US2003207411A1PendingUtilityA1

DNA sequence encoding enzymes of clavulanic acid biosynthesis

50
Assignee: UNIV ALBERTAPriority: Oct 8, 1993Filed: Jun 11, 2003Published: Nov 6, 2003
Est. expiryOct 8, 2013(expired)· nominal 20-yr term from priority
Inventors:Susan JensenKwamena A. AidooAshish Paradkar
C12P 17/188C12N 15/76C12N 15/52C07K 14/36
50
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

DNA sequences are provided which encode the enzymes required for clavulanic acid synthesis. A process is provided for producing clavulanic acid in a transformant of a non-clavulanate-producing host.

Claims

exact text as granted — not AI-modified
We claim:  
     
         1 . An isolated DNA molecule consisting of the nucleotide sequence selected from the group consisting of SEQ ID NO:15, 16, 19, 20, 21, and 22.  
     
     
         2 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:15.  
     
     
         3 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:16.  
     
     
         4 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:19.  
     
     
         5 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:20.  
     
     
         6 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:21.  
     
     
         7 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:22.  
     
     
         8 . An isolated DNA molecule consisting of a nucleotide sequence encoding the amino acid sequence selected from the group consisting of SEQ ID NO:4, 5, 8, 9, 10 and 11.  
     
     
         9 . The isolated DNA molecule of  claim 8  consisting of the nucleotide sequence of SEQ ID NO:4.  
     
     
         10 . The isolated DNA molecule of  claim 8  consisting of the nucleotide sequence of SEQ ID NO:5.  
     
     
         11 . The isolated DNA molecule of  claim 8  consisting of the nucleotide sequence of SEQ ID NO:8.  
     
     
         12 . The isolated DNA molecule of  claim 8  consisting of the nucleotide sequence of SEQ ID NO:9.  
     
     
         13 . The isolated DNA molecule of  claim 8  consisting of the nucleotide sequence of SEQ ID NO:10.  
     
     
         14 . The isolated DNA molecule of  claim 8  consisting of the nucleotide sequence of SEQ ID NO:11.  
     
     
         15 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:15 and 16, and excluding other reading frames.  
     
     
         16 . The isolated DNA molecule of  claim 1  consisting of the nucleotide sequence of SEQ ID NO:19, 20, 21 and 22 and excluding other reading frames.  
     
     
         17 . A recombinant vector comprising at least one of the isolated DNA molecules of  claim 1 .  
     
     
         18 . A recombinant vector comprising at least one of the isolated DNA molecules of  claim 8 .  
     
     
         19 . A host transformed with a recombinant vector in accordance with  claim 17 .  
     
     
         20 . A host in accordance with  claim 19  which is a Streptomycete.  
     
     
         21 . A host in accordance with  claim 20  which is  S. lividans.    
     
     
         22 . A host transformed with a recombinant vector in accordance with  claim 18 .  
     
     
         23 . A host in accordance with  claim 22  which is a Streptomycete.  
     
     
         24 . A host in accordance with  claim 23  which is  S. lividans.    
     
     
         25 . A process for producing clavulanic acid in a non-clavulanate-producing host comprising transforming the host with at least one of the DNA molecules of  claim 1 , and culturing the host under suitable conditions to produce clavulanic acid.  
     
     
         26 . A process for producing clavulanic acid in accordance with  claim 25  wherein the host is  S. lividans.    
     
     
         27 . A process for producing clavulanic acid in a non-clavulanate-producing host comprising transforming the host with at least one of the DNA molecules of  claim 8 , and culturing the host under suitable conditions to produce clavulanic acid.  
     
     
         28 . A process for producing clavulanic acid in accordance with  claim 27  wherein the host is  S. lividans.    
     
     
         29 . A process for enhancing clavulanic acid production in a clavulanate-producing host comprising transforming the host with a DNA molecule comprising a nucleotide sequence in accordance with  claim 1 .  
     
     
         30 . A process for enhancing clavulanic acid production in a clavulanate-producing host comprising transforming the host with a DNA molecule comprising a nucleotide sequence in accordance with  claim 8 .  
     
     
         31 . An isolated DNA molecule consisting of the nucleotide sequence of nucleotides 2257-5522 of SEQ ID NO:1.  
     
     
         32 . The isolated DNA molecule of  claim 31  consisting of the nucleotide sequence of nucleotides1765-6594 of SEQ ID NO:1.  
     
     
         33 . The isolated DNA molecule of  claim 34  consisting of the nucleotide sequence of nucleotides 7630-13809 of SEQ ID NO:1.  
     
     
         34 . An isolated DNA molecule consisting of the nucleotide sequence of nucleotides 7936-13,406 of SEQ ID NO:1.

Join the waitlist — get patent alerts

Track US2003207411A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.