US2003211543A1PendingUtilityA1

Enzyme kits and libraries

62
Priority: Jul 18, 1995Filed: May 19, 2003Published: Nov 13, 2003
Est. expiryJul 18, 2015(expired)· nominal 20-yr term from priority
Inventors:Jay M. Short
C12N 15/52C12Q 1/00C12N 15/1093C12N 15/1086
62
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

Recombinant enzyme libraries and kits where a plurality of enzymes are each characterized by different physical and/or chemical characteristics and classified by common characteristics. The characteristics are determined by screening of recombinant enzymes expressed by a DNA library produced from various microorganisms.

Claims

exact text as granted — not AI-modified
What is claimed:  
     
         1 . A method of screening clones having DNA recovered from a plurality of species of organisms for a specified enzyme activity, which method comprises: 
 screening for a specified enzyme activity in a library of clones prepared by 
 (i) recovering DNA from a DNA population derived from a plurality of species of organisms; and  
 (ii) transforming a host cell with the DNA of (i) to produce a library of clones which is screened for the specified enzyme activity.  
   
     
     
         2 . The method of  claim 1 , wherein the DNA is amplified prior to transforming the host cell.  
     
     
         3 . The method of  claim 1 , wherein the DNA is ligated into a vector prior to transforming the host cell.  
     
     
         4 . The method of  claim 3 , wherein the vector comprises at least one DNA sequence capable of regulating production of a detectable enzyme activity from said DNA.  
     
     
         5 . The method of  claim 3 , wherein the vector into which the DNA has been ligated is used to transform a host cell.

Join the waitlist — get patent alerts

Track US2003211543A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.