Methods for improving the recovery of troponin I and T in membranes, filters and vessels

Abstract

A method to facilitate recovery troponin I and/or troponin T from a sample comprising addition of troponin C to the sample or to a surface from which the troponin I and/or troponin T are recovered.

What is claimed is:  
     
         1 . A method for facilitating the recovery of troponin I and/or troponin T from a sample, said method comprising steps of: 
 contacting the sample with a surface, and    adding troponin C to the surface or to the sample.    
     
     
         2 . The method of  claim 1  wherein the sample comprises whole blood, plasma or serum.  
     
     
         3 . The method of  claim 1  wherein the step of adding troponin C comprises adding a solution that comprises troponin C.  
     
     
         4 . The method of  claim 3  wherein the solution comprises 1 ng/ml to 1 mg/ml of troponin C.  
     
     
         5 . The method of  claim 3  wherein the solution comprises 0-1000 mole equivalents of calcium or magnesium.  
     
     
         6 . The method of  claim 5  wherein the calcium is provided as calcium chloride.  
     
     
         7 . The method of  claim 3  wherein the solution is added to the surface, and further comprising a step of drying the surface following addition of the solution.  
     
     
         8 . The method of  claim 1  wherein the surface comprises a membrane, a filter, a bibulous material, glass, plastic, a polymeric composition, a latex particle, a liposome, or a blood component.  
     
     
         9 . The method of  claim 8  wherein the blood component comprises a: very low density lipoprotein, low density lipoprotein, high density lipoprotein, or a protein of the coagulation cascade.  
     
     
         10 . The method of  claim 1  wherein the troponin C is of cardiac muscle or skeletal muscle origin.