US2003211117A1PendingUtilityA1
Synthetic antigen for the detection of aids-related disease
Priority: Apr 29, 1985Filed: Nov 26, 2002Published: Nov 13, 2003
Est. expiryApr 29, 2005(expired)· nominal 20-yr term from priority
Inventors:Wesley L. Cosand
A61K 39/00C12N 2740/16222Y10S930/221Y10S435/961C07K 2319/00C07K 14/005Y10S435/974C12N 2740/16122C12N 15/66C07K 2319/02A61K 38/00
55
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Claims
Abstract
Novel peptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . In a method for detecting the presence of LAV/HTLV-III virus or antibody to LAV/HTLV-III virus where a sample is combined with a composition having epitopic sites immunologically competitive with LAV/HTLV-III epitopic sites, whereby antibodies bind to such protein composition to form a specific binding pair complex and the amount of complex formation is determined, the improvement which comprises:
employing in the assay medium as a reagent a compostion, containing at least one peptide which has at least five amino acids and fewer than 50 amino acids and is encoded for by the coding region of LAV/HTLV-III from bp 900 to bp 1421 or bp 7210 to bp 7815.
2 . A method according to claim 1 , wherein said coding region is from bp 1320 to bp 1388 or bp 7231 to bp 7794.
3 . A method according to claim 2 , wherein said composition includes at least two peptides, one encoded in the region bp 1320 to bp 1385 and the other encoded in the region bp 7231 to bp 7794.
4 . A method according to claim 3 , where each determination is made individually for the binding to each of the peptides.
5 . In a method for detecting the presence of LAV/HTLV-III virus or antibody to LAV/HTLV-III virus where a sample is combined with a composition having epitopic sites immunologically competitive with LAV/HTLV-III epitopic sites, whereby antibodies bind to each such protein composition to form at least one specific binding pair complex and the amount of complex formation is determined, the improvement which comprises:
employing in the assay medium as a reagent a composition, containing at least one peptide which has at least five amino acids in a sequence which comes within the sequence of at least one of the following peptide sequences: (I) (15) Y-Asp-Cys-Lys-Thr-Ile-Leu-Lys-Ala-Leu- Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Met-Met- Thr-Ala-Cys-X (II) (17) Y-Leu-Lys-Glu-Thr-Ile-Asn-Glu-Glu-Ala- Ala-Glu-Trp-Asp-Arg-Val-His-Pro-Val-His- Ala-Z-X (III) (92) Y-Asp-Arg-Val-His-Pro-Val-His-Ala-Gly-Pro-Ile-Ala-Pro- Gly-Gln-X (IV) (90) Y-Tyr-Ser-Pro-Thr-Ser-Ile-Leu-Asp-Ile-Arg- Gln-Gly-Pro-Lys-Glu-Pro-Phe-Arg-Asp-Tyr-Val- Asp-Arg-Phe-Tyr-Lys-Thr-Leu-Arg-Z-X (V) (88) Y-Asn-Trp-Nor-Thr-Glu-Thr-Leu-Leu-Val-Gln- Asn-Ala-Asn-Pro-Asp-Cys-Lys-Thr-Ile-Leu-Lys- Ala-Leu-Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Nor- Nor-Thr-Ala-Cys-X (VI) (97) Y-Arg-Glu-Leu-Glu-Arg-Phe-Ala-Val-Asn-Pro-Gly- Leu-Leu-Glu-Thr-Ser-Glu-Gly-Cys-Arg-Gln-Ile- Leu-Gly-Gln-Leu-Gln-Pro-Ser-Leu-Gln-Thr-X (VII) (71) Y-Asp-Thr-Gly-His-Ser-Ser-Gln-Val-Ser-Gln- Asn-Tyr (VIII) (36) Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro- Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg- Glu-Lys-Arg-Ala-Z-X (IX) (56) Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu- Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Z-X (X) (39) Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys- Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys- Ser-Gly-Lys-Leu-Ile-Cys-X (XI) (40) Y-Lys-Ser-Leu-Glu-Gln-Ile-Trp-Asn-Asn- Met-Thr-Trp-Met-Glu-Trp-Asp-Arg-Glu- Ile-Asn-Z-X (XII) (23) Y-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn- Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-Leu-Leu- Glu-Leu-Asp-Lys-Trp-Z-X (XIII) (79) Y-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly- Cys-Ser-Gly-Lys-Leu-Ile-Cys-X, where X is OH or NH 2 , and Y and Z, when present, are amino acids added to facilitate coupling, and where said peptide is free of other peptides or conjugated to a macromolecule for which antibodies in human sera are substantially absent.
6 . A method according to claim 5 , wherein said composition includes at least two of said peptides, at least one of said peptides having the amino acid sequence according to peptide sequences I to VII and at least one of said peptides having an amino acid sequence according to peptides VIII to XIII.
7 . A method according to claim 6 , wherein one of the peptides has one of the following sequences:
(VIIIa) (49)
Y-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-X
(VIIIb) (50)
Y-Pro-Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-
Gln-Arg-Glu-Lys-Arg-X
(IXa) (56/39)
Y-Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-
Lys-Asp-Gln-Gln-Z-X
(IXb) (39/56)
Y-Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu-Z-X
(XIIIa) (78)
Gly-Ile-Trp-Gly-Cys-Ser-Gly-Lys-Leu-Ile-Cys,
where X is OH or NH 2 , and Y, when present, is an amino acid added to facilitate coupling.
8 . A method according to claim 1 , wherein said composition includes at least one peptide selected from p25 and one from p18.
9 . A method according to claim 5 , wherein said composition is bound to a solid surface.
10 . A method for determining the presence of antibodies to LAV/HTLV-III in a physiological fluid, said method comprising:
introducing a human serum, plasma or blood sample into a sample container coated at least in part with at least one peptide having at least five amino acids which comes within the sequence of at least one of the following peptide sequences: (I) (15) Y-Asp-Cys-Lys-Thr-Ile-Leu-Lys-Ala-Leu- Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Met-Met- Thr-Ala-Cys-X (II) (17) Y-Leu-Lys-Glu-Thr-Ile-Asn-Glu-Glu-Ala- Ala-Glu-Trp-Asp-Arg-Val-His-Pro-Val-His- Ala-X (III) (92) Y-Asp-Arg-Val-His-Pro-Val-His-Ala-Gly-Pro Ile-Ala-Pro-Gly-Gln-X (IV) (90) Y-Tyr-Ser-Pro-Thr-Ser-Ile-Leu-Asp-Ile-Arg Gln-Gly-Pro-Lys-Glu-Pro-Phe-Arg-Asp-Tyr-Val- Asp-Arg-Phe-Tyr-Lys-Thr-Leu-Arg-Z-X (V) (88) y-Asn-Trp-Nor-Thr-Glu-Thr-Leu-Leu-Val-Gln- Asn-Ala-Asn-Pro-Asp-Cys-Lys-Thr-Ile-Leu-Lys- Ala-Leu-Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Nor- Nor-Thr-Ala-Cys-X (VI) (97) Y-Arg-Glu-Leu-Glu-Arg-Phe-Ala-Val-Asn-Pro-Gly- Leu-Leu-Glu-Thr-Ser-Glu-Gly-Cys-Arg-Gln-Ile- Leu-Gly-Gln-Leu-Gln-Pro-Ser-Leu-Gln-Thr-X (VII) (71) Y-Asp-Thr-Gly-His-Ser-Ser-Gln-Val-Ser-Gln- Asn-Tyr (VIII) (36) Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg Glu-Lys-Arg-Ala-Z-X (IX) (56) Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu- Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Z-X (X) (39) Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys- Ser-Gly-Lys-Leu-Ile-Cys-X (XI) (40) Y-Lys-Ser-Leu-Glu-Gln-Ile-Trp-Asn-Asn- Met-Thr-Trp-Met-Glu-Trp-Asp-Arg-Glu- Ile-Asn-Z-X (XII) (23) Y-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn- Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-Leu-Leu- Glu-Leu-Asp-Lys-Trp-Z-X (XIII) (79) Y-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly- Cys-Ser-Gly-Lys-Leu-Ile-Cys-X, where X is OH or NH 2 , and Y and Z, if present, are amino acids added to facilitate coupling, wherein said peptides are free of other peptides or conjugated to a macromolecule for which antibodies in human sera are substantially absent; incubating for a sufficient time for complex formation to occur; and determining the formation of complex by employing a labeled specific binding protein which binds to said complex and provides a detectable signal.
11 . A method according to claim 10 , wherein said peptide is conjugated to a water soluble protein of at least 5 kDal as said macromolecule.
12 . A method according to claim 10 , wherein two of said peptides are covalently linked together through a bond or chain.
13 . A method according to claim 10 , wherein said specific binding protein is antibody to human immunoglobulin.
14 . A method according to claim 10 , wherein said composition includes at least two of said peptides, at least one peptide coming from peptides I to VII and at least one peptide coming from peptides VIII to XIII.
15 . A method for determining the presence of antibodies to LAV/HTLV-III in a physiological fluid, said method comprising:
combining a human serum, plasma or blood sample with at least one labeled peptide having at least five amino acids in a sequence which comes within the sequence of at least one of the following peptide sequences: (I) (15) Y-Asp-Cys-Lys-Thr-Ile-Leu-Lys-Ala-Leu- Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Met-Met Thr-Ala-Cys-X (II) (17) Y-Leu-Lys-Glu-Thr-Ile-Asn-Glu-Glu-Ala- Ala-Glu-Trp-Asp-Arg-Val-His-Pro-Val-His- Ala-X (III) (92) Y-Asp-Arg-Val-His-Pro-Val-His-Ala-Gly-Pro- Ile-Ala-Pro-Gly-Gln-X (IV) (90) Y-Tyr-Ser-Pro-Thr-Ser-Ile-Leu-Asp-Ile-Arg- Gln-Gly-Pro-Lys-Glu-Pro-Phe-Arg-Asp-Tyr-Val- Asp-Arg-Phe-Tyr-Lys-Thr-Leu-Arg-z-x (V) (88) Y-Asn-Trp-Nor-Thr-Glu-Thr-Leu-Leu-Val-Gln- Asn-Ala-Asn-Pro-Asp-Cys-Lys-Thr-Ile-Leu-Lys- Ala-Leu-Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Nor- Nor-Thr-Ala-Cys-X (VI) (97) Y-Arg-Glu-Leu-Glu-Arg-Phe-Ala-Val-Asn-Pro-Gly- Leu-Leu-Glu-Thr-Ser-Glu-Gly-Cys-Arg-Gln-Ile- Leu-Gly-Gln-Leu-Gln-Pro-Ser-Leu-Gln-Thr-X (VII) (71) Y-Asp-Thr-Gly-His-Ser-Ser-Gln-Val-Ser-Gln- Asn-Tyr (VIII) (36) Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro- Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg Gln-Lys-Arg-Ala-Z-X (IX) (56) Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu- Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Z-X (X) (39) Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys- Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys- Ser-Gly-Lys-Leu-Ile-Cys-X (XI) (40) Y-Lys-Ser-Leu-Glu-Gln-Ile-Trp-Asn-Asn- Met-Thr-Trp-Met-Glu-Trp-Asp-Arg-Glu- Ile-Asn-Z-X (XII) (23) Y-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn- Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-Leu-Leu- Glu-Leu-Asp-Lys-Trp-Z-x (XIII) (79) Y-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly- Cys-Ser-Gly-Lys-Leu-Ile-Cys-X, where X is OH or NH 2 , and Y and Z, if present, are amino acids added to facilitate coupling, and said peptides are conjugated to a protein for which antibodies in human sera are substantially absent or unconjugated to a protein; incubating for a sufficient time for complex formation to occur; and determining the formation of complex as a result of change in a detectable signal resulting from complex formation.
16 . A method according to claim 15 , wherein said label is a fluorescer.
17 . A method according to claim 16 , wherein complex formation is determined by fluorescence polarization.
18 . A method according to claim 15 , wherein said label is an enzyme.
19 . A method according to claim 18 , wherein complex formation is determined as a result of enzyme activity modulation.
20 . A peptide of the formula:
(I) (15)
Y-Asp-Cys-Lys-Thr-Ile-Leu-Lys-Ala-Leu-
Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Met-Met-
Thr-Ala-Cys-X,
where X is OH or NH 2 , and Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated I, and I linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
21 . A peptide of the formula:
(II) (17)
Y-Leu-Lys-Glu-Thr-Ile-Asn-Glu-Glu-Ala
Ala-Glu-Trp-Asp-Arg-Val-His-Pro-Val-His
Ala-X,
where X is OH or NH 2 , and Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated II, and II linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
22 . A peptide of the formula:
(III) (92)
Y-Asp-Arg-Val-His-Pro-Val-His-Ala-Gly-Pro-
Ile-Ala-Pro-Gly-Gln-X,
where X is OH or NH 2 , and Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated III, and III linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
23 . A peptide of the formula:
(IV) (90)
Y-Tyr-Ser-Pro-Thr-Ser-Ile-Leu-Asp-Ile-Arg
Gln-Gly-Pro-Lys-Glu-Pro-Phe-Arg-Asp-Tyr-Val-
Asp-Arg-Phe-Tyr-Lys-Thr-Leu-Arg-Z-X,
where X is OH or NH 2 , and Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated IV, and IV linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
24 . A peptide of the formula:
(V) (88)
Y-Asn-Trp-Nor-Thr-Glu-Thr-Leu-Leu-Val-Gln-
Asn-Ala-Asn-Pro-Asp-Cys-Lys-Thr-Ile-Leu-Lys-
Ala-Leu-Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Nor-
Nor-Thr-Ala-CyS-X,
where X is OH or NH 2 , and Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated V, and V linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
25 . A peptide of the formula:
(VI) (97)
Y-Arq-Glu-Leu-Glu-Arg-Phe-Ala-Val-Asn-Pro-Gly-
Leu-Leu-Glu-Thr-Ser-Glu-Gly-Cys-Arg-Gln-Ile-
Leu-Gly-Gln-Leu-Gln-Pro-Ser-Leu-Gln-Thr-X,
where X is OH or NH 2 , and Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated VI, and VI linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
26 . A peptide of the formula:
(VII) (71)
Y-Asp-Thr-Gly-His-Ser-Ser-Gln-Val-Ser-Gln-
Asn-Tyr
where Y, if present, is an amino acid added to facilitate coupling, N-terminal acetylated VII, and VII linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
27 . A peptide of the formula:
(VIII) (36)
Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-
Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-
Glu-Lys-Arg-Ala-Z-X,
where X is OH or Nil 2 , and Z, if present, is an amino acid added to facilitate coupling, N-terminal acetylated VIII, and VIII linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
28 . A peptide of the formula:
(IX) (56)
Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu-
Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Z-X,
where X is OH or NH 2 , and Z, if present, is an amino acid added to facilitate coupling, N-terminal acetylated IX, and IX linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
29 . A peptide of the formula:
(X) (39)
Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys-
Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys-
Ser-Gly-Lys-Leu-Ile-Cys-X,
where X is OH or NH 2 , N-terminal acetylated X, and X linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
30 . A peptide of the formula:
(XI) (40)
Y-Lys-Ser-Leu-Glu-Gln-Ile-Trp-Asn-Asn-
Met-Thr-Trp-Met-Glu-Trp-Asp-Arg-Glu-
Ile-Asn-Z-X,
where X is OH or NH 2 , and each of Y and Z, if present, is an amino acid added to facilitate coupling, N-terminal acetylated XI, and XI linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
31 . A peptide of the formula:
(XII) (23)
Y-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn-
Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-Leu-Leu-
Glu-Leu-Asp-Lys-Trp-Z-X,
where X is OH or NH 2 , and each of Y and Z, if present, is an amino acid added to facilitate coupling, N-terminal acetylated XII, and XII linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
32 . A peptide of the formula:
(XIII) (79)
Y-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-
Cys-Ser-Gly-Lys-Leu-Ile-Cys-X,
where X is OH or NH 2 , and each of Y and Z. If present, is an amino acid added to facilitate coupling, N-terminal acetylated XIII, and XIII linked to a peptide or protein of at least 5,000 molecular weight, which peptide or protein does not normally bind to antibodies present in a human host.
33 . A vaccine composition comprising at least one peptide of the peptides:
(I) (15)
Y-Asp-Cys-Lys-Thr-Ile-Leu-Lys-Ala-Leu-
Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Met-Met
Thr-Ala-Cys-X
(II) (17)
Y-Leu-Lys-Glu-Thr-Ile-Asn-Glu-Glu-Ala-
Ala-Glu-Trp-Asp-Arg-Val-His-Pro-Val-His-
Ala-Z-X
(III) (92)
Y-Asp-Arg-Val-His-Pro-Val-His-Ala-Gly-Pro-
Ile-Ala-Pro-Gly-Gln-X
(IV) (90)
Y-Tyr-Ser-Pro-Thr-Ser-Ile-Leu-Asp-Ile-Arg-
Gln-Gly-Pro-Lys-Glu-Pro-Phe-Arg-Asp-Tyr-Val
Asp-Arg-Phe-Tyr-Lys-Thr-Leu-Arg-Z-x
(V) (88)
Y-Asn-Trp-Nor-Thr-Glu-Thr-Leu-Leu-Val-Gln-
Asn-Ala-Asn-Pro-Asp-Cys-Lys-Thr-Ile-Leu-Lys-
Ala-Leu-Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Nor-
Nor-Thr-Ala-Cys-X
(VI) (97)
Arg-Glu-Leu-Glu-Arg-Phe-Ala-Val-Asn-Pro-Gly-
Leu-Leu-Glu-Thr-Ser-Glu-Gly-Cys-Arg-Gln-Ile-
Leu-Gly-Gln-Leu-Gln-Pro-Ser-Leu-Gln-Thr
(VII) (71)
Asp-Thr-Gly-His-Ser-Ser-Gln-Val-Ser-Gln-Asn-Tyr
(VIII) (36)
Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-
Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-
Glu-Lys-Arg-Ala-Z-X
(IX) (56)
Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu-
Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Z-X
(X) (39)
Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys-
Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys-
Ser-Gly-Lys-Leu-Ile-Cys-X
(XI) (40)
Y-Lys-Ser-Leu-Glu-Gln-Ile-Trp-Asn-Asn-
Met-Thr-Trp-Met-Glu-Trp-Asp-Arg-Glu-
Ile-Asn-Z-X
(XII) (23)
Y-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn-
Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-Leu-Leu-
Glu-Leu-Asp-Lys-Trp-Z-X
(XIII) (79)
Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-
Cys-Ser-Gly-Lys-Leu-Ile-Cys,
where X is OH or NH 2 , and each of Y and Z. when present, are amino acids added to facilitate coupling,
or said peptides conjugated to an immunogenic protein, said peptides or conjugates being present in an amount to provide an immunogenic response in a physiologically acceptable carrier.
34 . A vaccine according to claim 34 , wherein said peptides are coupled to immunogenic proteins.
35 . A vaccine according to claim 34 , wherein at least one peptide is I to VII and at least one peptide is VIII to XIII.
36 . A vaccine according to any of claims 33 , 34 or 35 , wherein said immunogenic protein is tetanus toxoid.Join the waitlist — get patent alerts
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