette containing a plant promoter operably linked to a heterologous FE polynucleotide sequence, which is at least about 60% identical to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, and SEQ ID NO:9.  
     
     
         33 . The method of  claim 32 , wherein the heterologous FE polynucleotide encodes a FE polypeptide.  
     
     
         34 . The method of  claim 33 , wherein the FE polypeptide has an amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:10.  
     
     
         35 . The method of  claim 32 , wherein the heterologous FE polynucleotide is linked to the promoter in an antisense orientation.  
     
     
         36 . The method of  claim 32 , wherein the heterologous FE polynucleotide has a nucleic acid sequence of SEQ ID NO:1.  
     
     
         37 . The method of  claim 32 , wherein the plant promoter is a tissue specific promoter.  
     
     
         38 . The method of  claim 37 , wherein the promoter is a fiber specific promoter.  
     
     
         39 . The method of  claim 38 , wherein the promoter is a cotton fiber specific promoter.  
     
     
         40 . The method of  claim 32 , wherein the plant is a member of the genus Gossypium.  
     
     
         41 . The method of  claim 32 , wherein the expression cassette is introduced into the plant through a sexual cross.

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